Topical Application of Selenium Can Significantly Relieve UV-induced Skin Aging in Hairless Mice

Abstract

Ultraviolet (UV) irradiation is an environmental factor that causes skin aging, and is also a major factor leading to cumulative alterations of skin structure, function and appearance. To investigate the effects of Selenium (Sel) on UV-induced skin aging, hairless mice were treated for 4 weeks with UV irradiation and topical application of Sel. Then, the effects of Sel were measured in the skin of these mice via histological analysis and Western blotting. According to the results of wrinkle formation analysis, the topical application of Sel induced a reduction in wrinkling formation in the damaged skin of the UV-irradiated mice. Additionally, our histological analysis demonstrated that the skin thickness in the Sel-treated group was less than in the UV-irradiated group. Furthermore, in an effort to investigate the mechanisms underlying the effects of Sel, the expression levels of matrix-metalloproteinase (MMP) and MAPK protein were assessed in both groups. The application of Sel induced a reduction in MMP-1 expression levels to the levels observed in the non-irradiated group. However, the expression level of MMP-9 was increased slightly in the Sel application group as compared with the vehicle application group. Additionally, the levels of ERK phosphorylation were increased by the application of Sel, but the levels of JNK and p38 were not altered by Sel treatment. These results suggest the possibility that Sel should be considered as a skin aging-protective and therapeutic drug candidate, which functions via the regulation of MMP expression levels.

UV radiation-induced skin aging in hairless mice is effectively prevented by oral intake of sea buckthorn (Hippophae rhamnoides L.) fruit blend for 6 weeks through MMP suppression and increase of SOD activity

“…UV radiation. The minimal erythemal dose (MED) from the UV irradiation device was determined using the procedures suggested in previous studies (16,17). A UV irradiation device was made from a TL20W/12RS UV lamp and Kodacel filter in a rectangular parallelepiped box.…”

“…Evaluation of wrinkle formation. Wrinkle formation was measured using the procedure established by our laboratory using a DETAX System II (MIXPAC) and Double-Stick Disc (3M Health Care, Germany) (17). After 6 weeks, skin surface impressions (replica) were prepared by applying silicon rubber in mixed liquid form secreted from a DETAX System II to the dorsal skin of the mice.…”

Protective effects of the antioxidant extract collected from Styela clava tunics on UV radiation-induced skin aging in hairless mice

“…UV radiation and topical administration. The minimal erythemal dose (MED) from the UV irradiation device was determined as suggested in previous studies (21)(22)(23). Briefly, a UV irradiation device was made from a TL20W/12RS UV lamp and a Kodacel filter in a rectangular parallelepiped box.…”