Total reflection X-ray fluorescence mercury analysis after immobilization on quartz surfaces

Aretaki, I.N.; Koulouridakis, Pavlos; Kallithrakas‐Kontos, N.

doi:10.1016/j.aca.2006.01.084

Cited by 23 publications

(14 citation statements)

References 21 publications

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“…Interestingly, although CDI and GOPS surfaces provided similar densities of immobilized capture probe, CDI-activated surfaces allowed much greater hybridization densities than GOPSfunctionalized surfaces (0.072±0.02 versus 0.013± 0.003 pmol/cm 2 ). There are a few published reports describing directly linking proteins and ligands to inorganic substrates by CDI activation [12, 41]; however, to the best of our knowledge, this is the first published report immobilizing DNA directly to a silicon substrate via CDI activation. These results suggest that such a bioconjugation chemistry provides a viable alternative to the more traditional organofunctional silane.…”

Section: Resultsmentioning

confidence: 99%

“…In the case of many optical biosensors [9–11], it is important to limit the distance between the sensor surface and the immobilized probe in order to contain the hybridization event within the region of highest optical intensity. There are a few recent reports on conjugation of various ligands [12] and proteins [13] to silicon supports by 1,1-carbonyldiimidazole (CDI), indicating that it may be possible to directly link DNA capture probes to silicon substrates. Bypassing the need for a silane monolayer would bring the hybridization event closer to the substrate, a potential benefit to label-free detections.…”

Section: Introductionmentioning

confidence: 99%

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Bioconjugation techniques for microfluidic biosensors

Goddard

Erickson

2009

Anal Bioanal Chem

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We have evaluated five bioconjugation chemistries for immobilizing DNA onto silicon substrates for microfluidic biosensing applications. Conjugation by organosilanes is compared with linkage by carbonyldiimidazole (CDI) activation of silanol groups and utilization of dendrimers. Chemistries were compared in terms of immobilization and hybridization density, stability under microfluidic flow-induced shear stress, and stability after extended storage in aqueous solutions. Conjugation by dendrimer tether provided the greatest hybridization efficiency; however, conjugation by aminosilane treated with glutaraldehyde yielded the greatest immobilization and hybridization densities, as well as enhanced stability to both shear stress and extended storage in an aqueous environment. Direct linkage by CDI activation provided sufficient immobilization and hybridization density and represents a novel DNA bioconjugation strategy. Although these chemistries were evaluated for use in microfluidic biosensors, the results provide meaningful insight to a number of nanobiotechnology applications for which microfluidic devices require surface biofunctionalization, for example vascular prostheses and implanted devices.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bioconjugation techniques for microfluidic biosensors

Goddard

Erickson

2009

Anal Bioanal Chem

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“…Other analytical methods for mercury content determination are presented in many publications [8][9][10][11][12][13][14][15][16][17][18][19]. They are based on the following detection methods:…”

Section: Comparison With Other Methodsmentioning

confidence: 99%

The method of determination of mercury adsorption from flue gases

et al. 2017

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Abstract. For several recent years Faculty of Energy and Fuels of the AGH University of Science and Technology in Krakow conduct intensive studies on the occurrence of mercury contained in thermal and coking coals, as well as on the possible reduction of fossil-fuel mercury emissions. This research focuses, among others, on application of sorbents for removal of mercury from flue gases. In this paper we present the methodology for testing mercury adsorption using various types of sorbents, in laboratory conditions. Our model assumes burning a coal sample, with a specific mercury content, in a strictly determined time period and temperature conditions, oxygen or air flow rates, and the flow of flue gases through sorbent in a specific temperature. It was developed for particular projects concerning the possibilities of applying different sorbents to remove mercury from flue gases. Test stand itself is composed of a vertical pipe furnace inside which a quartz tube was mounted for sample burning purposes. At the furnace outlet, there is a heated glass vessel with a sorbent sample through which flue gases are passing. Furnace allows burning at a defined temperature. The exhaust gas flow path is heated to prevent condensation of the mercury vapor prior to contact with a sorbent. The sorbent container is positioned in the heating element, with controlled and stabilized temperature, which allows for testing mercury sorption in various temperatures. Determination of mercury content is determined before (coal and sorbent), as well as after the process (sorbent and ash). The mercury balance is calculated based on the Hg content determination results. This testing method allows to study sorbent efficiency, depending on sorption temperature, sorbent grain size, and flue-gas rates.

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“…Specifically, solid-state polymer-based membranes have been found versatile, selective, and easy to apply, since detection limits lower than ug•L −1 can be achieved with an energy dispersive X-ray fluorescence (EDXRF) (Hatzistavros and Kallithrakas-Kontos, 2014), a total reflection X-ray fluorescence (TXRF) technique (Aretaki et al, 2006;Koulouridakis and Kallithrakas-Kontos, 2004) or even with a benchtop TXRF system (Marguí et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Solid-state polymer membranes for simple, sensitive, and low-cost monitoring of mercury in water

Foteinis

Vazgiouraki

Karydas

et al. 2019

Science of The Total Environment

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Solid-state Hg(II) selective membranes were produced and assessed by means of X-ray absorption near edge structure in the total reflection X-ray fluorescence (XANES-TXRF) setup and by the energy dispersive X-ray fluorescence (EDXRF) technique. Membranes were functionalized using four promising ligands for mercury complexation, i.e.: i) 4-(2-Pyridylazo) resorcinol (PAR), ii) thiourea, iii) calconcarboxylic acid (CCS), and iv) dithizone. A simple analytical procedure was followed, using miniscule reagent quantities, thus suggesting the process is also cost-effective. XANES-TXRF revealed that mercury complexes with the ligands, and not simply adsorbed onto the PVC matrix, while the complexation was not affected by the matrix existence. Mercury exhibited an increased oxidation grade and was covalently bound to it was preferably bound with Cu in tap water, indicating limited usefulness for mercury preconcentration. Therefore, it is suggested that, depending on the ligand, the solid-state membranes could also possibly used for multi-elemental heavy metals analysis in water.

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Total reflection X-ray fluorescence mercury analysis after immobilization on quartz surfaces

Cited by 23 publications

References 21 publications

Bioconjugation techniques for microfluidic biosensors

Bioconjugation techniques for microfluidic biosensors

The method of determination of mercury adsorption from flue gases

Solid-state polymer membranes for simple, sensitive, and low-cost monitoring of mercury in water

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