Top‐sprayed phytase enhances the digestibility of energy, protein, amino acids and minerals, and reduces phosphorus output in Nile tilapia fed all‐vegetable diets

Pontes, Tânia Cristina; Brito, Johnny Martins de; Wernick, Bruno; Urbich, Allan Vinnícius; Panaczevicz, Paola Aparecida Paulovski; Miranda, João Antônio Galiotto; Furuya, Valéria Rossetto Barriviera; Furuya, Wilson Massamitu

doi:10.1111/are.15527

Cited by 12 publications

(12 citation statements)

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“…Reports showed that this microbial phytase is safe for use in avian and porcine nutrition (Dersjant‐Li et al, 2017; Ladics et al, 2020) and can efficiently promote their overall growth performance (Chen et al, 2019; Dersjant‐Li & Kwakernaak, 2019; Rychen et al, 2017). Previous studies evaluated the effects of different sources of phytase on growth, P contents, digestibility of nutrients, minerals and blood profiles of several fish species (Akpoilih et al, 2016; Akpoilih et al, 2017; Baruah et al, 2007; Chen et al, 2019; Hussain et al, 2011; Liebert & Portz, 2005; Nwanna & Schwarz, 2007), as well as P balance/retention (Pontes et al, 2021); bone, whole body and plasma mineral composition (Akpoilih et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Effects of dietary supplementation with microbial phytase on the growth, bone minerals, antioxidant status, innate immunity and disease resistance of African catfish fed on high soybean meal‐based diets

Adeshina

Akpoilih

Tiamiyu

et al. 2022

Animal Physiology Nutrition

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The microbial phytase, derived from Buttiauxella gaviniae, Yersinia mollarettiv and Hafnia spp., is proven to be safe for avian and porcine feeding and promotes their overall growth performance. Here, we have evaluated microbial phytase's effects on the growth, bone mineral content, antioxidant status, immune responses and the resistance of African catfish (Clarias gariepinus) fed with high soybean meal‐based diets against Aeromonas hydrophila infection. Five isonitrogenous diets (40% protein) were supplemented with different levels of microbial phytase ranging from 0 as a control to 250, 500, 750 and 1000 FTU/kg diet. African catfish (n = 300; 8.5 ± 0.3 g) were allocated in 15 50‐L tanks (in triplicates) and were fed on the prepared tested diets for 12 weeks. After the end of the feeding period of 12 weeks, 10 fish from each replicate was intraperitoneally infected with A. hydrophila (0.5 × 105 CFU/ml) and monitored for 14 days. Dietary phytase levels linearly and quadratically improved the growth performance of African catfish and stimulated feed intake. Bone levels of calcium, phosphorus, magnesium and zinc were also positively modulated in phytase‐fed fish, especially at 750–1000 FTU/kg diet. Similarly, counts of red and white blood cells as well as haemoglobin, packed cells volume, platelets, lymphocytes and heterocytes were significantly modulated in all fish fed with phytase‐supplemented diets. Higher levels of serum total protein, albumin and globulin were also observed in fish fed with a 750–1000 FTU/kg diet of phytase. Conversely, aspartate and alanine aminotransferase activities were lower in fish fed with a 750–1000 FTU/kg diet of phytase, compared to those fed the control diet. Moreover, antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase), reduced glutathione levels and immune responses (lysozyme, respiratory burst, protease and alkaline phosphatase activities) biomarkers were linearly and quadratically elevated, while malondialdehyde values were linearly and quadratically decreased in fish groups fed with phytase‐based diets. After administering A. hydrophila, 60.0% of the fish fed the control diet perished, while no mortalities were observed in fish fed with 750–1000 FTU/kg diets. Taken together, the current study reveals that dietary phytase could improve the growth performance, blood profile, bone mineralization, antioxidant activities, immunity and overall protection of African catfish against A. hydrophila infection. Dietary phytase may be efficiently used in the feeding of African catfish to enhance their overall performance and mitigate health conditions with optimum level of 900 FTU/kg diet.

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Section: Introductionmentioning

confidence: 99%

Effects of dietary supplementation with microbial phytase on the growth, bone minerals, antioxidant status, innate immunity and disease resistance of African catfish fed on high soybean meal‐based diets

Adeshina

Akpoilih

Tiamiyu

et al. 2022

Animal Physiology Nutrition

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“…The control diet contained 5 g kg −1 of Cr 2 O 3 as an indigestible marker (Table 1). We assumed that all‐vegetable containing blend of XB (1120 TXU kg −1 diet of 1,4‐β‐xylanase and 500 TGU kg −1 diet of 1,4‐β‐glucanase) enhances the digestible energy content by 0.41 MJ kg −1 diet (Brito et al, 2021), and phytase at 1000 FTU kg −1 diet increases the available P content by 4.5 g kg −1 (Pontes et al, 2021). These assumptions were based on those mentioned above‐published research conducted in our laboratory to determine the optimum levels of these exogenous enzymes supplemented in all‐vegetable diet fed to Nile tilapia.…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, vegetable ingredients, such as wheat bran, could be applicable in large‐scale aquafeed production (Ghosh et al, 2019). Recent research studies conducted in our laboratory have proved that individual phytase and a mixture of xylanase and β‐glucanase can improve the nutritive value of the all‐vegetable diet fed to Nile tilapia (Brito et al, 2021; Pontes et al, 2021). Nevertheless, based on the author's knowledge, the effects of the exogenous phytase and xylanase and β‐glucanase preparation enzymes individually or in combination with increasing digestible energy, digestible protein and available P contents in all‐vegetable diets fed to Nile tilapia are yet to be investigated.…”

Section: Introductionmentioning

confidence: 99%

Top‐sprayed mixture of phytase, xylanase and β‐glucanase enhances energy and nutrient matrix in all‐vegetable diets for Nile tilapia

Nakamura

Macêdo

Cruz

et al. 2022

Aquaculture Research

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This study aimed to evaluate the effects of supplemental phytase with xylanase and β‐glucanase blend on the apparent digestibility coefficient (ADC) of gross energy and nutrients, including amino acids, minerals, and nitrogen (N) and phosphorus (P) balance in Nile tilapia, Oreochromis niloticus. Diets were elaborated either without enzyme supplementation (control) or supplemented with phytase (PHY), xylanase and β‐glucanase (XB) blend or a combination of phytase plus xylanase and β‐glucanase (PHY+XB) blend. Fish (n = 192; 60 ± 3.4 g) were fed with the experimental diets replicated four times. Compared with the control group, fish‐fed with the PHY+XB diet showed a higher ADC of gross energy and nutrients, including amino acids and minerals. Also, compared to control diet, digestible energy, digestible protein and available P contents were higher in fish‐fed diet PHY+XB by 1.63 MJ kg−1, 7.21 and 4.70 g kg−1 diet respectively. Compared to the control group, fish‐fed diet PHY+XB displayed lower total N and P loss of 17.51 and 10.09 g kg−1 of body weight gain (BWG) respectively. The study findings provided new insight into the nutritive value and ecofriendliness/sustainability of diets associated with exogenous enzymes for tilapia culture.

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“…Whereas, at the alkaline pH of intestine, the phytate–protein association makes a ternary complex with cations, restricting protein digestibility 42 . There is no direct effect of phytic acid on the digestibility of protein, rather it is a consequence of amino acid digestibility affected due to enhancement in loss of endogenous amino acid in the intestine 27 . The digestibility of protein and the growth of rainbow trout ( Oncorhynchus mykiss ) remarkably decreased on the supplementation of feed with 0.5% phytate, however, feed containing 0.8% phytate though affects protein digestibility of S .…”

Section: Impact Of Phytate On Growth and Digestive Enzymes Of Fishmentioning

confidence: 99%

“…On a commercial scale, microbes can be easily propagated, and the phytase produce by them is stable and usable under different aquaculture conditions 25 . Over the last decades, considerable progress has been made to understand the effect of microbial phytases as feed additive and their impact on fish growth performance 23,26,27 . The role of phytases in improving the growth performances and nutrition in aquaculture has been documented earlier 23,28–30 .…”

Section: Introductionmentioning

confidence: 99%

Role of microbial phytases in improving fish health

Priya

Virmani

Pragya

et al. 2023

Reviews in Aquaculture

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Fishmeal is the most preferred protein source in aquafeed industry due to its balanced amino acid and fatty acid profile, but its limited global supply and high price value, necessitates researchers to focus on sustainable alternative sources of protein and plant‐based feed, to meet the growing demand of fish feed. Phytate is a reservoir of phosphorus present in the plants; however, its chelating ability makes it anti‐nutritional. Phytate forms complexes with minerals, proteins, lipids and carbohydrates, confining their accessibility in fish feeds, hence, additional dietary nutrients are required in the fish feed to overcome nutritional deficiencies. The elimination of phytate‐phosphorus through animal faeces and its pass on to aquatic bodies leads to enrichment of nutrients, eutrophication and algal blooms. Microbial phytase segregates phosphorus, minerals, proteins and lower inositol phosphates from phytate. The supplementation of fish feed with microbial phytase enhances the digestibility of minerals, due to which less nutrients are excreted in the faeces; therefore, minimizing pollution in water bodies. Monogastric and agastric animals inherently exhibit the deficiency of phytase; hence, supplementation of dietary material with phytase has been an emerging choice to make dietary nutrients available for fish. Moreover, microbial phytases have a broad pH range and are thermostable, which makes them an excellent choice for commercial fish feed production. Additionally, microbial phytase improves fish growth as these enzymes enhance nutrients bioavailability and their digestion. This review article presents the application of microbial phytases as a feed additive, and their role in enhancing fish growth.

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Top‐sprayed phytase enhances the digestibility of energy, protein, amino acids and minerals, and reduces phosphorus output in Nile tilapia fed all‐vegetable diets

Cited by 12 publications

References 35 publications

Effects of dietary supplementation with microbial phytase on the growth, bone minerals, antioxidant status, innate immunity and disease resistance of African catfish fed on high soybean meal‐based diets

Effects of dietary supplementation with microbial phytase on the growth, bone minerals, antioxidant status, innate immunity and disease resistance of African catfish fed on high soybean meal‐based diets

Top‐sprayed mixture of phytase, xylanase and β‐glucanase enhances energy and nutrient matrix in all‐vegetable diets for Nile tilapia

Role of microbial phytases in improving fish health

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