Toscana Virus Isolated from Sandflies, Tunisia

Lau, Colleen L.; Dachraoui, Khalil; Piorkowski, Géraldine; Chelbi, Ifhem; Moureau, Grégory; Cherni, Saifedine; Lamballerie, Xavier De; Sakhria, Sonia; Charrel, Rémi N.; Zhioua, Elyes

doi:10.3201/eid1902.121463

Cited by 39 publications

(46 citation statements)

References 10 publications

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“…A similar infection rate was observed in Turkey for ADAV (0.01 %) (Alkan et al, 2015). The MVV infection rate is of the same order of magnitude as those for TOSV in Tunisia (0.03 %) and in Spain (0.05 %) (Sanbonmatsu-Gámez et al, 2005;Bichaud et al, 2013). To increase the chances of viral isolation, live sandflies were not identified to species level because identification of individual specimens is time-consuming.…”

Section: Microneutralization Assaysmentioning

confidence: 62%

Isolation, full genomic characterization and neutralization-based human seroprevalence of Medjerda Valley virus, a novel sandfly-borne phlebovirus belonging to the Salehabad virus complex in northern Tunisia

Bichaud

Dachraoui

Alwassouf

et al. 2016

Journal of General Virology

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A new phlebovirus, Medjerda Valley virus (MVV), was isolated from one pool of Phlebotomus sp. (Diptera; Psychodidae) sandflies trapped in the vicinity of the Utique site, northern Tunisia. Genetic analysis based on complete coding of genomic sequences of the three RNA segments indicated that MVV is most closely related to members of the Salehabad virus species, where it is the fourth virus for which the complete sequence is available. A seroprevalence study was performed to search for neutralizing antibodies in human sera in the same region. The results demonstrate that in this area, MVV can readily infect humans despite low seroprevalence rates. Salehabad species viruses have generally been considered to be a group of viruses with little medical or veterinary interest. This view deserves to be revisited according to our human seroprevalence results, together with high animal infection rate of Adana virus and recent evidence of human infection with Adria virus in Greece. Further studies are needed to investigate the capacity of each specific member of the Salehabad virus species to cause human or animal diseases.

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Section: Microneutralization Assaysmentioning

confidence: 62%

Isolation, full genomic characterization and neutralization-based human seroprevalence of Medjerda Valley virus, a novel sandfly-borne phlebovirus belonging to the Salehabad virus complex in northern Tunisia

Bichaud

Dachraoui

Alwassouf

et al. 2016

Journal of General Virology

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“…It was confirmed by using two real-time RT-PCR assays specifically designed for ADAV. First, although lower than in other studies, the ADAV infection rate is in the same order of magnitude as that of Toscana virus in Tunisia (0.03%) and in Spain (0.05%) (52,53). Second, this study is the first one to calculate a rate of infection for a phlebovirus belonging to the Salehabad virus species.…”

Section: Discussionmentioning

confidence: 97%

Isolation, Genetic Characterization, and Seroprevalence of Adana Virus, a Novel Phlebovirus Belonging to the Salehabad Virus Complex, in Turkey

Alkan

Alwassouf

Piorkowski

et al. 2015

J Virol

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A new phlebovirus, Adana virus, was isolated from a pool of Phlebotomus spp. (Diptera; Psychodidae) in the province of Adana, in the Mediterranean region of Turkey. Genetic analysis based on complete coding of genomic sequences indicated that Adana virus belongs to the Salehabad virus species of the genus Phlebovirus in the family Bunyaviridae. Adana virus is the third virus of the Salehabad virus species for which the complete sequence has been determined. To understand the epidemiology of Adana virus, a seroprevalence study using microneutralization assay was performed to detect the presence of specific antibodies in human and domestic animal sera collected in Adana as well as Mersin province, located 147 km west of Adana. The results demonstrate that the virus is present in both provinces. High seroprevalence rates in goats, sheep, and dogs support intensive exposure to Adana virus in the region, which has not been previously reported for any virus included in the Salehabad serocomplex; however, low seroprevalence rates in humans suggest that Adana virus is not likely to constitute an important public health problem in exposed human populations, but this deserves further studies. IMPORTANCE Until recently, in the genus

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“…8,9 In addition, sand flies taken from pools in northern Tunisia have been shown to carry strains of TOSV. 10 The TOSV infection can be diagnosed using direct Page 4 of 20 A c c e p t e d M a n u s c r i p t methods, such as virus isolation, vital antigen detection by immunofluorescence, and RT PCR, or by indirect methods, detecting the presence of specific IgM and IgG antibodies using immunoenzymatic techniques. 11 The main objective of this study was to assess the prevalence of IgG antibodies against TOSV in Tunisia by ELISA test and to determine the extent of its circulation in the country.…”

Section: Introductionmentioning

confidence: 99%