Toolbox for Antibiotics Discovery from Microorganisms

Fisch, Katja M.; Schäberle, Till F.

doi:10.1002/ardp.201600064

Cited by 11 publications

(9 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In some cases, dietary cyanobacteria or other bacteria have been proven to be the source of compounds, but, in general, origin from symbionts remains difficult to prove [ 481 ]. In any case, if NPs can be traced to a microorganism, this may help to solve the supply problems for many of the bioactive NPs, either by culturing, by isolating the BGCs (biosynthetic gene clusters), or by using other metagenomic techniques [ 873 , 874 ]. Culturing the molluscs is a difficult-but-not-impossible task, which could also be useful for some heterobranch species.…”

Section: Discussionmentioning

confidence: 99%

Bioactive Compounds from Marine Heterobranchs

Ávila

Angulo‐Preckler

2020

Marine Drugs

View full text Add to dashboard Cite

The natural products of heterobranch molluscs display a huge variability both in structure and in their bioactivity. Despite the considerable lack of information, it can be observed from the recent literature that this group of animals possesses an astonishing arsenal of molecules from different origins that provide the molluscs with potent chemicals that are ecologically and pharmacologically relevant. In this review, we analyze the bioactivity of more than 450 compounds from ca. 400 species of heterobranch molluscs that are useful for the snails to protect themselves in different ways and/or that may be useful to us because of their pharmacological activities. Their ecological activities include predator avoidance, toxicity, antimicrobials, antifouling, trail-following and alarm pheromones, sunscreens and UV protection, tissue regeneration, and others. The most studied ecological activity is predation avoidance, followed by toxicity. Their pharmacological activities consist of cytotoxicity and antitumoral activity; antibiotic, antiparasitic, antiviral, and anti-inflammatory activity; and activity against neurodegenerative diseases and others. The most studied pharmacological activities are cytotoxicity and anticancer activities, followed by antibiotic activity. Overall, it can be observed that heterobranch molluscs are extremely interesting in regard to the study of marine natural products in terms of both chemical ecology and biotechnology studies, providing many leads for further detailed research in these fields in the near future.

show abstract

Section: Discussionmentioning

confidence: 99%

Bioactive Compounds from Marine Heterobranchs

Ávila

Angulo‐Preckler

2020

Marine Drugs

View full text Add to dashboard Cite

show abstract

“…Heterologous expression approaches of orphan gene clusters should be considered as an alternative strategy to tap the specific metabolome of these organisms. Molecular biological tools for such approaches are available and are undergoing a steady process of improvement 73 .…”

Section: Discussionmentioning

confidence: 99%

Analysis of the Genome and Metabolome of Marine Myxobacteria Reveals High Potential for Biosynthesis of Novel Specialized Metabolites

Moghaddam

Crüsemann

Alanjary

et al. 2018

Sci Rep

Self Cite

View full text Add to dashboard Cite

Comparative genomic/metabolomic analysis is a powerful tool to disclose the potential of microbes for the biosynthesis of novel specialized metabolites. In the group of marine myxobacteria only a limited number of isolated species and sequenced genomes is so far available. However, the few compounds isolated thereof so far show interesting bioactivities and even novel chemical scaffolds; thereby indicating a huge potential for natural product discovery. In this study, all marine myxobacteria with accessible genome data (n = 5), including Haliangium ochraceum DSM 14365, Plesiocystis pacifica DSM 14875, Enhygromyxa salina DSM 15201 and the two newly sequenced species Enhygromyxa salina SWB005 and SWB007, were analyzed. All of these accessible genomes are large (~10 Mb), with a relatively small core genome and many unique coding sequences in each strain. Genome analysis revealed a high variety of biosynthetic gene clusters (BGCs) between the strains and several resistance models and essential core genes indicated the potential to biosynthesize antimicrobial molecules. Polyketides (PKs) and terpenes represented the majority of predicted specialized metabolite BGCs and contributed to the highest share between the strains. BGCs coding for non-ribosomal peptides (NRPs), PK/NRP hybrids and ribosomally synthesized and post-translationally modified peptides (RiPPs) were mostly strain specific. These results were in line with the metabolomic analysis, which revealed a high diversity of the chemical features between the strains. Only 6–11% of the metabolome was shared between all the investigated strains, which correlates to the small core genome of these bacteria (13–16% of each genome). In addition, the compound enhygrolide A, known from E. salina SWB005, was detected for the first time and structurally elucidated from Enhygromyxa salina SWB006. The here acquired data corroborate that these microorganisms represent a most promising source for the detection of novel specialized metabolites.

show abstract

“…Hence, the isolation of compound-producing microorganisms might be an approach to solve the supply issue [ 198 ]. Even if the true producer is not yet culturable, a metagenomic toolbox is available to discover the biosynthetic gene cluster corresponding to the natural product of interest and using it for heterologous expression approaches [ 199 ]. Securing the supply will facilitate comprehensive analysis and testing in the lab, which is a precondition for translational research, once natural products enter drug development phase.…”

Section: Discussionmentioning

confidence: 99%

The Potential of Indonesian Heterobranchs Found around Bunaken Island for the Production of Bioactive Compounds

et al. 2017

View full text Add to dashboard Cite

The species diversity of marine heterobranch sea slugs found on field trips around Bunaken Island (North Sulawesi, Indonesia) and adjacent islands of the Bunaken National Marine Park forms the basis of this review. In a survey performed in 2015, 80 species from 23 families were collected, including 17 new species. Only three of these have been investigated previously in studies from Indonesia. Combining species diversity with a former study from 2003 reveals in total 140 species from this locality. The diversity of bioactive compounds known and yet to be discovered from these organisms is summarized and related to the producer if known or suspected (might it be down the food chain, de novo synthesised from the slug or an associated bacterium). Additionally, the collection of microorganisms for the discovery of natural products of pharmacological interest from this hotspot of biodiversity that is presented here contains more than 50 species that have never been investigated before in regard to bioactive secondary metabolites. This highlights the great potential of the sea slugs and the associated microorganisms for the discovery of natural products of pharmacological interest from this hotspot of biodiversity.

show abstract

Toolbox for Antibiotics Discovery from Microorganisms

Cited by 11 publications

References 78 publications

Bioactive Compounds from Marine Heterobranchs

Bioactive Compounds from Marine Heterobranchs

Analysis of the Genome and Metabolome of Marine Myxobacteria Reveals High Potential for Biosynthesis of Novel Specialized Metabolites

The Potential of Indonesian Heterobranchs Found around Bunaken Island for the Production of Bioactive Compounds

Contact Info

Product

Resources

About