Toehold-mediated strand displacement coupled with single nanoparticle dark-field microscopy imaging for ultrasensitive biosensing

Guo, Longhua; Xu, Shaohua; Wang, Yueliang; Yao, Yuanyuan; Chen, Lifen; Xu, Jiahui; Qiu, Bin

doi:10.1039/d1nr08030j

Cited by 6 publications

(1 citation statement)

References 32 publications

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“…This shows that DNA has a great potential to deal with difficult mathematical problems ( Chen et al, 2023 ). DNA strand displacement (DSD) is an efficient technology for DNA computing that provides rich intelligent toolboxes and has been widely used to solve many computational problems, such as NP problem ( Lipton, 1995 ), information encryption ( Sun et al, 2023 ; Wang et al, 2022a ; Zou et al, 2022 ; Sun et al, 2023 ), nanomachines ( Junke et al, 2020 ; Zhang et al, 2023b ), intelligent drug delivery ( Wang et al, 2022b ), clinical diagnosis ( Fan et al, 2022 ; Boskovic et al, 2023 ; Márquez-Costa et al, 2023 ), and biosensors ( Dong et al, 2022 ; Xu et al, 2022 ; Sun et al, 2023 ). With the rapid development of DNA strand displacement technology, several molecular logic circuits have been designed ( Wang et al, 2020 ; Zhu et al, 2020 ; Litovco et al, 2021 ; Chen et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular circuit for exponentiation based on the domain coding strategy

Huang,

Duan,

Guo

et al. 2024

Front. Genet.

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DNA strand displacement (DSD) is an efficient technology for constructing molecular circuits. However, system computing speed and the scale of logical gate circuits remain a huge challenge. In this paper, a new method of coding DNA domains is proposed to carry out logic computation. The structure of DNA strands is designed regularly, and the rules of domain coding are described. Based on this, multiple-input and one-output logic computing modules are built, which are the basic components forming digital circuits. If the module has n inputs, it can implement 2n logic functions, which reduces the difficulty of designing and simplifies the structure of molecular logic circuits. In order to verify the superiority of this method for developing large-scale complex circuits, the square root and exponentiation molecular circuits are built. Under the same experimental conditions, compared with the dual-track circuits, the simulation results show that the molecular circuits designed based on the domain coding strategy have faster response time, simpler circuit structure, and better parallelism and scalability. The method of forming digital circuits based on domain coding provides a more effective way to realize intricate molecular control systems and promotes the development of DNA computing.

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Section: Introductionmentioning

confidence: 99%

Molecular circuit for exponentiation based on the domain coding strategy

Huang,

Duan,

Guo

et al. 2024

Front. Genet.

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Innovative Progress of LSPR‐Based Dark‐Field Scattering Spectral Imaging in the Biomedical Assay at the Single‐Particle Level

Shi,

Wang,

et al. 2024

ChemistryOpen

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The growing demand for detection and sensing in the biomedical field is placing higher demands on technology. In clinical testing, it is expected to be able to realize both rapid large‐field imaging and analysis of single particles (or single molecules or single cells), and it is expected to be able to grasp both the unique individuality of single particles in time and space during the complex reaction process, as well as the regular correlation between single particles in the same population distribution. Supported and promoted by the theory of localized surface plasmon resonance (LSPR), dark‐field microscopy, as a single‐particle optical imaging technique with a very high signal‐to‐noise ratio, provides a powerful new means to address the above clinical detection needs. This review will focus on the innovative applications of dark‐field microscopy in biomedical‐related assays in the past five years, introducing the basic principles and listing the impressing works. We also summarize how dark‐field microscopy has been combined with other techniques, including surface‐enhanced Raman scattering, fluorescence, colorimetry, electrochemistry, etc., to witness the joint progress and promotion of detection methods in the future. It also provides an outlook on the current challenges and future trends in this field.

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An electrochemical determination strategy for miRNA based on bimetallic nanozyme and toehold-mediated DNA replacement procedure

Zhang

et al. 2023

Microchim Acta

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Toehold-mediated strand displacement coupled with single nanoparticle dark-field microscopy imaging for ultrasensitive biosensing

Abstract: The highly sensitive detection of biomarkers is essential for disease prevention and early diagnosis. Herein, a highly sensitive strategy was proposed for microRNA-21 (miRNA-21) detection by the incorporation of programmable...

Cited by 6 publications

References 32 publications

Molecular circuit for exponentiation based on the domain coding strategy

Molecular circuit for exponentiation based on the domain coding strategy

Innovative Progress of LSPR‐Based Dark‐Field Scattering Spectral Imaging in the Biomedical Assay at the Single‐Particle Level

An electrochemical determination strategy for miRNA based on bimetallic nanozyme and toehold-mediated DNA replacement procedure

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