Toward reducing biomaterial antigenic potential: a miniaturized Fc-binding domain for local deposition of antibodies

Pham, Ngoc Bich; Liu, Wen; Schueller, Nathan R.; Gawalt, Ellen S.; Fan, Yong; Meng, Wilson S.

doi:10.1039/c8bm01220b

Cited by 9 publications

(14 citation statements)

References 41 publications

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“…Designer self‐assembling peptide hydrogels span over past three decades from serendipitous discovery of the first self‐assembling peptide EAK16‐II in 1990 to a large range of biomedical applications, including cell or drug and antibody carriers, [ 66 ] stem cell scaffolds, [ 9a,67 ] microtissue formation in vitro, [ 65 ] and novel peptide detergents or surfactants. [ 23c ] The other self‐assembling peptide elements have emerged by a similar molecular design strategy in the past decade years ( Figure ), mainly including glutamine‐rich peptides, β‐hairpin peptides, α‐helix peptides, coiled‐coil peptides, multidomain peptide, and aromatic short peptide derivatives (partially indicated in Table 2 ).…”

Section: Common Hydrogel Products and Biomedical Featuresmentioning

confidence: 99%

“…In designer self‐assembling peptide hydrogels, the canonical amino acid residues serve as basic molecular building blocks of nanofiber scaffold networks, which confer inherent biocompatibility, high hydrophilicity, chemical amenability, and biodegradation in vivo. Because of these prominent advantages, designer self‐assembling peptide hydrogels are proposed to fabricate various 3D ex vivo microtissue models, [ 65 ] tumor organoids for preclinical drug discovery, [ 80 ] 3D cell culture constructs for clinical drug repositioning, [ 3 ] therapeutic drug or cell delivery carrier, [ 66b,81 ] and new generation self‐adjuvant vaccine design. [ 82 ] Over the past decades, extensive biomedical researches and translational and clinical trials greatly enlarged our understanding of basic cancer research.…”

Section: Common Hydrogel Products and Biomedical Featuresmentioning

confidence: 99%

“…A) Human stem cell‐based cell construct provides alternative options for peripheral nerve regeneration and repair strategies in RADA16‐I hydrogel, [ 65 ] in addition to the cell construct in EFK‐I hydrogel, which is effectively injectable cell delivery platform for intervertebral disc repair applications. [ 66b ] B) P11 peptide hydrogel directly indicates in situ clinical treatment benefit for early buccal carious lesions. [ 216 ] Other designer peptide hydrogels are the effective cell scaffolds for periodontal regeneration or dental pulp‐derived stem cell transplantation.…”

Section: Instructive Cell Constructs In Tissue Engineering and Precismentioning

confidence: 99%

“…[ 59,60,62 ] As the biomimetic cell constructs are approved in medicinal products, the advantages and benefits of designer self‐assembling peptide hydrogels are clear for the translation to a clinical platform setting, which open the ways to high‐quality clinical trials to explore the usefulness of cell construct‐based therapy by biomedical nanotechnology. [ 49,66b ] So, the main efforts can be put into the researches involved in complex human diseases, such as prostate cancer, [ 114b ] breast cancer. [ 143 ] Herein, we focus on the several main scientific aspects involved in precise oncology remodeling of ovarian cancer and delineate the potential frontiers for future researches.…”

Section: Instructive Cell Constructs In Tissue Engineering and Precismentioning

confidence: 99%

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Designer Self‐Assembling Peptide Hydrogels to Engineer 3D Cell Microenvironments for Cell Constructs Formation and Precise Oncology Remodeling in Ovarian Cancer

Yang

Zhao

2020

Advanced Science

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mouse intestinal stem cells and primary colorectal cancer cells can be propagated in vitro long term, [1] there is an urgent need to develop more physiologically relevant, efficient, and robust precise oncology models that closely recapitulate the genetic and morphological heterogeneous composition and mimic the arrangement pattern of cancer cells in the original tumor. [2] To our knowledge in biomedical research, hydrogel is the best tool to reconstruct precise oncology models in vitro, especially tumor organoid, which not only recapitulates in vivo biology and microenvironmental factors, but also at large extent allows side-by-side comparison to evaluate the translational potential of 3D model systems to the patients. [2a,3] Generally, hydrogels are mainly composed of hydrophilic polymeric scaffolds that absorb large amounts of water, so the hydrogel matrix better mimics elastic and viscoelastic properties in ECM and microscale topographies of cell matrices, which controls proper cell morphology, directs viable cell behaviors, and drives in vivo fundamental cell-cell or cell-ECM interactions. [4] To recapitulate pathophysiological features of human tumors and imitate various aspects of human tumorigenesis in vivo, hydrogels can provide a realistic platform to establish a useful bridge between in vitro assays and in vivo cell microenvironments. In current biomedical applications, there are many kinds of hydrogels to be developed to mimic the biological properties of ECM, such Designer self-assembling peptides form the entangled nanofiber networks in hydrogels by ionic-complementary self-assembly. This type of hydrogel has realistic biological and physiochemical properties to serve as biomimetic extracellular matrix (ECM) for biomedical applications. The advantages and benefits are distinct from natural hydrogels and other synthetic or semisynthetic hydrogels. Designer peptides provide diverse alternatives of main building blocks to form various functional nanostructures. The entangled nanofiber networks permit essential compositional complexity and heterogeneity of engineering cell microenvironments in comparison with other hydrogels, which may reconstruct the tumor microenvironments (TMEs) in 3D cell cultures and tissue-specific modeling in vitro. Either ovarian cancer progression or recurrence and relapse are involved in the multifaceted TMEs in addition to mesothelial cells, fibroblasts, endothelial cells, pericytes, immune cells, adipocytes, and the ECM. Based on the progress in common hydrogel products, this work focuses on the diverse designer self-assembling peptide hydrogels for instructive cell constructs in tissue-specific modeling and the precise oncology remodeling for ovarian cancer, which are issued by several research aspects in a 3D context. The advantages and significance of designer peptide hydrogels are discussed, and some common approaches and coming challenges are also addressed in current complex tumor diseases.

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Section: Common Hydrogel Products and Biomedical Featuresmentioning

confidence: 99%

Section: Common Hydrogel Products and Biomedical Featuresmentioning

confidence: 99%

Section: Instructive Cell Constructs In Tissue Engineering and Precismentioning

confidence: 99%

Section: Instructive Cell Constructs In Tissue Engineering and Precismentioning

confidence: 99%

See 2 more Smart Citations

Designer Self‐Assembling Peptide Hydrogels to Engineer 3D Cell Microenvironments for Cell Constructs Formation and Precise Oncology Remodeling in Ovarian Cancer

Yang

Zhao

2020

Advanced Science

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“…Fc binding domain from protein G from Streptococcus 3B [82] EAK16-II ([AEAEAKAK] 2 ) FNMQAQRRFYEALHD 3B [83] CysPA (CGGKKKAAVVK[C 11 ]-NH 2 ) Cys/RGDS/gold nanoparticles 2 [84] Yellow fluorescent protein/cyan fluorescent protein 2 [84] FHV nanoparticles Maltose-binding protein 2 [85] (Continues)…”

Section: Assembly Motifmentioning

confidence: 99%

Multivalent display of chemical signals on self‐assembled peptide scaffolds

et al. 2021

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Self-assembled peptide materials have emerged as promising bioinspired tools for applications that include regenerative medicine, drug delivery, antimicrobial and vaccine development, optics, and catalysis. Peptide self-assembly mediated by noncovalent hydrogen bonding, coulombic, hydrophobic, and aromatic interactions gives rise to a variety of supramolecular structures that reflect on the nature of the constituent peptides. The emergent properties of these supramolecular peptide materials often depend on the multivalent presentation of functional appendages on the self-assembled scaffold. For example, the multivalent display of cell-signaling motifs on self-assembled peptide nanofibrils provides materials that are excellent extracellular matrix mimetics for tissue engineering applications. This review includes a discussion of chemical strategies that address the challenge of appending functional signal motifs in a multivalent display on self-assembled peptide and protein materials. In addition, recent examples of supramolecular peptide materials that rely on the multivalent display of chemical signals for the desired applications are presented. Collectively, this discussion illustrates the potential of self-assembled peptides as sustainable materials to address challenges in contemporary materials science and provides principles for the design of next-generation agents for a variety of applications.

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Chemically‐Induced Cross‐Linking of Peptidic Fibrils for Scaffolding Polymeric Particles and Macrophages

Armen

Schueller

Velankar

et al. 2021

Macromolecular Bioscience

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EAK16‐II (EAK) is a self‐assembling peptide (SAP) that forms β‐sheets and β‐fibrils through ionic‐complementary interactions at physiological ionic strengths. The soft materials can be injected in vivo, creating depots of drugs and cells for rendering pharmacological and biological actions. The scope of the applications of EAK is sought to extend to tissues through which the flow of extracellular fluid tends to be limited. In such anatomical locales the rate and extent of the fibrilization are limited insofar as drug delivery and cellular scaffolding would be impeded. A method is generated utilizing a carbodiimide cross‐linker by which EAK fibrils are pre‐assembled yet remain injectable soft materials. It is hypothesized that the resulting de novo covalent linkages enhance the stacking of the β‐sheet bilayers, thereby increasing the lengths of the fibrils and the extent of their cross‐linking, as evidenced in Diffuse Reflectance Infrared Fourier Transform (DRIFT) spectroscopy, scanning electron microscopy, and atomic force microscopy analyses. The cross‐linked EAK (clEAK) retains polymeric microspheres with an average diameter of 1 µm. Macrophages admixed with clEAK remain viable and do not produce the inflammatory mediator interleukin‐1β. These results indicate that clEAK should be investigated further as a platform for delivering particles and cells in vivo.

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Toward reducing biomaterial antigenic potential: a miniaturized Fc-binding domain for local deposition of antibodies

Abstract: A novel bi-functional peptide consisting of an Fc-binding domain and self-assembly sequence is shown to retain IgG in vivo.

Cited by 9 publications

References 41 publications

Designer Self‐Assembling Peptide Hydrogels to Engineer 3D Cell Microenvironments for Cell Constructs Formation and Precise Oncology Remodeling in Ovarian Cancer

Designer Self‐Assembling Peptide Hydrogels to Engineer 3D Cell Microenvironments for Cell Constructs Formation and Precise Oncology Remodeling in Ovarian Cancer

Multivalent display of chemical signals on self‐assembled peptide scaffolds

Chemically‐Induced Cross‐Linking of Peptidic Fibrils for Scaffolding Polymeric Particles and Macrophages

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