Topical Cryopreserved Amniotic Membrane and Umbilical Cord Eye Drops Promote Re-Epithelialization in a Murine Corneal Abrasion Model

Tighe, Sean; Moein, Hamid-Reza; Chua, Lorraine; Cheng, Anny M. S.; Hamrah, Pedram; Tseng, Scheffer C.G.

doi:10.1167/iovs.16-20834

Cited by 25 publications

(41 citation statements)

References 36 publications

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“…Theirregularityofcornealepitheliumwasgradedbyusinga5-point scalebasedonthenumberofdistortedquartersinthereflectedring as described previously. 36,37 In general, corneal epithelial regularity wascomparableinallgroupsbeforeabrasion,notablydistorted1day Consistent with previous studies, [26][27][28]…”

Section: Corneal Epithelial Regularity After CXLsupporting

confidence: 88%

“…The irregularity of corneal epithelium was graded by using a 5‐point scale based on the number of distorted quarters in the reflected ring as described previously 36, 37. In general, corneal epithelial regularity was comparable in all groups before abrasion, notably distorted 1 day after abrasion in all groups, and less distorted in the 9‐minutes CXL–treated group ( P < .05) but progressively improved 2 days after corneal abrasion with no significant difference between the CXL group and the control group (Figure 8).…”

Section: Resultsmentioning

confidence: 99%

“…A 5-point scale was usedtoassesstheirregularityofthecornealsurfaceaspreviously described. 36,37 This result indicates that corneal CXL is a novel method to reduceleukocytesininflamedcorneas.…”

Section: Flow Cytometrymentioning

confidence: 86%

“…A 5‐point scale was used to assess the irregularity of the corneal surface as previously described 36, 37…”

Section: Methodsmentioning

confidence: 99%

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UV light crosslinking regresses mature corneal blood and lymphatic vessels and promotes subsequent high-risk corneal transplant survival

Hou

Tóth

et al. 2018

American Journal of Transplantation

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Immunologic graft rejection is the main complication after corneal transplant into pathologically prevascularized so‐called high‐risk eyes. The aim of this study was to evaluate whether ultraviolet (UV) light crosslinking can regress pathologic corneal blood and lymphatic vessels and thereby improve subsequent graft survival. Using the murine model of suture‐induced corneal neovascularization, we found that corneal crosslinking with UVA light and riboflavin regressed both preexisting blood and lymphatic vessels significantly via induction of apoptosis in vascular endothelial cells. In addition, macrophages and CD45+ cell counts were significantly reduced. Consistently, corneal crosslinking reduced keratocyte density and corneal thickness without affecting corneal nonvascular endothelial cells, iris, and lens depending on the crosslinking duration. Furthermore, using the murine model of corneal transplant, long‐term graft survival was significantly promoted (P < .05) and CD4+ CD25+FoxP3+ T regulatory cells were upregulated (P < .01) in high‐risk eyes preoperatively treated with crosslinking. Our results suggest UV light crosslinking as a novel method to regress both pathologic corneal blood and lymphatic vessels and to reduce CD45+ inflammatory cells. Furthermore, this study demonstrates for the first time that preoperative corneal crosslinking in prevascularized high‐risk eyes can significantly improve subsequent graft survival and may become a promising novel therapy in the clinic.

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Section: Corneal Epithelial Regularity After CXLsupporting

confidence: 88%

Section: Resultsmentioning

confidence: 99%

Section: Flow Cytometrymentioning

confidence: 86%

“…A 5‐point scale was used to assess the irregularity of the corneal surface as previously described 36, 37…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

UV light crosslinking regresses mature corneal blood and lymphatic vessels and promotes subsequent high-risk corneal transplant survival

Hou

Tóth

et al. 2018

American Journal of Transplantation

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“…For example, mesenchymal stem cells (MSCs) derived from cord blood and Wharton's jelly are reported to be immunologically privileged and contain anti‐fibrotic characteristics that may promote scarless wound healing . Preliminary studies have shown that a cryopreserved umbilical cord product has been used as a graft to treat complex foot ulcers and can promote re‐epithelialization in a murine corneal abrasion model, indicating that the tissue of the umbilical cord has therapeutic potential in promoting soft tissue healing. However, it has yet to be determined whether these properties and biological signals also exist in a dehydrated form of the umbilical cord matrix as well.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of dehydrated human umbilical cord biological properties for wound care and soft tissue healing

Bullard¹,

Lei²,

Lim³

et al. 2018

J Biomed Mater Res

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Chronic wounds are a significant health care problem with serious implications for quality of life because they do not properly heal and often require therapeutic intervention. Amniotic membrane allografts have been successfully used as a biologic therapy to promote soft tissue healing; however, the umbilical cord, another placental-derived tissue, has also recently garnered interest because of its unique composition but similar placental tissue origin. The aim of this study was to characterize PURION® PLUS Processed dehydrated human umbilical cord (dHUC) and evaluate the biological properties of this tissue that contribute to healing. This was performed through the characterization of the tissue composition, evaluation of in vitro cellular response to dHUC treatment, and in vivo bioresorption and tissue response in a rat model. It was observed that dHUC contains collagen I, hyaluronic acid, laminin, and fibronectin. Additionally, 461 proteins that consist of growth factors and cytokines, inflammatory modulators, chemokines, proteases and inhibitors, adhesion molecules, signaling receptors, membrane-bound proteins, and other soluble regulators were detected. Cell-based assays demonstrated an increase in adipose-derived stem cell and mesenchymal stem cell proliferation, fibroblast migration and endothelial progenitor cell vessel formation in a dose-dependent manner after dHUC treatment. Lastly, rat subcutaneous implantation demonstrated biocompatibility since dHUC allografts were resorbed without fibrous encapsulation. These findings establish that dHUC possesses biological properties that stimulate cellular responses important for soft tissue healing. © 2018 The Authors. Journal Of Biomedical Materials Research Part B: Applied Biomaterials Published By Wiley Periodicals, Inc. J Biomed Mater Res B Part B: Appl Biomater, 2018.

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Corneal Trauma

Xu,

Huang

2024

Ocular Trauma

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Topical Cryopreserved Amniotic Membrane and Umbilical Cord Eye Drops Promote Re-Epithelialization in a Murine Corneal Abrasion Model

Cited by 25 publications

References 36 publications

UV light crosslinking regresses mature corneal blood and lymphatic vessels and promotes subsequent high-risk corneal transplant survival

UV light crosslinking regresses mature corneal blood and lymphatic vessels and promotes subsequent high-risk corneal transplant survival

Evaluation of dehydrated human umbilical cord biological properties for wound care and soft tissue healing

Corneal Trauma

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