Tooth Tissue Engineering: Optimal Dental Stem Cell Harvest Based on Tooth Development

Abstract

Our long-term objective is to devise reliable methods to generate biological replacement teeth exhibiting the physical properties and functions of naturally formed human teeth. Previously, we demonstrated the successful use of tissue engineering approaches to generate small, bioengineered tooth crowns from harvested pig and rat postnatal dental stem cells (DSCs). To facilitate characterizations of human DSCs, we have developed a novel radiographic staging system to accurately correlate human third molar tooth developmental stage with anticipated harvested DSC yield. Our results demonstrated that DSC yields were higher in less developed teeth (Stages 1 and 2), and lower in more developed teeth (Stages 3, 4, and 5). The greatest cell yields and colony-forming units (CFUs) capability was obtained from Stages 1 and 2 tooth dental pulp. We conclude that radiographic developmental staging can be used to accurately assess the utility of harvested human teeth for future dental tissue engineering applications.

Tooth Tissue Engineering: The Influence of Hydrophilic Surface on Nanocrystalline Diamond Films for Human Dental Stem Cells

“…23 Tooth bud cell yields from impacted third molar teeth varied depending on the age of the individual and developmental stage of the tooth. 9 Teeth less developed extracted usually are present in younger individuals. These were also less mineralized structures and provided more cells due to the presence of more soft tissue; this kept the stemness ability detected on the analysis of colony-forming units in early tooth development stage while other more developed teeth had more mineralized structures and less soft tissue quantity.…”

“…We obtained on average cell yields of *1.0 · 10 6 cell/impacted third molar tooth. 9 SEM images of ECM deposited by hDSCs grown on Hand O-terminated diamond surfaces are shown (Fig. 5).…”

“…[2][3][4][5][6][7][8][9] Strategies for TTE, as first described by Langer and Vacanti 10 and Kaigler and Mooney, 1 can be classified into three typesconductive, inductive, and cellular transplantation-the latter of which has guided the approach taken by this group for TTE.…”

Tooth Tissue Engineering: The Importance of Blood Products as a Supplement in Tissue Culture Medium for Human Pulp Dental Stem Cells

“…Extracted tooth germs, tooth development stage 2, 11 were immediately placed in Hank's balanced salt solution (HBSS; Gibco BRL, Gaithersburg, MD, USA) at 37°C and transported to the laboratory. The pulp tissue was removed, weighed, and broken up into pieces smaller than 1 mm in fresh HBSS, washed, and digested for 30-40 min with 0.4 mg/mL of type II collagenase (Sigma-Aldrich) and 0.2 mg/mL dispase I (Boehringer Mannheim, Indianapolis, IN).…”

Effects of glucose and glutamine concentrations in human dental pulp stem cells viability. An approach for cell transplantation

“…Human dental pulp stem cells (DPSc) have a great potential for Regenerative Medicine (RM) applications 1,2 . However, a preclinical phase still needs in vitro steps for cell proliferation, and well-established patterns are required to guarantee the conception of an appropriate biological material to restore human parts [3][4][5][6] .…”