Towards a molecular identification and classification system of lepidopteran-specific baculoviruses

Lange, Martin; Wang, Hualin; Hu, Zhihong; Jehle, Johannes A.

doi:10.1016/j.virol.2004.04.023

Cited by 106 publications

(100 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The Lepidopteran NPVs were separated into two groups, group I and II, based on the conserved gene, polyhedron, which encoded the major occlusion body protein required for producing viral progeny 3,5 . Later, the phylogenetics of NPVs were generated with other conserved genes, www.scienceasia.org for example lef-2 33 , lef-8, lef-9 34,35 , and ecdysteroid UBP-glycosyl transferase (egt) 4 . Recently, many phylogenetics of NPVs have been established by means of the combination of several conserved gene sequences and whole genome sequences 7,36,37 .…”

Section: Phylogenetic Analysis Of Group Imentioning

confidence: 99%

“…These phylogenetic trees were suggested to be more acceptable than those constructed by a single gene. However, the tree constructed by a single gene had been used for quick identification 34 . In this work, even though ie-1 gene was poorly conserved, the pattern of the tree topology derived from IE-1 amino acid sequences agreed with those of other tree topologies based on single highly conserved genes, the combination of highly conserved genes, and complete genome sequences.…”

Section: Phylogenetic Analysis Of Group Imentioning

confidence: 99%

See 1 more Smart Citation

Untitled

Chotimanothum¹,

Pinpart²,

Ngernsiri³

2011

ScienceAsia

View full text Add to dashboard Cite

The immediate early 1 (ie-1) gene is essential for DNA replication of nucleopolyhedrosis virus. Here, the gene ie-1 from nucleopolyhedrovirus (Nanglai-BmNPV) infecting a local silkworm (Bombyx mori), Nanglai strain, was cloned and sequenced. The gene contained a 1755 bp open reading frame encoding a deduced protein of 584 amino acids, molecular weight 66.9 kDa, and isoelectric point 5.79. The nucleotide and amino acid sequences of Nanglai-BmNPV ie-1 gene showed, respectively, 98 and 97% identity to those of the BmNPV ie-1 genes. The identity of the Nanglai-BmNPV IE-1 deduced amino acid sequence with IE-1 protein sequences of other 37 known Lepidopteran NPVs varied from 20% up to 97%. The phylogenic tree established with the IE-1 protein sequences of the Lepidopteran NPVs showed similar topology to those previously reported using highly conserved genes. Interestingly, the tree obtained with IE-1 clearly shows that the Lepidopteran NPVs group II clade can be subdivided into three subgroups.

show abstract

Section: Phylogenetic Analysis Of Group Imentioning

confidence: 99%

Section: Phylogenetic Analysis Of Group Imentioning

confidence: 99%

Untitled

Chotimanothum¹,

Pinpart²,

Ngernsiri³

2011

ScienceAsia

View full text Add to dashboard Cite

show abstract

“…In fact, a low level of idendity can be expected between SpliNPV-TR1 and NPVs isolated from different host families except Noctuidae and this would have not constituted a surprising situation. Lange et al (2004) reported that group II NPVs presently subsume all lepidopteran-specific NPVs not belonging to the monophyletic group I NPVs and the distribution of the virus host families so far suggests a strong coevolution between baculoviruses and insect host families. Herniou et al (2001) also reported that monophyly of the group II NPVs is far less well supported that that for the group I NPVs or the GVs.…”

Section: Splinpv-tr1mentioning

confidence: 99%

“…The virions are occluded in a polyhedral shaped occlusion body called polyhedra in the NPV genus and are protected against enviromental conditions for years (Toprak et al 2005a). About 600 baculoviruses are described to infect insects that belong mostly to the insect orders Lepidoptera, Hymenoptera, and Diptera (Martignoni and Iwai 1981) and about 90 % were reported from 34 different families of Lepidoptera (Lange et al 2004). …”

Section: Introductionmentioning

confidence: 99%

The Partial Sequence Comparison of lef-8 gene Obtained from a Turkish SpliNPV–B with Several Baculoviruses

;SUSURLUK¹

2005

Tarım Bilimleri Dergisi

View full text Add to dashboard Cite

Spodoptera littoralis Boisd. (Lepidoptera:Noctuidae) nucleopolyhedrovirus (SpliNPV) is a member of Baculoviridae and belongs to the group II NPVs. Lef-8 is a well-conserved gene that is characterized in most of the lepidopteran NPVs. Nucleotide and aminoacid sequences of the encoded proteins and gene content may be phylogenetically informative. In this study, lef-8 gene in the Turkish SpliNPV-B (SpliNPV-TR1) genome was partially amplified by PCR and analyzed by amino-acid sequences with several baculoviruses. Amino-acid sequences of partial SpliNPV-TR1 lef-8 gene was 84 % identical to the lef-8 of SpltNPV while revealed it to have about only 57 %, 55 %, 51 %, 50 %, 50 %, 50 % and 49 % of its amino-acid sequence identity with AgipNPV, McNPV, RoNPV, HaNPV-G4, AcNPV, HzNPV and BufuNPV, respectively. The sequences between SpliNPV-TR1 and granuloviruses also produced low levels of sequence identities (maximum 46 %). Although group II NPVs were rather isolated from noctuid hosts, the low level identity values detected between these noctuid NPVs (except the high idendity value between SpliNPV-TR1 and SpltNPV) revealed that these isolates can not have a monophyletic origin.Key Words: Baculovirus, spodoptera littoralis, noctuidae, spliNPV-TR1, lef-8, sequence, comparison. Türk SpliNPV-B İzolatından elde edilen lef-8 Geninin diğer Bakulovirüslerle Kısmi Sekans KarşılaştırmasıÖz: Spodoptera littoralis Boisd. (Lepidoptera:Noctuidae) nukleopolihedrovirus (SpliNPV) Baculoviridae'nin bir üyesi olup grup II NPV'leri arasında yer almaktadır. Lef-8 iyi korunmuş bir gen olup pek çok lepidopter NPV'sinde tanımlanmıştır. Kodlanan proteinlerin nükleotid ve aminoasit dizinleri ve gen içerikleri filogenetik olarak aydınlatıcı olabilmektedir. Bu çalışmada, Türk SpliNPV-B (SpliNPV-TR1) genomundaki lef-8 geni PCR'la kısmi olarak çoğaltılmış ve aminoasit dizinlerine göre bazı bakulovirüslerle analiz edilmiştir. SpliNPV-TR1 kısmi lef-8 geninin aminoasit dizinleri SpltNPV ile % 84 benzelik gösterirken AgipNPV, McNPV, RoNPV, HaNPV-G4, AcNPV, HzNPV ve BufuNPV ile sırasıyla sadece % 57, % 55 , % 51, % 50, % 50, % 50 ve % 49 aminoasit benzerliği göstermiştir. SpliNPV-TR1 ve granulovirüsler arasındaki sekanslar da düşük dizin benzerlikleri üretmiştir (en fazla % 46). Grup II NPV'leri esas olarak noctuid konukçulardan izole edilse de, bu noctuid NPV'leri arasında saptanan düşük dereceli benzerlikler (SpliNPV-TR1 ve SpltNPV arasındaki yüksek benzerlik oranı hariç), bu izolatların monofiletik bir orijine sahip olamayacağını göstermektedir.

show abstract

“…The BmNPV polyhedrin gene is non-essential late gene for BmNPV replication and the ORF contains 738 nucleotides 6 . The polh gene has not only been used in phylogenetic studies but also in the molecular identification of baculoviruses specific to lepidoptera [7][8][9][10] . Baculovirus-repeated ORFs (bro) are widespread among invertebrate viruses.…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification of a new Bombyx mori nucleopolyhedrovirus strain isolated from Yunnan, China

Fen-fen¹,

Zhang²,

Shao³

et al. 2017

ScienceAsia

View full text Add to dashboard Cite

ABSTRACT:Bombyx mori nucleopolyhedrovirus (BmNPV) is a silkworm pathogen that causes serious economic losses in sericulture. In this study, a new BmNPV strain, designated as BmNPV-YN1, from Yunnan (China), was isolated and identified through molecular identification. Sequence analysis results revealed that polyhedrin (polh) presented the highest identity among various BmNPVs; this gene indicated that BmNPV-YN1 is a BmNPV strain. The baculovirus repeated-ORF (bro) genes of this isolate were different from those of six BmNPV strains (BmNPV-T3, BmNPV-ZJ, BmNPV-GX, BmNPV-India, BmNPV-Cubic, and BmNPV-Brazilian). The BmNPV-YN1 bro family includes bro-a, bro-b, broc, bro-d, and bro-e, which are similar to those of BmNPV-T3. Sequence alignment results further showed the differences in the composition and sequences of the bro family members among various strains. The bro-d sequences were also phylogenetically analysed. Results demonstrate that BmNPV-YN1, BmNPV-Cubic, and BmNPV-India are closely related. Despite this close relationship, BmNPV-YN1 belongs to a distinct cluster. The BmNPV DNA was subjected to restriction endonuclease analysis and the obtained results provided additional evidence supporting the identity of the novel BmNPV-YN1 strain. Indeed, BmNPV-YN1 isolated from Yunnan is a new BmNPV strain. The proposed method can be used to rapidly and accurately detect and identify BmNPVs strains.

show abstract

Towards a molecular identification and classification system of lepidopteran-specific baculoviruses

Cited by 106 publications

References 34 publications

Untitled

Untitled

The Partial Sequence Comparison of lef-8 gene Obtained from a Turkish SpliNPV–B with Several Baculoviruses

Isolation and identification of a new Bombyx mori nucleopolyhedrovirus strain isolated from Yunnan, China

Contact Info

Product

Resources

About