Tomato mottle mosaic virus: Characterization, resistance gene effectiveness, and quintuplex RT-PCR detection system

Tettey, Carlos Kwesi; Yan, Zhi‐Yong; Ma, Hua-Yu; Zhao, Meisheng; Geng, Chao; Tian, Yanping; LI, Xiang-dong

doi:10.1016/j.jia.2022.07.020

Cited by 7 publications

(7 citation statements)

References 25 publications

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“…Previous studies have developed ToMMV detection methods based on RT-PCR [ 15 , 52 ] and real-time RT-PCR [ 42 ]. These methods involve sequence analysis of amplified products, which provide valuable information on the lineages, mutations, and other relevant genetic features of viruses.…”

Section: Discussionmentioning

confidence: 99%

A Reverse-Transcription Loop-Mediated Isothermal Amplification Technique to Detect Tomato Mottle Mosaic Virus, an Emerging Tobamovirus

Kimura

Miyazaki

Suzuki

et al. 2023

Viruses

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Tomato mottle mosaic virus (ToMMV) is an emerging seed-transmissible tobamovirus that infects tomato and pepper. Since the first report in 2013 in Mexico, ToMMV has spread worldwide, posing a serious threat to the production of both crops. To prevent the spread of this virus, early and accurate detection of infection is required. In this study, we developed a detection method for ToMMV based on reverse-transcription loop-mediated isothermal amplification (RT-LAMP). A LAMP primer set was designed to target the genomic region spanning the movement protein and coat protein genes, which is a highly conserved sequence unique to ToMMV. This RT-LAMP detection method achieved 10-fold higher sensitivity than conventional RT-polymerase chain reaction methods and obtained high specificity without false positives for closely related tobamoviruses or healthy tomato plants. This method can detect ToMMV within 30 min of direct sampling of an infected tomato leaf using a toothpick and therefore does not require RNA purification. Given its high sensitivity, specificity, simplicity, and rapidity, the RT-LAMP method developed in this study is expected to be valuable for point-of-care testing in field surveys and for large-scale testing.

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Section: Discussionmentioning

confidence: 99%

A Reverse-Transcription Loop-Mediated Isothermal Amplification Technique to Detect Tomato Mottle Mosaic Virus, an Emerging Tobamovirus

Kimura

Miyazaki

Suzuki

et al. 2023

Viruses

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“…Moreover, the effectors might evolve distinct specificities to sequence-unrelated effectors by adopting a similar fold. Indeed, gaining a structural understanding of how these NLRs bind to effectors could offer more insights into the molecular mechanisms of direct perception [ 117 , 118 ].…”

Section: Underlying Mechanisms To Counter Pathogen Attackmentioning

confidence: 99%

“…Numerous active loss-of-susceptibility mechanisms are employed to develop resistance against viruses in plants. For instance, the Tm-2 gene product of tomato provides resistance against the tomato mosaic virus and inhibits replication by binding to replication proteins [ 118 ]. Furthermore, the resistance genes Ty-1/Ty-3 in wild tomatoes encode γ-clade RNA-directed RNA polymerases.…”

Section: Underlying Mechanisms To Counter Pathogen Attackmentioning

confidence: 99%

Molecular Basis of Plant–Pathogen Interactions in the Agricultural Context

Ijaz,

Zhao,

Shabala

et al. 2024

Biology

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Biotic stressors pose significant threats to crop yield, jeopardizing food security and resulting in losses of over USD 220 billion per year by the agriculture industry. Plants activate innate defense mechanisms upon pathogen perception and invasion. The plant immune response comprises numerous concerted steps, including the recognition of invading pathogens, signal transduction, and activation of defensive pathways. However, pathogens have evolved various structures to evade plant immunity. Given these facts, genetic improvements to plants are required for sustainable disease management to ensure global food security. Advanced genetic technologies have offered new opportunities to revolutionize and boost plant disease resistance against devastating pathogens. Furthermore, targeting susceptibility (S) genes, such as OsERF922 and BnWRKY70, through CRISPR methodologies offers novel avenues for disrupting the molecular compatibility of pathogens and for introducing durable resistance against them in plants. Here, we provide a critical overview of advances in understanding disease resistance mechanisms. The review also critically examines management strategies under challenging environmental conditions and R-gene-based plant genome-engineering systems intending to enhance plant responses against emerging pathogens. This work underscores the transformative potential of modern genetic engineering practices in revolutionizing plant health and crop disease management while emphasizing the importance of responsible application to ensure sustainable and resilient agricultural systems.

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“…Although using natural virus-resistant germplasm resources for hybrid breeding is the preferred and most effective way for obtaining resistant commercial varieties, only a few virus-resistant tomato germplasm resources are available, and the resistance is limited to a few viruses [74]. For example, virus resistance hybrid breeding cannot be applied against emerging viruses, such as ToCV, ToBRFV, ToMMV, etc., due to the lack of their corresponding natural resistance genes [14][15][16].…”

Section: The Challenges In Breeding Tomato Hybrid Cultivars Against V...mentioning

confidence: 99%

“…However, most of the virus resistance genes currently found in tomatoes are single-gene-mediated with qualitative resistance, and thus, virus mutation and mixed infection can easily lead to resistance breakdown [11][12][13]. In addition, some emerging tomato viruses, such as tomato chlorosis virus (ToCV), tomato brown rugose fruit virus (ToBRFV), and tomato mottle mosaic virus (ToMMV), still lack relevant natural resistance genes, making conventional virus resistance breeding in tomatoes challenging [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Advances and Prospects of Virus-Resistant Breeding in Tomatoes

Shahriari,

Su,

Zheng

et al. 2023

IJMS

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Plant viruses are the main pathogens which cause significant quality and yield losses in tomato crops. The important viruses that infect tomatoes worldwide belong to five genera: Begomovirus, Orthotospovirus, Tobamovirus, Potyvirus, and Crinivirus. Tomato resistance genes against viruses, including Ty gene resistance against begomoviruses, Sw gene resistance against orthotospoviruses, Tm gene resistance against tobamoviruses, and Pot 1 gene resistance against potyviruses, have been identified from wild germplasm and introduced into cultivated cultivars via hybrid breeding. However, these resistance genes mainly exhibit qualitative resistance mediated by single genes, which cannot protect against virus mutations, recombination, mixed-infection, or emerging viruses, thus posing a great challenge to tomato antiviral breeding. Based on the epidemic characteristics of tomato viruses, we propose that future studies on tomato virus resistance breeding should focus on rapidly, safely, and efficiently creating broad-spectrum germplasm materials resistant to multiple viruses. Accordingly, we summarized and analyzed the advantages and characteristics of the three tomato antiviral breeding strategies, including marker-assisted selection (MAS)-based hybrid breeding, RNA interference (RNAi)-based transgenic breeding, and CRISPR/Cas-based gene editing. Finally, we highlighted the challenges and provided suggestions for improving tomato antiviral breeding in the future using the three breeding strategies.

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Tomato mottle mosaic virus: Characterization, resistance gene effectiveness, and quintuplex RT-PCR detection system

Cited by 7 publications

References 25 publications

A Reverse-Transcription Loop-Mediated Isothermal Amplification Technique to Detect Tomato Mottle Mosaic Virus, an Emerging Tobamovirus

A Reverse-Transcription Loop-Mediated Isothermal Amplification Technique to Detect Tomato Mottle Mosaic Virus, an Emerging Tobamovirus

Molecular Basis of Plant–Pathogen Interactions in the Agricultural Context

Advances and Prospects of Virus-Resistant Breeding in Tomatoes

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