Tools to Probe Nanoscale Surface Phenomena in Cellulose Thin Films: Applications in the Area of Adsorption and Friction

Song, Junlong; Li, Yan; Hinestroza, Juan P.; Rojas, Orlando J.

doi:10.1002/9781444307474.ch4

Cited by 6 publications

(3 citation statements)

References 100 publications

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“…The thickness of BSA was 5.2 and 4.8 nm, respectively, for before and after rinsing. Based on Sauerbrey estimation, all according values were less than half of the values based on viscoelastic modeling, indicating the layer formed was very soft and therefore the Sauerbrey estimation was substantially underestimated the layer thickness of cellulase and/or BSA [36].…”

Section: Resultsmentioning

confidence: 85%

Exploring the Promoting Mechanisms of Bovine Serum Albumin, Lignosulfonate, Polyethylene Glycol for Lignocellulose Saccharification from Perspective of Molecular Interactions with Cellulase

Wang

Liu

et al. 2021

Preprint

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Background: Bovine serum albumin (BSA), polyethylene glycol (PEG) and lignosulfonate (LS) have been extensively employed as synergistic agents in lignocellulose saccharification. Nevertheless, the promoting mechanisms have not been fully understood and there are a number of controversial opinions existed. All attention has been paid to the interactions between respective additive and substrate. However, rarely attention has been paid to the interactions between additives and cellulase. The interaction between respective additive and cellulase is actually more important since cellulase interacts with the additives before it contacts with substrate.Results: This investigation showed that BSA and LS could bind to cellulase to form complexes, whereas PEG did not. However, PEG had a high affinity to lignin or lignin derivatives. The complexes of Cell-BSA consisted of one BSA and four cellulase molecules; while the complexes of Cell-LS were composed of one cellulase and four LS molecules in the testing conditions. Regarding to the enzymatic hydrolytic efficiency for Avicel and lignocellulose substrates, the results showed that BSA and PEG promoted the enzymatic hydrolysis for both substrates, while LS had a promoting effect for lignocellulose only and inhibited some extent for Avicel. Conclusions: This study showed that synergistic agents of LS, BSA, and PEG have different interaction modes with cellulase. BSA and LS can form complexes with cellulase and the formed complexes prevent them from nonproductive binding by residue lignin; what’s more, the cellulase-BSA complexes improve the hydrolytic capability of pristine enzyme whereas cellulase-LS complexes reduce. The promoting mechanism of PEG has to be attributed to two aspects: one is to prevent cellulase from nonproductive binding to residue lignin by forming a thin layer that actually serves as steric hindrance on residue lignin; the other is the structure change of substrate induced by PEG addition. This investigation will help us to understand the sophisticated interactions among the components in the complicated enzymatic system, especially the interactions between enzymes and synergistic agents. It will be helpful in the design and utilization of synergistic additives in the lignocellulose biorefinery process as well.

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Section: Resultsmentioning

confidence: 85%

Exploring the Promoting Mechanisms of Bovine Serum Albumin, Lignosulfonate, Polyethylene Glycol for Lignocellulose Saccharification from Perspective of Molecular Interactions with Cellulase

Wang

Liu

et al. 2021

Preprint

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Section: Interaction Between Bsa and Cellulasementioning

confidence: 85%

Comparison of the Interaction Modes Between Cellulase and Bovine Serum Albumin /Lignosulfonate /Polyethylene Glycol in Promoting Lignocellulose Saccharification

Wang

Lan

Guo

et al. 2021

Preprint

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Background: Bovine serum albumin (BSA), polyethylene glycol (PEG) and lignosulfonate (LS) have been extensively employed as synergistic agents in lignocellulose saccharification, albeit it has not been fully understood how they interact with enzymes from the perspectives of molecular interactions. Herein, we attempted to unveil the promotion mechanisms of BSA, PEG and LS for lignocellulose saccharification from the perspective of their respective interaction with cellulase using Quartz Crystal Microbalance with Dissipation monitoring (QCM-D), Surface Plasmon Resonance (SPR), and Small Angle X-ray Scattering (SAXS) to investigate their respective interaction and the complex formation. In the meanwhile, we compared the effects of adding these additives into the enzymatic hydrolysis of pure cellulose (Avicel) and green liquor-pretreated lignocellulose (GL).Results: The results showed that BSA and LS could bind to cellulase to form complexes, whereas PEG did not. However, PEG had a high affinity to lignin or lignin derivatives. In term for Avicel and GL substrates, the results showed that BSA and PEG promoted the enzymatic hydrolysis of both substrates, while LS had a promoting effect for GL only and inhibited some extent for Avicel. Conclusions: This study showed that synergistic agents of LS, BSA, and PEG have different interaction modes with cellulase. BSA and LS form complexes with cellulase and the formed complexes prevent from nonproductive binding by residue lignin; whereas PEG prevents from nonproductive binding by forming a thin layer on residue lignin which actually serve as steric hindrance. This investigation will help us to understand the sophisticated interactions among the components in the complicated enzymatic system, especially the interactions between enzymes and synergistic agents. It will be helpful in the design and utilization of synergistic additives in the lignocellulose biorefinery process as well.

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“…Adsorption of AmPAM on various substrates was conducted on a Quartz Crystal Microbalance with Dissipation mode, E4 model (Biolin Scientific Co. Ltd., Gothenburg, Sweden). The principles of this technique can be found in [39].…”

Section: Methodsmentioning

confidence: 99%

Revealing Adsorption Behaviors of Amphoteric Polyacrylamide on Cellulose Fibers and Impact on Dry Strength of Fiber Networks

et al. 2019

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Amphoteric polyacrylamide (AmPAM) has been widely used in a variety of industrial areas and the adsorption behavior of AmPAM plays a crucial role in its applications. In this study, a series of AmPAMs with various molecular weights (MW) were synthesized; and their impact on dry strength of fiber networks or paper was assessed. The results showed that the optimal MW of AmPAM for strength enhancement ranged between 300 and 500 k. More importantly, the adsorption behaviors of three typical AmPAM samples on silica (model substrate) and cellulose surfaces were revealed using a quartz crystal microbalance with dissipation monitoring (QCM-D) in situ and in real time. The adsorption dynamics of AmPAM and the conformation of the adlayers were further derived. The results indicated that a relatively high adsorption amount was achieved under the conditions of a high polymer concentration, a medium pH close to its isoelectric point (IEP), a mild ionic strength, and a high charged surface; whereas the MW of AmPAM had little effect on the equilibrium adsorption mass of AmPAM, but significantly affected the conformation of adsorbed layer on substrates. Based on the adsorption behaviors of AmPAM, the explanation of the best dry strength achieved in a narrow range of MW of AmPAM is proposed. It was concluded that the appropriate balance between bridging and flocculation, penetration into fiber pores, and conformation were only achieved in the optimal MW range of AmPAM. The findings obtained from in this work enable us to better understand the adsorption behaviors of polyampholyte, and provide a guideline on molecular design of AmPAM and its applications from both fundamental and practical points of view.

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Tools to Probe Nanoscale Surface Phenomena in Cellulose Thin Films: Applications in the Area of Adsorption and Friction

Cited by 6 publications

References 100 publications

Exploring the Promoting Mechanisms of Bovine Serum Albumin, Lignosulfonate, Polyethylene Glycol for Lignocellulose Saccharification from Perspective of Molecular Interactions with Cellulase

Exploring the Promoting Mechanisms of Bovine Serum Albumin, Lignosulfonate, Polyethylene Glycol for Lignocellulose Saccharification from Perspective of Molecular Interactions with Cellulase

Comparison of the Interaction Modes Between Cellulase and Bovine Serum Albumin /Lignosulfonate /Polyethylene Glycol in Promoting Lignocellulose Saccharification

Revealing Adsorption Behaviors of Amphoteric Polyacrylamide on Cellulose Fibers and Impact on Dry Strength of Fiber Networks

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