Tomato cv. ‘Micro-Tom’ as a model system to study postharvest chilling tolerance

González, Carla; Ré, Martín D.; Sossi, María L.; Valle, Estela M.; Boggio, Silvana B.

doi:10.1016/j.scienta.2014.12.020

Cited by 21 publications

(29 citation statements)

References 28 publications

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“…Tomato fruits, cvs. Micro‐Tom and Minitomato, differing in tolerance to postharvest chilling (Gonzalez et al ), were selected for analysis of the sHsps expression during fruit ripening. The transcript level of the cytoplasmic sHsps, sHsp 17.4‐CII, sHsp 17.6‐CI, sHsp 17.6‐CII , and sHsp 17.7‐CI and organellar sHsps, sHsp 21.5‐ER localized in the endoplasmic reticulum, sHsp 21‐P in the chloroplast, and sHsp 23.8‐M in the mitochondria were analyzed by qRT‐PCR in the pericarp of mature tomato fruit.…”

Section: Resultsmentioning

confidence: 99%

“…Micro‐Tom and Minitomato fruits show different characteristic after postharvest chilling. While Micro‐Tom fruit is chilling‐tolerant, Minitomato fruit develops chilling injury symptoms after chilling storage (Gonzalez et al ). The fact that chilling injury symptoms become evident after the product is returned to ambient temperature (Sharom et al ) suggests that the physiological events that occur the first days after chilling are determinant for the subsequent fruit behavior.…”

Section: Resultsmentioning

confidence: 99%

“…Fruit ripening occurred in three different conditions: on the vine (fruits were allowed to ripen naturally on the plant), off the vine (fruits were picked at the mature green stage and directly placed on a shelf in the growing cabinet), and prechilled (fruits were harvested at the mature green stage, stored for 4 weeks at 4°C, and then transferred back to the growing cabinet). Fruits were collected at the mature green (G), yellow (Y), orange (O), and red (R) stages (Gonzalez et al ). G ch corresponds to green fruit conserved for 4 weeks at 4°C.…”

Section: Methodsmentioning

confidence: 99%

“…Membrane fluidity and permeability at low temperature (Nishida and Murata ), alteration in the cell‐wall metabolism (Brummell et al ), and oxidative stress (Malacrida et al ) have been related to the chilling injury. Moreover, there is evidence of the existence of genetic variability in low‐temperature sensitivity within Solanum species such as potato (Palta et al ) and tomato (Gonzalez et al ). Several reports have suggested that various genes related to different stress responses, including small heat shock protein ( sHsp ) genes, may have a role in the acquirement of chilling tolerance.…”

Section: Introductionmentioning

confidence: 99%

“…To gain more insight into the molecular mechanisms involved in chilling tolerance, an investigation on the sHsps and Hsfs expression in tomato fruit while ripening on the vine, off the vine, and after cold storage was performed. A comparative approach was used employing two tomato varieties with contrasting postharvest chilling tolerance (Gonzalez et al ): cv. Micro‐Tom that is a model system to study postharvest chilling tolerance and cv.…”

Section: Introductionmentioning

confidence: 99%

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Small heat shock proteins and the postharvest chilling tolerance of tomato fruit

Ré

González

Escobar

et al. 2016

Physiologia Plantarum

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Plants have the largest number of small heat shock proteins (sHsps) (15-42 kDa) among eukaryotes, but little is known about their function in vivo. They accumulate in response to different stresses, and specific sHsps are also expressed during developmental processes such as seed development, germination, and ripening. The presence of organelle-specific sHsps appears to be unique to plants. The sHsps expression is regulated by heat stress transcription factors (Hsfs). In this work, it was explored the role of sHsps in the chilling injury of tomato fruit. The level of transcripts and proteins of cytoplasmic and organellar sHsps was monitored in fruit during ripening and after cold storage (4 weeks at 4°C). Expression of HsfA1, HsfA2, HsfA3, and HsfB1 was also examined. Two cultivars of tomato (Solanum lycopersicum) contrasting in chilling tolerance were assayed: Micro-Tom (chilling-tolerant) and Minitomato (chilling-sensitive). Results showed that sHsps were induced during ripening in fruit from both cultivars. However, sHsps were induced in Micro-Tom fruit but not in Minitomato fruit after storage at a low temperature. In particular, sHsp 17.4-CII and sHsp23.8-M transcripts strongly accumulated in Micro-Tom fruit and HsfA3 transcript diminished after cold storage. These data suggest that sHsps may be involved in the protection mechanisms against chilling stress and substantiate the hypothesis that sHsps may participate in the mechanism of tomato genotype chilling tolerance.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Small heat shock proteins and the postharvest chilling tolerance of tomato fruit

Ré

González

Escobar

et al. 2016

Physiologia Plantarum

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Reversible changes in galactolipid saturation level and head group composition are associated with tolerance to postharvest chilling in tomato fruit

2021

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BACKGROUND Chilling injury (CI) is a physiological disorder that results in a limitation for cold storage (CS) of many fruits and vegetables. The low temperature‐induced changes in the properties and composition of cell membranes are involved in the response to chilling temperature and in the mechanism of CI and tolerance. RESULTS We compared the changes in the lipid composition by gas chromatography‐mass spectrometry before, immediately after CS, as well as during a 3‐day subsequent period, of tomato fruits with different chilling‐sensitivity: Micro‐Tom (tolerant) and Minitomato (susceptible). The changes in linolenic acid content, double bond index and digalactosyldiacylglycerol/monogalactosyldiacylglycerol ratio (DGDG/MGDG) showed membrane fluidity adjustment, depending on the temperature. By a database search, we identified 18 membrane‐bound fatty acid desaturase (FAD) genes and five DGDG synthases (DGD) genes that phylogenetically clustered into four and two subfamilies, respectively. The FAD and DGD genes were differentially expressed in response to CS, as determined by quantitative reverse transcriptase‐polymerase chain reaction analysis. CONCLUSION The data strongly suggest that reversion of CS‐induced changes during the recovery period is important for the proper function of the membrane and tolerance to postharvest CI in tomato fruit. © 2021 Society of Chemical Industry.

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Ploidy Breeding in Ornamentals

Eeckhaut

Veken

Dhooghe

et al. 2018

Handbook of Plant Breeding

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Tomato cv. ‘Micro-Tom’ as a model system to study postharvest chilling tolerance

Cited by 21 publications

References 28 publications

Small heat shock proteins and the postharvest chilling tolerance of tomato fruit

Small heat shock proteins and the postharvest chilling tolerance of tomato fruit

Reversible changes in galactolipid saturation level and head group composition are associated with tolerance to postharvest chilling in tomato fruit

Ploidy Breeding in Ornamentals

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