Toluidine Blue-Basic Fuchsin Stain for Glycolmethacrylate Embedded Tissue

Abreu, Mônica Azevedo de; Baroza, Lígia G.V.; Rossi, Marcos A.

doi:10.1179/his.1993.16.2.139

Cited by 22 publications

(11 citation statements)

References 3 publications

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“…Fragments of atria and ventricles from controls and infected animals (at day 12 of infection) were fixed in 4% paraformaldehyde in buffered phosphate, pH 7.3, 0.1 M, for 24 h, processed routinely and embedded in glycol methacrilate (Leica). Semi-serial 5-µm-thick sections stained by haematoxylin and eosin or toluidine bluebasic fuchsin (Abreu et al, 1993) were examined for qualitative evaluation of the inflammatory and degenerative processes and quantification of parasitism. The number of amastigote nests in cardiomyocytes was evaluated at 70 µm interval for avoiding parasite recounting (Hanson and Roberson, 1974).…”

Section: Light Microscopymentioning

confidence: 99%

Production of hydrogen peroxide by peripheral blood monocytes and specific macrophages during experimental infection with Trypanosoma cruzi in vivo

Melo

Fabrino

D’Avila

et al. 2003

Cell Biology International

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Acute Chagas' disease triggers potent inflammatory reaction characterized by great increase of peripheral blood monocyte (PBM) and macrophage numbers. We studied the respiratory burst responses of PBM and peritoneal and splenic macrophages to in vivo infection (rats). The ultrastructure of heart inflammatory macrophages was also investigated. The infection increased the hydrogen peroxide (H2O2) production by PBM and splenic macrophages but not by peritoneal macrophages. Accordingly, the PBM and spleen cell numbers increased but the total number of peritoneal cells was similar to controls. Heart macrophages of infected rats exhibited increase (number and size) and activated morphology in parallel to high cardiomyocyte parasitism. Our data highlight the importance of innate immunity and H2O2production to host resistance during acute phase of T. cruzi infection. A novel finding is that H2O2production seems related to specific types of monocytes/macrophages that are able to release this agent when in presence of high parasite load.

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Section: Light Microscopymentioning

confidence: 99%

Production of hydrogen peroxide by peripheral blood monocytes and specific macrophages during experimental infection with Trypanosoma cruzi in vivo

Melo

Fabrino

D’Avila

et al. 2003

Cell Biology International

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“…In the toluidine blue‐basic fuchsin staining method (Abreu et al, ; Gerrits and Smid, ), where tissues are stained after infiltration with glycomethacrylate (Technovit, 7200), similar staining pattern to that of H&E staining of decalcified was obtained and is, therefore, recommended for application in similar histopathological studies. Burr and Hooser () reported that toluidine blue‐basic fuchsin staining is prone to overstaining or understaining and thus requires tight control of staining times.…”

Section: Discussionmentioning

confidence: 99%

Comparative study of decalcification versus nondecalcification for histological evaluation of one‐wall periodontal intrabony defects in dogs

Park

Choi

Han³

et al. 2014

Microscopy Res & Technique

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Biological reactions between biomaterials and surrounding tissues, analyzed by histology, may be important predictors of clinical healing pattern and selection of slide preparation techniques requires a careful consideration regarding sample properties. In this study, we compared histology of bone specimens prepared with or without decalcification and performed histological and histomorphometrical assessments. For the histological evaluation, one-wall intrabony defects were created around the mandibular molars of six adult dogs, filled with biphasic calcium phosphate, synthetic bone graft material/recombinant human bone morphogenic protein-2, and healing pattern was histologically evaluated at 4 and 12 weeks. New bone formation in 5 × 4 × 4 mm defects and the length of new cementum, connective tissue attachments around the teeth and number of osteoclasts were measured by histomorphometric analysis. After decalcification, new cementum was easily observed and was significantly increased at week 4. In nondecalcified samples, significantly increased connective tissue attachments were seen at week 12. After 12 weeks, the number of countable multinucleated osteoclasts was significantly increased by 62% in nondecalcified versus calcified tissue sections (P = 0.030). Histomorphometric results may be significantly affected by histological preparation method and therefore, selecting the most appropriate histological preparation method is essential for reliable diagnosis and evaluation of bony samples in studies analyzing tissue regeneration.

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“…This point out that hemodilution with albumin 3% was a safe and more adequate procedure than the use of the schemes of administration of dextran-40 and enoxaparin used in this study to prevent thrombus formation at femoral vein microanastomosis in rats. Since hemodilution promotes reduction in blood viscosity and in erythrocyte and platelet aggregation as well as dilution of the coagulation factors themselves, 5,[13][14][15][16][17][18][19][20] it could provide better microcirculatory blood perfusion, decreasing the risk of thrombosis, and making possible safer microsurgical procedures. 21,22 …”

Section: Discussionmentioning

confidence: 99%

“…The sections were stretched on water, mounted on glass slides, and stained with a toluidine-blue-basic fuchsin stain. 13 Transverse sections of the anastomosis area were examined at the suture site for the absence or presence of thrombosis (thrombus adherent to the vein wall) (Fig. 2).…”

Section: Thrombosis Modelmentioning

confidence: 99%

Comparative study of isovolemic hemodilution with 3% albumin, dextran-40, and prophylactic enoxaparin (LMWH) on thrombus formation at venous microanastomosis in rats

et al. 2006

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The objective of the present investigation was to compare the effect of isovolemic hemodilution with 3% albumin, dextran-40, and enoxaparin on the prevention of thrombosis in femoral vein microanastomosis using an experimental model in rats. Forty male Wistar rats were allocated into four groups: group 1, control, thrombogenic model without previous treatment; group 2, hemodiluted, thrombogenic model with previous hemodilution; group 3, dextran-40, thrombogenic model with dextran infusion (10 ml/kg), and group 4, enoxaparin, thrombogenic model with administration of enoxaparin (0.5 mg/kg/day). Hemostatic parameters, hematologic examinations, patency of anastomosis, and histopathological examination were evaluated. The hemostatic parameters were similar in the four groups studied. Group hemodiluted, dextran-40, and enoxaparin showed significantly reduced number of red blood cells and platelets as compared with the control group. The hemodilution significantly increased the patency rates of the vein at 20 min and 48 h. Dextran-40 and enoxaparin improved the patency of the vein only at 20 min, but failed to show a significant increase in the final patency at 48 h. After 48 h, the rate of venous thrombosis, as evaluated microscopically, was significantly decreased in hemodiluted animals (1/8) as compared with controls (10/10); in rats treated with dextran-40 (7/10) and enoxaparin (5/10) the rate of venous thrombosis was significantly higher as compared with rats of the group hemodiluted. Based on these observations, it can be concluded that hemodilution with albumin 3% was a safe and more adequate procedure than the use of the schemes of administration of dextran-40 and enoxaparin used in this study to prevent thrombus formation at femoral vein microanastomosis in rats. Since hemodilution promotes reduction in blood viscosity and in erythrocyte and platelet aggregation as well as dilution of the coagulation factors themselves, its use could provide better microcirculatory blood perfusion, decreasing the risk of thrombosis, and making possible safer microsurgical procedures.

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Toluidine Blue-Basic Fuchsin Stain for Glycolmethacrylate Embedded Tissue

Cited by 22 publications

References 3 publications

Production of hydrogen peroxide by peripheral blood monocytes and specific macrophages during experimental infection with Trypanosoma cruzi in vivo

Production of hydrogen peroxide by peripheral blood monocytes and specific macrophages during experimental infection with Trypanosoma cruzi in vivo

Comparative study of decalcification versus nondecalcification for histological evaluation of one‐wall periodontal intrabony defects in dogs

Comparative study of isovolemic hemodilution with 3% albumin, dextran-40, and prophylactic enoxaparin (LMWH) on thrombus formation at venous microanastomosis in rats

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