Toward Integrated Molecular Diagnostic System (&lt;formula formulatype="inline"&gt;&lt;tex Notation="TeX"&gt;$i$&lt;/tex&gt;&lt;/formula&gt; MDx): Principles and Applications

Park, Seung Min; Sabour, Andrew F.; Son, Jun Ho; Lee, Sang Hun; Lee, Luke P.

doi:10.1109/tbme.2014.2309119

Cited by 18 publications

(8 citation statements)

References 132 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nanobiotechnology is now used routinely for in vitro diagnostics in the clinic 7 . For example, gold nano particles (GNPs) are used for pregnancy tests through a colorimetric assay of human chorionic gonadotropin (hCG) in urine by hCG antibody-conjugated GNPs.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Towards clinically translatable in vivo nanodiagnostics

et al. 2017

View full text Add to dashboard Cite

Nanodiagnostics as a field makes use of fundamental advances in nanobiotechnology to diagnose, characterize and manage disease at the molecular scale. As these strategies move closer to routine clinical use, a proper understanding of different imaging modalities, relevant biological systems and physical properties governing nanoscale interactions is necessary to rationally engineer next-generation bionanomaterials. In this Review, we analyse the background physics of several clinically relevant imaging modalities and their associated sensitivity and specificity, provide an overview of the materials currently used for in vivo nanodiagnostics, and assess the progress made towards clinical translation. This work provides a framework for understanding both the impressive progress made thus far in the nanodiagnostics field as well as presenting challenges that must be overcome to obtain widespread clinical adoption.

show abstract

mentioning

confidence: 99%

“…Because in vitro strategies are extensively covered elsewhere 7,9,10 , we focus on nanotechnologies that enhance diagnostic capabilities in vivo . We cover the background physics of several widely accepted imaging modalities and the materials used for in vivo nanodiagnostics, and provide examples of clinical translation.…”

mentioning

confidence: 99%

Towards clinically translatable in vivo nanodiagnostics

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Much of the effort has therefore been put into developing microfluidic-based systems for sample preparation upstream of bacterial detection. 184,188–197 For instance, Hung and Han’s team reported an inertial microfluidics to rapidly isolate bacteria from whole blood in a label-free fashion with the efficient recovery of even low-abundance bacteria (10–50 bacteria/mL) (Fig. 7).…”

Section: Microtechnologies For Sample Processing System Integrationmentioning

confidence: 99%

Emerging Microtechnologies and Automated Systems for Rapid Bacterial Identification and Antibiotic Susceptibility Testing

Xing²,

Zhao

2017

SLAS Technology

View full text Add to dashboard Cite

Rapid bacterial identification (ID) and antibiotic susceptibility testing (AST) are in great demand due to the rise of drug-resistant bacteria. Conventional culture-based AST methods suffer from a long turnaround time. By necessity, physicians often have to treat patients empirically with antibiotics, which has led to an inappropriate use of antibiotics, an elevated mortality rate and healthcare costs, and antibiotic resistance. Recent advances in miniaturization and automation provide promising solutions for rapid bacterial ID/AST profiling, which will potentially make a significant impact in the clinical management of infectious diseases and antibiotic stewardship in the coming years. In this review, we summarize and analyze representative emerging micro- and nanotechnologies, as well as automated systems for bacterial ID/AST, including both phenotypic (e.g., microfluidic-based bacterial culture, and digital imaging of single cells) and molecular (e.g., multiplex PCR, hybridization probes, nanoparticles, synthetic biology tools, mass spectrometry, and sequencing technologies) methods. We also discuss representative point-of-care (POC) systems that integrate sample processing, fluid handling, and detection for rapid bacterial ID/AST. Finally, we highlight major remaining challenges and discuss potential future endeavors toward improving clinical outcomes with rapid bacterial ID/AST technologies.

show abstract

“…21 To achieve this goal, different microfluidic functions must be implemented on a single chip. The actuation transmitter proposed in this study can be easily implemented on a single chip because the actuation transmitter is built with a single-layer design.…”

Section: An Application For Evaluating Rbc Extensibilitymentioning

confidence: 99%

On-chip actuation transmitter for enhancing the dynamic response of cell manipulation using a macro-scale pump

et al. 2015

View full text Add to dashboard Cite

An on-chip actuation transmitter for achieving fast and accurate cell manipulation is proposed. Instead of manipulating cell position by a directly connected macro-scale pump, polydimethylsiloxane deformation is used as a medium to transmit the actuation generated from the pump to control the cell position. This actuation transmitter has three main advantages. First, the dynamic response of cell manipulation is faster than the conventional method with direct flow control based on both the theoretical modeling and experimental results. The cell can be manipulated in a simple harmonic motion up to 130 Hz by the proposed actuation transmitter as opposed to 90 Hz by direct flow control. Second, there is no need to fill the syringe pump with the sample solution because the actuation transmitter physically separates the fluids between the pump and the cell flow, and consequently, only a very small quantity of the sample is required (<1 ll). In addition, such fluid separation makes it easy to keep the experiment platform sterilized because there is no direct fluid exchange between the sample and fluid inside the pump. Third, the fabrication process is simple because of the single-layer design, making it convenient to implement the actuation transmitter in different microfluidic applications. The proposed actuation transmitter is implemented in a lab-on-a-chip system for red blood cell (RBC) evaluation, where the extensibility of red blood cells is evaluated by manipulating the cells through a constriction channel at a constant velocity. The application shows a successful example of implementing the proposed transmitter. V C 2015 AIP Publishing LLC.

show abstract

Toward Integrated Molecular Diagnostic System (<formula formulatype="inline"><tex Notation="TeX">$i$</tex></formula> MDx): Principles and Applications

Cited by 18 publications

References 132 publications

Towards clinically translatable in vivo nanodiagnostics

Towards clinically translatable in vivo nanodiagnostics

Emerging Microtechnologies and Automated Systems for Rapid Bacterial Identification and Antibiotic Susceptibility Testing

On-chip actuation transmitter for enhancing the dynamic response of cell manipulation using a macro-scale pump

Contact Info

Product

Resources

About