Toll-like receptor 4/nuclear factor-kappa B pathway is involved in activating microphages by polysaccharides isolated from<i>Fagopyrum esculentum</i>

Chen, Zhe; Yu, Leilei; Cai, Xiaoniao; Ye, Fangpeng; Jin, Peisheng

doi:10.1080/21655979.2019.1682214

Cited by 3 publications

(2 citation statements)

References 32 publications

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“…Then, the conjugate solution was introduced and samples were incubated for 90 min, followed by introducing the TMB solution for 15 min. Finally, the reaction was terminated by adding the stop solution and the absorbance at 450 nm was measured by a microplate reader (BMG LABTECH, Offenburg, Germany) [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

Zafirlukast ameliorates Docetaxel-induced activation of NOD-like receptor protein 3 (NLRP3) inflammasome, mediated by sirtuin1 (SIRT1) in hepatocytes

2021

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Docetaxel-associated liver injury has become a serious public health problem, resulting in therapy discontinuation, liver failure, and death. Zafirlukast is a typical leukotriene receptor antagonist used for prophylaxis and chronic treatment of asthma. In this study, we investigate whether treatment with Zafirlukast could alleviate Docetaxel-induced cytotoxicity in hepatocytes. Our results indicate that Zafirlukast mitigated Docetaxel-induced toxicity in LO-2 hepatocytes. Firstly, Zafirlukast reduced the production of 8-hydroxy-2p-deoxyguanosine (8-OHdG) and increased the levels of reduced glutathione (GSH) against Docetaxel. Secondly, Zafirlukast elevated the levels of mitochondrial membrane potential (ΔΨm) and adenosine triphosphate (ATP). Thirdly, Zafirlukast prevented Docetaxel-induced release of lactate dehydrogenase (LDH) and increased cell viability of LO-2 hepatocytes against Docetaxel. We also found that Zafirlukast ameliorated Docetaxel-induced apoptosis by reducing Caspase-3 and Caspase-9 activity. Mechanistically, our results demonstrate that Zafirlukast inhibited the activation of NOD-like receptor protein 3 (NLRP3), mediated by SIRT1. Based on these findings, we conclude that the administration of Zafirlukast might have a protective effect against Docetaxel-induced cytotoxicity in hepatocytes.

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Section: Methodsmentioning

confidence: 99%

Zafirlukast ameliorates Docetaxel-induced activation of NOD-like receptor protein 3 (NLRP3) inflammasome, mediated by sirtuin1 (SIRT1) in hepatocytes

2021

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“…In recent years, plant polysaccharides have been extensively studied as immune stimulants in both cell and animal models to explore their immune regulatory activities [10,11]. Numerous studies found that the immunomodulatory mechanism of polysaccharides is mainly realized through interactions with the polysaccharide immune cell membrane receptors that trigger various molecular signaling cascades mediated by the cell signaling pathways that promote the secretion of immune factors [12,13]. Immune factors are essential for an animal's growth performance and health.…”

Section: Introductionmentioning

confidence: 99%

Dietary Artemisia Ordosica Polysaccharide Enhances Spleen and Intestinal Immune Response of Broiler Chickens

Du,

Xing,

et al. 2023

Biology

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The spleen and small intestines are the primary immune organs that provide important immunity against various diseases. Artemisia ordosica polysaccharide (AOP) could be used as an immunologic enhancer to boost immunity in response to infection. This study was performed to explore the effects of the dietary supplementation of AOP on the growth performance and spleen and small intestine immune function in broilers. A total of 288 AA broilers (1 day old) were randomly assigned into six dietary groups. Each group included six replicates of eight broilers per cage. The broilers were fed with a basal diet supplemented with 0 mg/kg (CON), 50 mg/kg chlortetracycline (CTC), 250, 500, 750, and 1000 mg/kg AOP for 42 d. The results showed that dietary AOP supplementation affected broiler growth performance, with 750 and 1000 mg/kg of AOP being able to significantly improve broiler BWG, and 750 mg/kg of AOP was able to significantly reduce the FCR. The dietary AOP supplementation increased the levels of IgA, IgG, IgM, IL-1β, IL-2, and IL-4 in the spleen and small intestine in a dose-dependent manner (p < 0.05). Meanwhile, we found that AOP can promote the mRNA expression of TLR4/MAPK/NF-κB signaling-pathway-related factors (TLR4, MyD88, P38 MAPK, JNK, NF-κB p50, and IL-1β). In addition, the dietary supplementation of 750 mg/kg AOP provides better immunity in the tissue than the CON group but showed no significant difference from the CTC group. Therefore, AOP has an immunoregulatory action and can modulate the immune function of broilers via the TLR4/ NF-ΚB/MAPK signal pathway. In conclusion, dietary supplementation with 750 mg/kg AOP may be alternatives to antibiotics for enhancing broilers’ health, immunity, and growth performance.

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Structural characteristics and immunomodulatory effects of polysaccharides extracted from plant seeds: A review

Dong,

Deng,

Wang

et al. 2024

Trends in Food Science & Technology

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Toll-like receptor 4/nuclear factor-kappa B pathway is involved in activating microphages by polysaccharides isolated fromFagopyrum esculentum

Cited by 3 publications

References 32 publications

Zafirlukast ameliorates Docetaxel-induced activation of NOD-like receptor protein 3 (NLRP3) inflammasome, mediated by sirtuin1 (SIRT1) in hepatocytes

Zafirlukast ameliorates Docetaxel-induced activation of NOD-like receptor protein 3 (NLRP3) inflammasome, mediated by sirtuin1 (SIRT1) in hepatocytes

Dietary Artemisia Ordosica Polysaccharide Enhances Spleen and Intestinal Immune Response of Broiler Chickens

Structural characteristics and immunomodulatory effects of polysaccharides extracted from plant seeds: A review

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