Tomato yellow leaf curl virus: No evidence for replication in the insect vector Bemisia tabaci

Sánchez-Campos, Sonia; Rodríguez-Negrete, Edgar A.; Cruzado, L.; Grande-Pérez, Ana; Bejarano, Eduardo R.; Navas‐Castillo, Jesús; Moriones, Enrique

doi:10.1038/srep30942

Cited by 36 publications

(27 citation statements)

References 42 publications

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“…In the second experiment [ 62 ], to prevent the acquisition of viral ssDNA and the dsDNA replicative form from plants that may stick to the capsid, B. tabaci B and Q were membrane-fed with DNAase I-treated purified TYLCV and TYLCV-Mld virions. Despite these precautions, both ssDNA and dsDNA were found associated with the particles.…”

Section: Organs Tissues and Cells Visited By Begomoviruses Circulmentioning

confidence: 99%

The Incredible Journey of Begomoviruses in Their Whitefly Vector

Czosnek

Hariton-Shalev

Sobol

et al. 2017

Viruses

172

125

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Begomoviruses are vectored in a circulative persistent manner by the whitefly Bemisia tabaci. The insect ingests viral particles with its stylets. Virions pass along the food canal and reach the esophagus and the midgut. They cross the filter chamber and the midgut into the haemolymph, translocate into the primary salivary glands and are egested with the saliva into the plant phloem. Begomoviruses have to cross several barriers and checkpoints successfully, while interacting with would-be receptors and other whitefly proteins. The bulk of the virus remains associated with the midgut and the filter chamber. In these tissues, viral genomes, mainly from the tomato yellow leaf curl virus (TYLCV) family, may be transcribed and may replicate. However, at the same time, virus amounts peak, and the insect autophagic response is activated, which in turn inhibits replication and induces the destruction of the virus. Some begomoviruses invade tissues outside the circulative pathway, such as ovaries and fat cells. Autophagy limits the amounts of virus associated with these organs. In this review, we discuss the different sites begomoviruses need to cross to complete a successful circular infection, the role of the coat protein in this process and the sites that balance between virus accumulation and virus destruction.

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Section: Organs Tissues and Cells Visited By Begomoviruses Circulmentioning

confidence: 99%

The Incredible Journey of Begomoviruses in Their Whitefly Vector

Czosnek

Hariton-Shalev

Sobol

et al. 2017

Viruses

172

125

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“…Lifetime infectivity persistence, alterations to whitefly biology, and accumulation of viral DNA and transcripts in the vector have been proposed as evidence of replication of TYLCV in the whitefly vector ( 17 – 21 ). However, contrasting evidence has been reported by two studies, suggesting that TYLCV does not replicate within whiteflies ( 22 , 23 ). In these previous studies, time-course quantification of viral load using qPCR showed that TYLCV DNA loads within whole whiteflies remained stable or slightly decreased after viral acquisition had stopped.…”

Section: Discussionmentioning

confidence: 70%

“…A notable exception is tomato yellow leaf curl virus (TYLCV), one of the most devastating viral pathogens of tomato that has spread to more than 50 countries and regions ( 15 ). The possible replication of TYLCV in the whitefly vector has received considerable attention since 1994 ( 17 – 23 ). Whereas several studies provided evidence supporting replication of TYLCV in B. tabaci ( 17 – 21 ), other studies have reported that the virus does not replicate in the insect vector ( 22 , 23 ).…”

mentioning

confidence: 99%

A plant DNA virus replicates in the salivary glands of its insect vector via recruitment of host DNA synthesis machinery

Wang

Yin

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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Whereas most of the arthropod-borne animal viruses replicate in their vectors, this is less common for plant viruses. So far, only some plant RNA viruses have been demonstrated to replicate in insect vectors and plant hosts. How plant viruses evolved to replicate in the animal kingdom remains largely unknown. Geminiviruses comprise a large family of plant-infecting, single-stranded DNA viruses that cause serious crop losses worldwide. Here, we report evidence and insight into the replication of the geminivirus tomato yellow leaf curl virus (TYLCV) in the whitefly (Bemisia tabaci) vector and that replication is mainly in the salivary glands. We found that TYLCV induces DNA synthesis machinery, proliferating cell nuclear antigen (PCNA) and DNA polymerase δ (Polδ), to establish a replication-competent environment in whiteflies. TYLCV replication-associated protein (Rep) interacts with whitefly PCNA, which recruits DNA Polδ for virus replication. In contrast, another geminivirus, papaya leaf curl China virus (PaLCuCNV), does not replicate in the whitefly vector. PaLCuCNV does not induce DNA-synthesis machinery, and the Rep does not interact with whitefly PCNA. Our findings reveal important mechanisms by which a plant DNA virus replicates across the kingdom barrier in an insect and may help to explain the global spread of this devastating pathogen.

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“…tabaci in a persistent, circulative manner. Although kinetic quantification of the accumulation of both complementary and virion sense viral DNA molecules within B. tabaci using two step qPCR procedure showed no significant increase of viral DNA accumulation in the insect vector 30 , an increased amount of viral DNA corresponding to genes from both strands of TYLCV was detected by real-time RT-PCR in individuals of B. tabaci following 8 h of AAP on TYLCV-infected tomato plants or purified virions and then transferred to non TYLCV-host cotton plants 31 . Accumulation of viral DNA and viral transcripts was also observed using Fluorescence in situ hybridization analysis 31 .…”

Section: Discussionmentioning

confidence: 89%

Molecular variation of tomato yellow leaf curl virus in the insect vector Bemisia tabaci

Yang

Luan

et al. 2017

Sci Rep

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Insect vectors play significant roles in geminivirus spread and evolution in nature. To date little is known about the population dynamics of begomoviruses in their insect vector Bemisia tabaci. In this study we analyzed the genetic variation of tomato yellow leaf curl virus (TYLCV) in its host plant, Solanum lycopersicum, in its transmission vector B. tabaci raised on TYLCV-infected S. lycopersicum plants, and in B. tabaci after being transferred from S. lycopersicum to Gossypium hirsutum. We found that the levels of variability of TYLCV remained stable in S. lycopersicum plants, but increased significantly in both invasive and indigenous species of B. tabaci. We also presented evidence that the elevated mutation frequencies in TYLCV populations from vector whiteflies were caused mainly by mutations that occurred at several distinct sites within the TYLCV genome. Simultaneous introduction of mutations in the hot spots did not affect the ability of TYLCV to be transmitted by B. tabaci, but reduced its pathogenicity in both S. lycopersicum and Nicotiana benthamiana. Our findings provide new information on population variability of TYLCV in its insect vector, extending the knowledge of the influence of insect vector on plant virus population dynamics.

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Tomato yellow leaf curl virus: No evidence for replication in the insect vector Bemisia tabaci

Cited by 36 publications

References 42 publications

The Incredible Journey of Begomoviruses in Their Whitefly Vector

The Incredible Journey of Begomoviruses in Their Whitefly Vector

A plant DNA virus replicates in the salivary glands of its insect vector via recruitment of host DNA synthesis machinery

Molecular variation of tomato yellow leaf curl virus in the insect vector Bemisia tabaci

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