Total phenolic contents and antioxidant activity of Senna singueana, Melia azedarach, Moringa oleifera and Lannea discolor herbal plants

Mwamatope, Bonface; Tembo, David; Chikowe, Ibrahim; Kampira, Elizabeth; Nyirenda, Cecilia Maliwichi

doi:10.1016/j.sciaf.2020.e00481

Cited by 48 publications

(57 citation statements)

References 38 publications

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“…This assumption was also confirmed by correlation analysis, where we observed a linear correlation between total phenolics and the above-mentioned assays ( Figure 1 ). In addition, many authors reported a strong correlation between total phenolics and antioxidant properties [ 36 , 37 ]. As another antioxidant mechanism, chelation reflects the neutralization of transition metal and this could stop the production of the hydroxyl radical, which is the most dangerous radical.…”

Section: Resultsmentioning

confidence: 99%

Phytochemical Profile and Biological Activities of Crude and Purified Leonurus cardiaca Extracts

Angeloni

Maggi

Sagratini

et al. 2021

Plants

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Leonurus cardiaca L. (Lamiaceae) is a perennial herb distributed in Asia and Southeastern Europe and has been used in traditional medicine since antiquity for its role against cardiac and gynecological disorders. The polar extracts obtained from L. cardiaca aerial parts contain several compounds among which alkaloids, iridoids, labdane diterpenes, and phenylethanoid glycosides play a major role in conferring protection against the aforementioned diseases. On the other hand, the antioxidant activities and the enzyme inhibitory properties of these extracts have not yet been deeply studied. On the above, in the present study, crude and purified extracts were prepared from the aerial parts of L. cardiaca and have been chemically characterized by spectrophotometric assays and HPLC-DAD-MS analyses. Notably, the content of twelve secondary metabolites, namely phenolic acids (chlorogenic, caffeic, caffeoylmalic and trans-ferulic acids), flavonoids (rutin and quercetin), phenylethanoid glycosides (verbascoside and lavandulifolioside), guanidine pseudoalkaloids (leonurine), iridoids (harpagide), diterpenes (forskolin), and triterpenes (ursolic acid), has been determined. Furthermore, the extracts were tested for their antioxidant capabilities (phosphomolybdenum, DPPH, ABTS, FRAP, CUPRAC, and ferrous chelating assays) and enzyme inhibitory properties against cholinesterase, tyrosinase, amylase, and glucosidase. The purified extracts contained higher phytochemical content than the crude ones, with caffeoylmalic acid and verbascoside as the most abundant compounds. A linear correlation between total phenolics, radical scavenging activity, and reducing power of extracts has been found. Notably, quercetin, caffeic acid, lavandulifolioside, verbascoside, chlorogenic acid, rutin, and ursolic acid influenced the main variations in the bioactivities found in L. cardiaca extracts. Our findings provide further insights into the chemico-biological traits of L. cardiaca and a scientific basis for the development of nutraceuticals and food supplements.

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Section: Resultsmentioning

confidence: 99%

Phytochemical Profile and Biological Activities of Crude and Purified Leonurus cardiaca Extracts

Angeloni

Maggi

Sagratini

et al. 2021

Plants

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“…Extraction of phenolic compounds was achieved as previously described. [33][34][35][36] The powdered samples (25g) were soaked in 80% v/v methanol (300mL) for 72 hr while intermittently stirring and then filtered thereafter. Rotor evaporator (Buchi R-215, Switzerland) was used to concentrate the filtrates at 40°C and low pressure.…”

Section: Sample Extractionmentioning

confidence: 99%

“…[37] Semi-solid extracts (0.1g) were dissolved in hydrochloric acid (2M, 5.0mL), filtered and analysed. [36] Total phenolic content determination Total phenolic content was analysed using Folin-Ciocalteu assay. [36,38] The effectiveness of this method is on ability of phenolic compounds to transfer electrons in a basic environment to complexes of phosphomolybdic or phosphotungstic acid which, when reduced, produce a blue colour.…”

Section: Sample Extractionmentioning

confidence: 99%

“…[36] Total phenolic content determination Total phenolic content was analysed using Folin-Ciocalteu assay. [36,38] The effectiveness of this method is on ability of phenolic compounds to transfer electrons in a basic environment to complexes of phosphomolybdic or phosphotungstic acid which, when reduced, produce a blue colour. This colour is measured spectrophotometrically at 765 nm and absorption is proportional to concentration of phenolic compounds.…”

Section: Sample Extractionmentioning

confidence: 99%

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Seasonal Variation of Phytochemicals in Four Selected Medicinal Plants

Mwamatope¹,

Tembo²,

Kampira³

et al. 2021

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Background:The study aimed at assessing total alkaloids content (TAC), total flavonoids content (TFC), total phenolic content (TPC), antioxidant activity (AA) and predominant individual phenolic compounds in methanol extracts of M. oleifera, S.singueana, M.azedarach leaves and stem barks of L. discolor collected during different seasons of the year. Materials and Methods: TPC, TFC and TAC were analyzed using the Folin Ciocalteau assay, aluminum chloride assay and bromocresol green assay, respectively. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) techniques were used to analyse AA. Phenolic compounds; Vanillic acid, (+)-catechin, quercetin, rutin, sinapic acid, 2,4-hydroxybenzoic acid and ferulic acid were analyzed using high performance liquid chromatography diode array detector (HPLC -DAD). Results: Barks of L. discolour exhibited high levels of TPC, TFC, TAC and AA during hot-dry season (summer) as compared to S. singueana, M. oleifera and M. azedarach leaves which showed significantly (p< 0.05) higher levels in hot-wet season (rainy). Levels of TPC, TFC and TAC were significantly (p< 0.05) influenced by seasonal variations and correlated with levels of AA. Profiles of all phenolic compounds analyzed were not similar in terms of chemical composition and concentration during different seasons. Sinapic acid and 2,4-hydroxybenzoic acid were predominant in S. singueana while sinapic acid and ferulic acid were predominant in M. oleifera. Vanillic acid and 2,4-hydroxybenzoic acid were predominant in L. discolor and M. azedarach respectively. Conclusion: The phytochemical constituents in medicinal plants are subject to seasonal variations which in turn influence the optimum season of harvesting.

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“…It is reported that polyphenols and flavonoids are used for the prevention and cure of many diseases associated with free radicals and possess a broad spectrum of chemical and biological activity [11,20]. Flavonoids and phenols are considered as an important secondary metabolite containing at least one hydroxyl group that acts as freeradical scavengers and inhibitors of the hydrolytic and oxidative enzyme are known to have a profound anti-inflammatory action and health beneficial effects [8,11,25]. The presence of these metabolites in all the plant species though in different amounts is a significant finding of the present study.The phenolic compounds are also known to contribute toward quality and nutritional value through modifying color, taste, aroma, and flavor [26] whereas flavonoids are reported to be accountable for the chelating activity which helps in the reduction of oxidative stress [20].…”

Section: Determination Of Total Polyphenolic and Flavonoid Contentmentioning

confidence: 99%

Comparative Study of Polyphenol, Flavonoid, and Antioxidant Activity of Various Medicinal Plants Collected From Different Altitudes

Joshi

Bhandari

Shrestha

et al. 2021

Asian J Pharm Clin Res

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Objectives: The main aim of this investigation is to explore the folklore medicinal flora located at Terai and the lesser Himalayan region of Nepal and has a comparative study on phenol, flavonoid, and antioxidant activity that would bring forth a factual basis for its use in the traditional therapy of various health ailments. Methods: Folin-Ciocalteu and Colorimetric aluminum chloride methods were used for the estimation of total polyphenolic and flavonoid content, respectively. Moreover, antioxidant activity was determined by DPPH radical scavenging activity. Results: Among 21 plants collected, Artemesia vulgaris depicted higher (53±0.03 mg GAE/g) and Mimosa pudica (3.7±0.04 mg GAE/g) depicted lower phenolic content whereas the highest flavonoid content is observed in Syzygium cumini and the lowest value in Mentha piperita. Almost all the collected specimen demonstrated antioxidant activity, among which Eupatorium adenophorum and Rhododendron anthopogon demonstrated lower and higher antioxidant activity, respectively. Moreover, phenol and flavonoid content showed a weak correlation with the antioxidant activity indicating the major antioxidant to be different compounds other than phenols or flavonoids. Conclusion: The present study confirms the antioxidant activity of the collected plant specimen and defends its ethnobiological use as a possible natural antioxidant. Furthermore, the result of antioxidant properties encourages their application in medicinal health, functional food, and biopharmaceutics.

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Total phenolic contents and antioxidant activity of Senna singueana, Melia azedarach, Moringa oleifera and Lannea discolor herbal plants

Cited by 48 publications

References 38 publications

Phytochemical Profile and Biological Activities of Crude and Purified Leonurus cardiaca Extracts

Phytochemical Profile and Biological Activities of Crude and Purified Leonurus cardiaca Extracts

Seasonal Variation of Phytochemicals in Four Selected Medicinal Plants

Comparative Study of Polyphenol, Flavonoid, and Antioxidant Activity of Various Medicinal Plants Collected From Different Altitudes

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