Toluene degradation via a unique metabolic route in indigenous bacterial species

Muccee, Fatima; Ejaz, Samina; Riaz, Naheed

doi:10.1007/s00203-019-01705-0

Cited by 29 publications

(15 citation statements)

References 64 publications

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“…For example, B. amyloliquefaciens is thought to promote plant growth through the biosynthesis of indole-3-acetic acid (IAA) molecules, enriching the available nitrogen, phosphate, and potassium in soil and improving soil health via a synergistic enhancement of several soil enzymes [30]. B. subtilis, on the other hand, is involved in the expression of specific genes and hormones, such as 1-aminocyclopropane-1-carboxylate deaminase (ACC) [31]. Of Brevibacillus spp., Br.…”

Section: Molecular Characterization Of the Isolatesmentioning

confidence: 99%

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Bacterial Biodiversity of Extra Virgin Olive Oils and Their Potential Biotechnological Exploitation

Fancello¹,

Multineddu²,

Santona³

et al. 2020

Microorganisms

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Bacterial diversity of 15 extra virgin olive oils, obtained from different Italian varieties, including Frantoio, Coratina, Bosana, and Semidana, was analyzed in this study. All bacterial isolates were genotyped using RAPD and REP-PCR method and grouped by means of cluster analyses. Sequencing of 16S rDNA of 51 isolates, representative of 36 clusters, led to the identification of Bacillus spp., Brevibacillus spp., Micrococcus spp., Staphylococcus spp., Pantoea spp., Kocuria spp., Lysinbacillus spp., and Lactobacillus spp., most of which reported for first time in olive oils. Phenotypic characterization of the 51 isolates, some of which ascribed to potentially probiotic species, indicate that two of them have beta-glucosidase activity while 37% present lipolytic activity. Preliminary evaluation of probiotic potential indicates that 31% of the isolates show biofilm formation ability, 29% acidic pH resistance, and 25% bile salt resistance. Finally, 29% of the isolates were sensitive to antibiotics while the remaining 71%, that include bacterial species well-recognized for their ability to disseminate resistance genes in the environment, showed a variable pattern of antibiotic resistance. The results obtained underline that microbial diversity of extra virgin olive oils represents an unexpected sink of microbial diversity and poses safety issues on the possible biotechnological exploitation of this microbial biodiversity.

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Section: Molecular Characterization Of the Isolatesmentioning

confidence: 99%

“…Of Brevibacillus spp., Br. agri strains have been found to tolerate and to be capable of degrading toluene [31], as well as able to reduce hexavalent chromium, preventing the generation of harmful byproducts [32]. A strain of Br.…”

Section: Molecular Characterization Of the Isolatesmentioning

confidence: 99%

Bacterial Biodiversity of Extra Virgin Olive Oils and Their Potential Biotechnological Exploitation

Fancello¹,

Multineddu²,

Santona³

et al. 2020

Microorganisms

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“…The lipolytic enzymes of B. subtilis and B. amyloliquefaciens are of significant biotechnological interest and, therefore, many of them have been identified, cloned, and characterized to date [34][35][36][37][38][39]. In addition, B. subtilis and B. amyloliquefaciens are known for their activity as plant growth promoting rhizobacteria (PGPR) and are widely used to stimulate growth and counteract numerous plant pathogens [40][41][42]. Another potential use of such bacteria is related to their probiotic characteristics [43].…”

Section: Phylogenetic Analysismentioning

confidence: 99%

Search for new cultured lipophilic bacteria in industrial fat-containing wastes

et al. 2020

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Fat-containing wastes that are generated as a result of industrial production of food products and are being accumulated in large quantities in wastewater and sewage treatment plants and present a serious environmental problem. Microorganisms that decompose various types of lipids may be potential candidates for creation of commercial bioformulations for fat destruction. The aim of the study was to obtain pure cultures of lipophilic bacteria from fat-containing wastes, to study their diversity and activity for the development of a biological product. As a result, 30 strains of different phylogenetic groups with lipolytic activity was obtained. The most isolated strains were represented by enterobacteria and pseudomonas members within the Gammaproteobacteria. Almost half of the isolated strains were closely related to conditionally pathogenic microorganisms such as Serratia, Klebsiella etc. Non-pathogenic strains and promising for biotechnology ones belonged to Pseudomonas citronellolis, P. nitroreducens, P. synxantha, P. extremaustralis, Bacillus subtilis, B. amyloliquefaciens, Brevibacillus brevis and Microvirgula sp.

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“…As a toxic volatile organic solvent, toluene is widely used in the production of daily life products, such as gasoline mixtures, benzene, cosmetics, ink, adhesives, paints, coatings, and glues [ 1 ]. It is difficult to degrade toluene by ultraviolet rays or atmospheric oxidation [ 2 ]. With the increase in population and development of cities, people stay indoors longer, and interior air quality has become more important.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Analysis for the Identification of Metabolic Pathway Genes Related to Toluene Response in Ardisia pusilla

Ahn

Shin

et al. 2021

Plants

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Toluene is an industrial raw material and solvent that can be found abundantly in our daily life products. The amount of toluene vapor is one of the most important measurements for evaluating air quality. The evaluation of toluene scavenging ability of different plants has been reported, but the mechanism of plant response to toluene is only partially understood. In this study, we performed RNA sequencing (RNA-seq) analysis to detect differential gene expression in toluene-treated and untreated leaves of Ardisiapusilla. A total of 88,444 unigenes were identified by RNA-seq analysis, of which 49,623 were successfully annotated and 4101 were differentially expressed. Gene ontology analysis revealed several subcategories of genes related to toluene response, including cell part, cellular process, organelle, and metabolic processes. We mapped the main metabolic pathways of genes related to toluene response and found that the differentially expressed genes were mainly involved in glycolysis/gluconeogenesis, starch and sucrose metabolism, glycerophospholipid metabolism, carotenoid biosynthesis, phenylpropanoid biosynthesis, and flavonoid biosynthesis. In addition, 53 transcription factors belonging to 13 transcription factor families were identified. We verified 10 differentially expressed genes related to metabolic pathways using quantitative real-time PCR and found that the results of RNA-seq were positively correlated with them, indicating that the transcriptome data were reliable. This study provides insights into the metabolic pathways involved in toluene response in plants.

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Toluene degradation via a unique metabolic route in indigenous bacterial species

Cited by 29 publications

References 64 publications

Bacterial Biodiversity of Extra Virgin Olive Oils and Their Potential Biotechnological Exploitation

Bacterial Biodiversity of Extra Virgin Olive Oils and Their Potential Biotechnological Exploitation

Search for new cultured lipophilic bacteria in industrial fat-containing wastes

Transcriptomic Analysis for the Identification of Metabolic Pathway Genes Related to Toluene Response in Ardisia pusilla

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