“…The possible interaction of drFtsK with other deinococcal proteins of cell division like FtsZ, FtsA, and DivIVA, and genome segregation proteins like TopoIB, ParB2, ParB3, and ParB4 was monitored in surrogate E. coli by using the co-immunoprecipitation method. The recombinant pUT18 plasmids were already used in the earlier studies (Maurya et al, 2016, Maurya et al, 2018, Chaudhary et al, 2019, Kota et al, 2021) were transformed in E. coli BL21 (DE3) pLysS strain expressing Histidine tagged FtsK (pETFtsK). E. coli cells co-expressing histidine-tagged FtsK with T18-tagged protein in separate combinations were obtained at the log phase and cell-free extracts were prepared.…”