Total and allergen‐specific immunoglobulin E in the serum and nasal mucosa of a nonallergic population

Reisacher, William R.

doi:10.1002/alr.21709

Cited by 8 publications

(5 citation statements)

References 21 publications

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“…We found no association between cat allergy and SPT or serum sIgE. In a recent study, 95% of participants had nasal sIgE above a 0.10 kU/L cutoff, but only 40% had serum levels above this cutoff 8 . Similarly, Zhang et al 9 .…”

Section: Discussionsupporting

confidence: 46%

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Nasal mucosal brush biopsy for cat allergy

Davison

Alfonso

et al. 2020

Int Forum Allergy Rhinol

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allergic rhinitis cat mucosal brush biopsy local IgE MBB How to Cite this Article Davison W Alfonso D An A Reisacher WR Nasal mucosal brush biopsy for cat allergy Int Forum Allergy Rhinol-W e present preliminary data estimating the sensitivity, specificity, and predictive value of nasal mucosal brush biopsy (MBB) for the measurement of specific immunoglobulin E (sIgE) against cat dander. Allergic rhinitis (AR) affects 10% to 30% of adults. 1 In 2016, 1.4% of U.S. physician visits resulted in the diagnosis. 2 The most common tests for AR, serum IgE detection and skin-prick testing (SPT), have significant falsepositive rates. In addition, sIgE can be detected in locally symptomatic regions of the aerodigestive tract despite negative SPT and in vitro testing. MBB has detected local sIgE in people with AR, nonallergic rhinitis, food allergy, and eosinophilic esophagitis. 3 Sensitivity and specificity estimates for MBB using respiratory allergens, however, has not been performed. Determining optimal testing cutoff levels is important for accurate environmental control counseling and immunotherapy decisions. Cat dander was chosen because of its high prevalence (12.1% in the United States) and self-identifiable nature. 4

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Section: Discussionsupporting

confidence: 46%

“…In a recent study, 95% of participants had nasal sIgE above a 0.10 kU/L cutoff, but only 40% had serum levels above this cutoff. 8 Similarly, Zhang et al 9 found that in children with adenotonsillar hypertrophy who tested negative for serum sIgE, local sIgE was detected in 44% of tonsils and 36% of adenoids.…”

Section: Discussionmentioning

confidence: 96%

Nasal mucosal brush biopsy for cat allergy

Davison

Alfonso

et al. 2020

Int Forum Allergy Rhinol

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“…However, they found more positive nasal spIgE among the control population (AR vs NAR vs healthy control: 73 vs 25 vs 43%, P = .04). Reisacher 27 also found high positivity among the healthy controls (95%) at the cutoff value of 0.1 kUA/L.…”

Section: Discussionmentioning

confidence: 85%

Optimizing Protein Harvest From Nasal Brushings for Determining Local Allergy Responses

Saricilar

Hamizan

Alvarado

et al. 2018

Am J Rhinol�Allergy

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Background Rhinitis is a highly prevalent yet often misdiagnosed condition. Patients who have local allergic rhinitis are regularly mislabeled as having a nonallergic etiology. Thus, a highly accurate, reproducible, and noninvasive assessment, which can be performed quickly and with minimal discomfort to the patient, is required. Objective The aim of this research was to identify the efficiency of various nasal brushes as tools for harvest and collection of epithelial proteins and its suitability for identification of rhinitis. Methods Nasal epithelial mucosa samples were taken from patients undergoing turbinate surgery using a cytology brush, a dental brush, and a nasal curette in random order. After washing in phosphate-buffered saline, the suspended cells were sonicated. Total protein content was assessed for all samples by bicinchoninic acid assay measured using a Nanodrop machine. Identification of nasal-specific immunoglobulin E (spIgE) was then assessed using immunoassay and compared to the patient's allergic status from epicutaneous and serum testing. The lower threshold limit for the spIgE in nasal brushings was determined using the results of serum spIgE tests as the reference. The diagnostic accuracy of this new established cutoff value was determined. Results The cytology brush was found to be the optimal tool for maximal nasal mucosa protein collection followed by dental brush and nasal curette (0.75 ± 0.45 mg/mL vs 0.43 ± 0.24 mg/mL vs 0.071 ± 0.55 mg/mL, respectively; P < .01). The optimal cutoff value of nasal spIgE from the cytology nasal brushings was 0.14 kUA/L to predict allergic status from serum testing. This gave a sensitivity of 75%, specificity of 86%, positive predictive value of 74%, likelihood ration positive of 5.40, and diagnostic odds ratio of 18.62. Conclusion The cytology brush is the optimal tool for protein collection. This is an easy and direct method to sample the nasal mucosa for assessment of nasal allergy or future biomarkers.

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“…Over 45% of those with AR may also have LAR. The detection of nasal sIgE can be accomplished by a variety of techniques, including nasal lavage, cellulose discs, mucosal biopsy, brushing, and others (29) .…”

Section: Indications For Serum Ige Testingmentioning

confidence: 99%

Assessment of Allergic Rhinitis from Medical Microbiology Background: Review Article

Rashad¹,

Bahgat²,

Ali³

et al. 2023

The Egyptian Journal of Hospital Medicine

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Background: Sneezing, postnasal drip, nasal pruritis, and nasal congestion are all signs of allergic rhinitis (AR), an atopic condition. It affects one in six people and is linked to high morbidity, severe productivity loss, and high healthcare expenses. In the past, AR was believed to be a nasal airway-specific disease. However, the emergence of the unified airway hypothesis has identified the atopic dermatitis (AD) and other related disorders, such as asthma, as components of systemic allergic reaction.Objective: Review of literature about assessment of Allergic Rhinitis from Medical Microbiology Background Methods: We searched PubMed, Google Scholar, and Science Direct for relevant articles on allergic rhinitis and medical microbiology background. However, only the most recent or thorough studies were taken into account between February 2001 and May 2023. The authors also evaluated the value of resources culled from other works in the same genre. Therefore, documents written in languages other than English have been ignored due to a lack of translation funds. Unpublished works, oral presentations, conference abstracts, and dissertations were generally agreed upon not to qualify as scientific research. Conclusion: Due to changes in the immune system, AR is an IgE-mediated illness that develops in genetically vulnerable people after exposure to environmental allergens. The majority of the common allergens linked to AR are proteins and glycoproteins present in airborne particles. As a result of the inhalation of allergen particles, the nasal epithelium becomes coated, allowing soluble allergenic proteins to elute and diffuse into the nasal mucosa. Numerous aeroallergens enhance allergen entry to antigen presentating cells (APCs) during the early sensitization process. In AR tight junctions in the airway epithelium are cleaved and epithelial cells are activated thanks to the protease activities of these proteins. Skin prick testing is used to confirm an AR diagnosis alongside a patient's history and a physical exam.

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Total and allergen‐specific immunoglobulin E in the serum and nasal mucosa of a nonallergic population

Cited by 8 publications

References 21 publications

Nasal mucosal brush biopsy for cat allergy

Nasal mucosal brush biopsy for cat allergy

Optimizing Protein Harvest From Nasal Brushings for Determining Local Allergy Responses

Assessment of Allergic Rhinitis from Medical Microbiology Background: Review Article

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