Total Phenolic Content, Radical Scavenging Properties, and Essential Oil Composition ofOriganumSpecies from Different Populations

Abstract

The aim of this work was to compare the antiradical activity, total phenol content (TPC), and essential oil composition of Origanum vulgare spp. virens, Origanum x applii, Origanum x majoricum, and O. vulgare spp. vulgare cultivated in Argentina in different localities. The experiment was conducted in the research station of La Consulta (INTA-Mendoza), the research station of Santa Lucia (INTA-San Juan), and Agronomy Faculty of National University of La Pampa, from 2007 to 2008. The composition of the essential oils of oregano populations was independent of cultivation conditions. In total, 39 compounds were identified in essential oils of oregano from Argentina by means of GC-MS. Thymol and trans-sabinene hydrate were the most prominent compounds, followed by gamma-terpinene, terpinen-4-ol, and alpha-terpinene. O. vulgare vulgare is the only Origanum studied which is rich in gamma-terpinene. Among tested oregano, O. x majoricum showed the highest essential oil content, 3.9 mg g(-1) dry matter. The plant extract of O. x majoricum had greater total phenol content values, 19.36 mg/g dry weight, than the rest of oregano studied. To find relationships among TPC, free radical scavenging activity (FRSA), and climate variables, canonical correlations were calculated. The results obtained allow us to conclude that 70% of the TPC and FRSA variability can be explained by the climate variables (R(2) = 0.70; p = 8.3 x 10(-6)), the temperature being the most important climatic variable.

Estudio de la composición química del aceite esencial de orégano (Origanum vulgare spp.) de Tacna

“…Vulgare y Virens (OVV) han sido mencionadas por ser ricas en compuestos sesquiterpenoides (germacrene D), y monoterpenos (terpinen-4-ol) (Sezik, Tumen, Kirimer, Ozek y Baser, 1993;Kula, Majda, Stoyanova y Georgiev, 2007;Dambolena et al, 2010); sin embargo, los quimiotipos frecuentemente determinados son carvacrol y timol. Los factores principales y secundarios, como la herencia genética, edad de las plantas y elementos ambientales, influyen en sus concentraciones dentro de una misma especia (Poulose y Croteau, 1978).…”

Thermal stability and antioxidant activity of essential oils from aromatic plants farmed in Argentina

“…Phenol content was measured at 760 nm using a spectrophotometer (Hewlett Packard HP 8452 A, Palo Alto, CA, USA). Phenol content was expressed as mg/g (Dambolena et al, 2010).…”

Luteolin content and antioxidant activity in micropropagated plants of Poliomintha glabrescens (Gray)

“…Others compounds have presented different tendency (Ahmad et al 2010;Faizal et al 2011;Hayta et al 2011;Mohanty et al 2011;Shaik et al 2011). Dambolena et al (2010), who reported studies of several Argentinian oregano species, concluded that there was not evidence that total phenolics content is affected by environmental factors. Nonetheless, in this study we observed that in vitro conditions affected TP and ORAC increasing its contents and activities probably by factors related to in vitro conditions such as high humidity, water and light stress.…”