5G Stand Alone (SA) networks are starting to be considered, designed and implemented in multiple countries in various forms (public, private, experimental). 5G SA networks mass adoption is expected to materialize by 2025. Mass deployment is anticipated at a large scale, due to the rich features and capabilities offered by 5G networks, including but not limited to slicing, service orchestration and automation, bringing the benefits of 5G among industry stakeholders and verticals. The concept of Network Applications is gaining momentum, as a way to ease the process of deploying industry-specific services and applications and to integrate them seamlessly with the new 5G networks and customer-specific application components. We target deploying and operating the novel 5G SA testbeds, Network Application and related capabilities in different T&L facilities across Europe. We envision the architectural advancement in terms of 5G features, such as orchestration, multi-slice implementation, Quality of Service (QoS)/Quality of Experience (QoE) and an innovative end-to-end monitoring framework, for network and application KPIs. In this paper, 5G open testbed advancements (3GPP Rel. 16 compliant) and readiness for Network Application experiments in real-life scenarios are presented, integrated as a unitary whole within the EU-funded VITAL-5G project.