Tomato: a crop species amenable to improvement by cellular and molecular methods

Hille, Jacques; Koornneef, M.; Ramanna, M.S.; Zabel, P.

doi:10.1007/bf00042609

Cited by 41 publications

(14 citation statements)

References 178 publications

(161 reference statements)

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“…Its moderately sized genome (950 Mb with hundreds of mapped traits and molecular markers), tolerance to inbreeding, amenability to genetic transformation, and diversity of secondary metabolism make tomato an excellent platform for genetic and molecular research (Tanksley, 1993). Compared with other commercial crop plants, a relatively large number of singlegene-determined traits have been described in tomato, with an estimate of 1200 available monogenic traits (Stevens and Rick, 1986;Hille et al, 1989). Map-based cloning recently resulted in the first cloned quantitative trait loci in tomato (Frary et al, 2000;Fridman et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Activation Tagging in Tomato Identifies a Transcriptional Regulator of Anthocyanin Biosynthesis, Modification, and Transport

et al. 2003

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We have developed a high-throughput T-DNA insertional mutagenesis program in tomato using activation tagging to identify genes that regulate metabolic pathways. One of the activation-tagged insertion lines ( ant1 ) showed intense purple pigmentation from the very early stage of shoot formation in culture, reflecting activation of the biosynthetic pathway leading to anthocyanin accumulation. The purple coloration resulted from the overexpression of a gene that encodes a MYB transcription factor. Vegetative tissues of ant1 plants displayed intense purple color, and the fruit showed purple spotting on the epidermis and pericarp. The gene-to-trait relationship of ant1 was confirmed by the overexpression of ANT1 in transgenic tomato and in tobacco under the control of a constitutive promoter. Suppression subtractive hybridization and RNA hybridization analysis of the purple tomato plants indicated that the overexpression of ANT1 caused the upregulation of genes that encode proteins in both the early and later steps of anthocyanidin biosynthesis as well as genes involved in the glycosylation and transport of anthocyanins into the vacuole.

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Section: Introductionmentioning

confidence: 99%

Activation Tagging in Tomato Identifies a Transcriptional Regulator of Anthocyanin Biosynthesis, Modification, and Transport

et al. 2003

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“…Currently, tomato is one of the main vegetable crops around the world [14]. It is grown both in tropical, subtropical and temperate areas [15]. Light is the main environmental factor affecting tomato growth and biomass production.…”

Section: Methodsmentioning

confidence: 99%

Manifestation of effect of fluctuating asymmetry of bilateral traits of tomato growing in industrial greenhouses

Ракутько

Alsiņa

Avotiņš

et al. 2018

Engineering for Rural Development

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Abstract. Sustainable plant development, i.e. maintenance of its phenotype in conditions of changing environmental parameters or genetic deviations, is realized by a set of mechanisms, generally characterized by developmental stability. The most striking manifestation of the stability of the development of the biological object at the macro level is the effect of fluctuating asymmetry (FA). The lighting conditions, along with other environmental factors, affect both the productivity of the plant and the stability of its development. The issues that remain scarcely studied are the influence of lighting conditions and plant variety on the FA level. One of the widely used and informative parameters is the chlorophyll content in plant leaves and their size. The aim of the study is to confirm the hypothesis of the effect of differences in the light quality and plant variety on the level of FA of the symmetrical plant structures. The object of the study is the leaves of tomato plants grown in an industrial greenhouse. The measurements were carried out in the greenhouse of Mezvidi, Latgalia, for three variants: tomato variety orange Bolzano F1 grown under high-pressure sodium lamps (HPS) and LED sources, and the red Encore F1 grown under HPS. As a result of the experiments, the presence of a significant asymmetry of the bilateral structures of tomato leaves was detected by their size and chlorophyll content. The fluctuating nature of asymmetry is proved. It was revealed that the influence of the type of the light source and tomato variety on FA on the basis of certain traits is multidirectional. For the tomato variety Bolzano, the level of FA on the basis of the leaflet size is 66 % higher under HPS compared to the LED, and on the basis of the chlorophyll content does not depend on the type of the light source. For HPS the level of FA on the basis of the leaflet size is 24 % higher at Bolzano compared to Encore, and on the basis of the chlorophyll content, it is 28 % lower.Keywords: developmental, stability, plant, lighting, energoecology. IntroductionEffective cultivation of plants in artificial conditions is possible only using the indoor plant lighting technology with supplemental light sources. Light is one of the factors that affect the concentration of phytochemical compounds [1]. Sustainable plant development, i.e. maintenance of its phenotype in conditions of changing environmental parameters or genetic deviations is realized by a set of mechanisms, generally characterized by stability of development. The most striking manifestation of the stability of the development of the biological object at the macrolevel is the fluctuating asymmetry (FA), which consists in minor and random deviations of the parameters of bilateral (mirror) traits. It was revealed that the relationship between stress factors and the stability of development is not obvious. A number of researchers note an increase of the FA level under stress, while others do not confirm a statistically significant relationship [2]. Under favourable environ...

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“…además, se han utilizado rFLPs para el monitoreo de clones en el banco de germoplasma in vitro del utilizado rFLPs para el monitoreo de clones en el banco de germoplasma in vitro de Biodiversity international (antes centro internacional para recursos Vegetales genéticos, iPgri por sus siglas en inglés) con el fin de asegurarse que se están conservando plantas que mantienen íntegras sus características fenotí-picas (Withers et al 1990). También se ha empleado esta técnica para estudios de la variación somaclonal in vitro, clonación de genes y la detección de transposones en tomate (Solanum lycopersicum) (Hille et al 1989). otros ejemplos relacionados con el empleo de esta técnica se presentan en el cuadro 1.…”

Section: Polimorfismos En La Longitud De Los Fragmentos De Restriccciunclassified

Técnicas moleculares para la detección de variantes somaclonales

Chiang

Jiménez

2008

Agron. Mesoam.

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resumenTécnicas moleculares para la detección de variantes somaclonales. La variación somaclonal es la variación genética o epigenética que se genera durante el cultivo in vitro de plantas (cultivos celulares de tejidos u órganos) que provenga de células somáticas. en programas de mejoramiento genético, la variación somaclonal puede constituirse en un recurso importante que genera variabilidad. sin embargo, durante la micropropagación y en bancos de germoplasma in vitro, este tipo de variación es indeseable. en vista de lo anterior, se han utilizado una serie de técnicas moleculares para su detección. En esta revisión bibliográfica se presenta una descripción de las técnicas moleculares útiles en la detección de variación somaclonal in vitro y, además, una recopilación de trabajos publicados sobre la detección de variación somaclonal por medio de técnicas moleculares. palabras clave: cambios epigenéticos, cultivo de tejidos, marcadores moleculares, mutaciones, variabilidad. aBsTracT molecular techniques for the detection of somaclonal variation. soma-clonal variation comprises genetic or epigenetic changes that arise during the in vitro culture phase of somatic cells (cell, tissue, organ cultures, etc.). in plant breeding programs, soma-clonal variation might become an important source of variability. However, this kind of variation is undesirable for micro-propagation purposes and for germplasm conservation in vitro, where genetic fidelity is desired. Therefore, a series of molecular techniques has been used for detection of soma-clonal variation. This review presents a description of the main molecular techniques used to detect in vitro soma-clonal variation. moreover, a compilation of scientific papers related to the detection of soma-clonal variation by molecular techniques is presented.Key words: epigenetic variation, tissue culture, molecular markers, mutations, variability. inTroducciónLa variación somaclonal se refiere a la variación que ocurre in vitro en cultivos celulares, de tejidos y órganos, así como en plantas regeneradas de los mismos. La variación somaclonal generalmente es espontánea y los cambios pueden ser heredables o

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Tomato: a crop species amenable to improvement by cellular and molecular methods

Cited by 41 publications

References 178 publications

Activation Tagging in Tomato Identifies a Transcriptional Regulator of Anthocyanin Biosynthesis, Modification, and Transport

Activation Tagging in Tomato Identifies a Transcriptional Regulator of Anthocyanin Biosynthesis, Modification, and Transport

Manifestation of effect of fluctuating asymmetry of bilateral traits of tomato growing in industrial greenhouses

Técnicas moleculares para la detección de variantes somaclonales

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