Tonsil-derived mesenchymal stromal cells: evaluation of biologic, immunologic and genetic factors for successful banking

Ryu, Kyung Ha; Cho, Kyung Ah; Park, Hae Sang; Kim, Ji Yon; Woo, So Youn; Jo, Inho; Choi, Yoon Hee; Park, Young Mi; Jung, Sung Chul; Chung, Sung Min; Choi, Byung Ok; Kim, Hansu

doi:10.3109/14653249.2012.706708

Cited by 127 publications

(200 citation statements)

References 25 publications

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“…In this study, we compared T-MSC properties before and after cryopreservation at P2 and P15 and found no significant differences in morphology, expression of cell surface markers, proliferation ability and differentiation potential into osteogenic, adipogenic and chondrogenic tissues. These results are consistent with those of Ryu et al [8]. These results support the feasibility of the future use of T-MSCs as allogenic and/or autologous sources of cell-based therapy following cryopreservation.…”

Section: Discussionsupporting

confidence: 92%

“…The human palatine tonsil and adenoid are readily accessible to otolaryngologists and are easily obtained by tonsillectomy or punch biopsy, particularly in young donors. Recent studies isolated tonsil-MSCs (T-MSCs) and adenoid-MSCs (A-MSCs) from their respective tissues and found that the morphology, cell-surface markers, proliferation potential and differentiation potential of T-MSCs and A-MSCs were similar to those of BM-MSCs [5,7,8]. Also, T-MSCs displayed the immunomodulatory effects that are associated with inhibiting T-cell proliferation, decreasing the production of soluble factors, and increasing HLA-G5 secretion in response to various stimuli [9].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Donor Age, Long-Term Passage Culture, and Cryopreservation on Tonsil-Derived Mesenchymal Stem Cells

Choi

Lee²,

Park³

et al. 2015

Cell Physiol Biochem

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Objectives: Human mesenchymal stem cells (MSCs) are efficacious in various cellular therapeutic applications and have been isolated from several tissues. Recent studies have reported that human tonsil tissue contains a new source of progenitor cells, potentially applicable for cell-based therapies. Information about the effects of donor age, long-term passage and cryopreservation are essential for clinical applications and cell-based therapies. Therefore, the authors investigated how the morphology, cell-surface markers, proliferation potential and differentiation capacity of tonsil-derived MSCs (T-MSCs) were affected by donor age, long-term passage, and cryopreservation. Materials and Methods: T-MSCs were isolated from tonsillar tissue of 20 patients undergoing tonsillectomy. Authors evaluated the effects of donor-age, long-term passage, and cryopreservation on the morphology, surface markers, proliferation potential and differentiation capacities of T-MSCs. Results: T-MSCs exhibited a fibroblast-like, spindle-shaped appearance. There were no significant morphological differences according to donor age, long-term passage or cryopreservation. T-MSCs isolated from donors of various ages were positive for markers CD90, CD44, and CD73, but negative for CD45, CD31, and HLA-DR. There were no significant differences in the expression of positive and negative surface markers as a function of donor age, long-term passage and cryopreservation. T-MSCs from different donor age groups showed similar proliferation potentials after passage 2. After long-term passage and cryopreservation, there were no significant morphological differences. Cryopreservation did not affect the proliferation potential of T-MSCs, but there was a significant decrease in the proliferation potential in long-term passage T-MSCs (passage 15). The effect of donor age, long-term passage and cryopreservation on the in vitro adipogenic, osteogenic, and chondrogenic differentiation potential of T-MSCs was not significant. Conclusion: The effect of donor age, long-term passage culture, and cryopreservation on T-MSC properties are negligible, except for the proliferation capacity of long-term cultured T-MSCs. Therefore, T-MSCs are considered to be promising MSCs that can be used as future alternative sources for autologous or allogenic MSCs.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Effects of Donor Age, Long-Term Passage Culture, and Cryopreservation on Tonsil-Derived Mesenchymal Stem Cells

Choi

Lee²,

Park³

et al. 2015

Cell Physiol Biochem

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“…TMSC isolation and culture were conducted as previously described [17]. Briefly, a total of five patients younger than 10 years undergoing tonsillectomy (3 boys and 2 girls, mean age 6.2 years) were recruited at random from consecutive patients presenting between April 2012 and December 2012 at the Department of OtorhinolaryngologyeHead and Neck Surgery, Ewha Womans University Medical Center (EWUMC, Seoul, Korea).…”

Section: Isolation and Culture Of Tmscmentioning

confidence: 99%

“…Specifically, there are ethical issues associated with the use of hESC, while isolation of thymic stromal cells is rather invasive, and it takes over 10 weeks for thymic stromal cells to differentiate and secrete PTH. We previously isolated new stem cells from palatine tonsillar tissues, tonsil-derived mesenchymal stem cells (TMSC), and reported that TMSC have the potential to differentiate into endoderm tissues expressing the PTH gene [17]. In this study, we further investigated whether TMSC can be used for the development of functional parathyroid cells in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

Differentiated tonsil-derived mesenchymal stem cells embedded in Matrigel restore parathyroid cell functions in rats with parathyroidectomy

et al. 2015

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“…Recent studies reported the isolation of tonsilMSCs (T-MSCs) from tonsil tissues and found that the morphology, cell-surface markers, proliferation potential, and differentiation potential of T-MSCs were similar to those of BMMSCs [11,15]. T-MSCs were considered to be promising MSCs that could be used as future alternative sources for autologous or allogenic MSCs, because the effects of donor age, longterm passage culture, and cryopreservation on T-MSC properties were negligible [11,15]. Despite the reports of T-MSCs as a new autologous or homologous source of MSCs, there have been no reports on specific markers of MSCs for purified T-MSC isolation.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Localization of Mesenchymal Stem Cells in Human Palatine Tonsil by W5C5 (SUSD2)

Lee

Kang²,

Park³

et al. 2016

Cell Physiol Biochem

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Background/Aims: Although tonsil-mesenchymal stem cells (T-MSCs) have been studied as a new autologous or homologous source of MSCs, research on specific markers of MSCs and localization for purified T-MSC isolation has not yet been reported. This study investigates the expression of W5C5 (SUSD2) in tonsil stromal cells and the colony-forming ability and differentiation potential of W5C5+ cells to determine the usefulness of W5C5+ MSCs as a marker that can be used for the purification of T-MSCs. In addition, the location of W5C5+ cells expressed in the tonsil tissues is examined. Methods: T-MSCs were isolated from the tonsillar tissues of 12 patients undergoing tonsillectomy. The colony-forming ability, surface markers, proliferation potential, and differentiation capacities of purified W5C5+ MSCs, W5C5- MSCs, and unselected T-MSCs were evaluated. The location of the W5C5+ cells in the tonsillar tissues was also investigated by immunohistochemistry. Results: W5C5 was expressed in 2.5±0.4% of fresh human tonsil stromal cells. W5C5+ cells formed many colonies, but W5C5- cells did not form any colonies. The colony-forming number of W5C5+ cells (74.4 ± 9.8) was significantly higher than that of unselected tonsil stromal cells (23.6 ± 3.7). However, the differences in proliferation potential, surface marker expression, and differentiation potential between W5C5+ T-MSCs and unselected T-MSCs were not significant. W5C5+ cells were identified in the perivascular area around the blood vessels. Conclusion: W5C5+ T-MSCs possessed typical MSC properties with high colony-forming efficiency, and niches of W5C5+ T-MSCs were located in the perivascular area of tonsil tissues. These findings suggest that W5C5 is a useful single marker for the isolation of purified T-MSCs.

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Tonsil-derived mesenchymal stromal cells: evaluation of biologic, immunologic and genetic factors for successful banking

Cited by 127 publications

References 25 publications

Effects of Donor Age, Long-Term Passage Culture, and Cryopreservation on Tonsil-Derived Mesenchymal Stem Cells

Effects of Donor Age, Long-Term Passage Culture, and Cryopreservation on Tonsil-Derived Mesenchymal Stem Cells

Differentiated tonsil-derived mesenchymal stem cells embedded in Matrigel restore parathyroid cell functions in rats with parathyroidectomy

Isolation and Localization of Mesenchymal Stem Cells in Human Palatine Tonsil by W5C5 (SUSD2)

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