Topographically modified surfaces affect orientation and growth of hippocampal neurons

Dowell-Mesfin, Natalie; Abdul-Karim, M. A.; Turner, A. M.P.; Schanz, S.; Craighead, Harold G.; Roysam, Badrinath; Turner, James N.; Shain, William

doi:10.1088/1741-2560/1/2/003

Cited by 209 publications

(238 citation statements)

References 50 publications

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“…Indirect patterning of the silicone surface as generated by molding guided neurites of both cell types in parallel to the grooves, as also observed earlier in various other studies on PC-12 cells 30,31 and primary isolated neuronal cells such as CNS neuroblasts 25 and hippocampal cells. 35 However, in our study microstructures generated by direct laser ablation did not influence the direction of neurite outgrowth in either of the two cell types. When envisioning the manufacturing process by direct laser ablation two major reasons may account for this finding: (i) the quality of the generated structure and (ii) the chemical properties.…”

Section: Discussioncontrasting

confidence: 64%

Directing neuronal cell growth on implant material surfaces by microstructuring

et al. 2012

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CitationDirecting neuronal cell growth on implant material surfaces by microstructuring. 2012, 100 (4) Abstract: For best hearing sensation, electrodes of auditory prosthesis must have an optimal electrical contact to the respective neuronal cells. To improve the electrode-nerve interface, microstructuring of implant surfaces could guide neuronal cells toward the electrode contact. To this end, femtosecond laser ablation was used to generate linear microgrooves on the two currently relevant cochlear implant materials, silicone elastomer and platinum. Silicone surfaces were structured by two different methods, either directly, by laser ablation or indirectly, by imprinting using laser-microstructured molds. The influence of surface structuring on neurite outgrowth was investigated utilizing a neuronal-like cell line and primary auditory neurons. The pheochromocytoma cell line PC-12 and primary spiral ganglion cells were cultured on microstructured auditory implant materials. The orientation of neurite outgrowth relative to the microgrooves was determined. Both cell types showed a preferred orientation in parallel to the microstructures on both, platinum and on molded silicone elastomer. Interestingly, microstructures generated by direct laser ablation of silicone did not influence the orientation of either cell type. This shows that differences in the manufacturing procedures can affect the ability of microstructured implant surfaces to guide the growth of neurites. This is of particular importance for clinical applications, since the molding technique represents a reproducible, economic, and commercially feasible manufacturing procedure for the microstructured silicone surfaces of medical implants. V C 2012 Wiley Periodicals, Inc.J Biomed Mater Res Part B: Appl Biomater 00B: 000-000, 2012.

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Section: Discussioncontrasting

confidence: 64%

Directing neuronal cell growth on implant material surfaces by microstructuring

et al. 2012

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“…A common feature observed on these pillared surfaces is a neurite channeling effect between pillars when the inter-pillar spacing is larger but close to the neurite width (≈ 1-2µm) [4][5][6] .Another remarkable effect is the accelerated neurite elongation provided by micro-pillars 4,6 . Interestingly, axons have been reported to specify preferentially within micro-pillars areas compared to control flat surfaces 6 , a feature also observed when micro-pillars are replaced by submicroscale holes 7 .…”

Section: Introductionmentioning

confidence: 96%

Nanoscale Surface Topography Reshapes Neuronal Growth in Culture

et al. 2014

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Neurons are sensitive to topographical cues provided either by in vivo or in vitro environments on the micrometric scale. We have explored the role of randomly distributed silicon nano-pillars on primary hippocampal neurite elongation and axonal differentiation. We observed that neurons adhere on the upper part of nano-pillars with a typical distance between adhesion points of about 500nm. These neurons produce less neurites, elongate faster, and differentiate an axon earlier than those grown on flat silicon surfaces. Moreover, when confronted to a differential surface topography, neurons specify an axon preferentially onto nano-pillars. As a whole, these results highlight the influence of the physical environment in many aspects of neuronal growth.

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“…2,5,14,15 The same occurs in vivo because the extracellular matrix (ECM) provides biophysical cues with its 3D morphology. 16 These effects have been largely explored using neuronal cells in order to realize an optimal micro/nanometrical environment able not only to guide mature neuronal growth 17 and dendrite orientation 18 but also to drive neuronal differentiation. 5,19,20 The effect of substrate topography has been investigated at the micrometrical and nanometrical scale.…”

Section: Introductionmentioning

confidence: 99%

A nanoporous surface is essential for glomerular podocyte differentiation in three-dimensional culture

Zennaro¹,

Rastaldi²,

Bakeine³

et al. 2016

IJN

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Although it is well recognized that cell–matrix interactions are based on both molecular and geometrical characteristics, the relationship between specific cell types and the three-dimensional morphology of the surface to which they are attached is poorly understood. This is particularly true for glomerular podocytes – the gatekeepers of glomerular filtration – which completely enwrap the glomerular basement membrane with their primary and secondary ramifications. Nanotechnologies produce biocompatible materials which offer the possibility to build substrates which differ only by topology in order to mimic the spatial organization of diverse basement membranes. With this in mind, we produced and utilized rough and porous surfaces obtained from silicon to analyze the behavior of two diverse ramified cells: glomerular podocytes and a neuronal cell line used as a control. Proper differentiation and development of ramifications of both cell types was largely influenced by topographical characteristics. Confirming previous data, the neuronal cell line acquired features of maturation on rough nanosurfaces. In contrast, podocytes developed and matured preferentially on nanoporous surfaces provided with grooves, as shown by the organization of the actin cytoskeleton stress fibers and the proper development of vinculin-positive focal adhesions. On the basis of these findings, we suggest that in vitro studies regarding podocyte attachment to the glomerular basement membrane should take into account the geometrical properties of the surface on which the tests are conducted because physiological cellular activity depends on the three-dimensional microenvironment.

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Topographically modified surfaces affect orientation and growth of hippocampal neurons

Cited by 209 publications

References 50 publications

Directing neuronal cell growth on implant material surfaces by microstructuring

Directing neuronal cell growth on implant material surfaces by microstructuring

Nanoscale Surface Topography Reshapes Neuronal Growth in Culture

A nanoporous surface is essential for glomerular podocyte differentiation in three-dimensional culture

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