Total body water and ECFV measured using bioelectrical impedance analysis and indicator dilution in horses

Abstract

The purposes of this study were 1) to determine the compartmentation of body water in horses by using indicator dilution techniques and 2) to simultaneously measure bioelectrical impedance to current flow at impulse current frequencies of 5 and 200 kHz to formulate predictive equations that could be used to estimate total body water (TBW), extracellular fluid volume (ECFV), and intracellular fluid volume (ICFV). Eight horses and ponies weighing from 214 to 636 kg had catheters placed into the left and right jugular veins. Deuterium oxide, sodium thiocyanate, and Evans blue were infused for the measurement of TBW, ECFV, and plasma volume (PV), respectively. Bioelectrical impedance was measured by using a tetrapolar electrode configuration, with electrode pairs secured above the knee and hock. Measured TBW, ECFV, and PV were 0.677 +/- 0.022, 0.253 +/- 0.006, and 0.040 +/- 0.002 l/kg body mass, respectively. Strong linear correlations were determined among measured variables that allowed for the prediction of TBW, ECFV, ICFV, and PV from measures of horse length or height and impedance. It is concluded that bioelectrical impedance analysis (BIA) can be used to improve the predictive accuracy of noninvasive estimates of ECFV and PV in euhydrated horses at rest.

Time course and magnitude of changes in total body water, extracellular fluid volume, intracellular fluid volume and plasma volume during submaximal exercise and recovery in horses

“…Fluid volumes were measured using indicator dilution techniques as described previously 14 . Deuterium oxide (Sigma Chemical Co., St. Louis, MO), for measuring TBW, was infused as a 50% v/v solution with 0.9% sterile saline for a total volume of , 50 ml per horse.…”

Bioelectrical impedance analysis for the prediction of fat-free mass in buffalo calf

“…This technology, because of its accuracy, simplicity and portability, has been conveniently applied for pig (Swantek et al, 1991 and, sheep (Jenkins et al, 1988;Berg et al, 1996), beef cattle (Marchello and Slanger, 1992;Marchello et al, 1999;Velazco et al, 1999) and horse (Forro et al, 2000).…”

Estimation of Acute Fluid Shifts Using Bioelectrical Impedance Analysis in Horses

“…Weight change, biochemical indices, and dilutional techniques have been used to estimate fluid losses or gains, but these have limitations. [1][2][3][4][5][6][7][8] Specifically, weight changes cannot distinguish between losses from the extracellular fluid volume (ECFV) and intracellular fluid volume (ICFV). Knowing the balance between these specific fluid spaces is essential to guiding therapy in the clinical setting.…”

“…1,2,6,[9][10][11] It uses an alternating low-frequency electrical current to make predictions about body composition based on the assumption that fat and bone are poor conductors of electricity, as opposed to biological fluids that conduct currents well. MF-BIA is capable of providing rapid, noninvasive estimates of ECFV, ICFV, and TBW by determining the resistance and reactance properties of tissues.…”