Towards a personalized approach to aromatase inhibitor therapy: a digital microfluidic platform for rapid analysis of estradiol in core-needle-biopsies

Abdulwahab, Sara; Ng, Alphonsus H. C.; Chamberlain, M. Dean; Ahmado, Hend; Behan, Lucy Ann; Gomaa, Hala; Casper, Robert F.; Wheeler, Aaron R.

doi:10.1039/c7lc00170c

Cited by 28 publications

(16 citation statements)

References 25 publications

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“…The preparation of the EWOD system was based a custom method which uses Chitosan tagged magnetic beads to capture the target DNA. Magnetic beads have been used successfully in lab-based DNA extraction protocols [ 15 , 16 ]. To prepare the chip for the magnetic bead protocol, the chip is filled with 50 µL of 1cSt silicone oil.…”

Section: Methodsmentioning

confidence: 99%

Design of a Hand-Held and Battery-Operated Digital Microfluidic Device Using EWOD for Lab-on-a-Chip Applications

et al. 2021

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Microfluidics offer many advantages to Point of Care (POC) devices through lower reagent use and smaller size. Additionally, POC devices offer the unique potential to conduct tests outside of the laboratory. In particular, Electro-wetting on Dielectric (EWOD) microfluidics has been shown to be an effective way to move and mix liquids enabling many PoC devices. However, much of the research surrounding these microfluidic systems are focused on a single aspect of the system capability, such as droplet control or a specific new application at the device level using the EWOD technology. Often in these experiments the supporting systems required for operation are bench top equipment such as function generators, power supplies, and personal computers. Although various aspects of how an EWOD device is capable of moving and mixing droplets have been demonstrated at various levels, a complete self-contained and portable lab-on-a-chip system based on the EWOD technology has not been well demonstrated. For instance, EWOD systems tend to use high voltage alternating current (AC) signals to actuate electrodes, but little consideration is given to circuitry size or power consumption of such components to make the entire system portable. This paper demonstrates the feasibility of integrating all supporting hardware and software to correctly operate an EWOD device in a completely self-contained and battery-powered handheld unit. We present results that demonstrate a complete sample preparation flow for deoxyribonucleic acid (DNA) extraction and isolation. The device was designed to be a field deployable, hand-held platform capable of performing many other sample preparation tasks automatically. Liquids are transported using EWOD and controlled via a programmable microprocessor. The programmable nature of the device allows it to be configured for a variety of tests for different applications. Many considerations were given towards power consumption, size, and system complexity which make it ideal for use in a mobile environment. The results presented in this paper show a promising step forward to the portable capability of microfluidic devices based on the EWOD technology.

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Section: Methodsmentioning

confidence: 99%

Design of a Hand-Held and Battery-Operated Digital Microfluidic Device Using EWOD for Lab-on-a-Chip Applications

et al. 2021

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“…Indirect off-line methods are fairly straightforward and mainly focus on sample preparation prior to MS. An example is seen in Abdulwahab et al, 88 where the authors used a DMF system for on-chip tissue extraction and magnetic particle-based immunoassay to detect estradiol from mammalian tissue biopsies using high-performance liquid chromatography (HPLC)-MS/MS. The method can be executed from extraction to analysis within 40 min.…”

Section: Sample Preparation For Msmentioning

confidence: 99%

Role of Digital Microfluidics in Enabling Access to Laboratory Automation and Making Biology Programmable

Kothamachu¹,

Zaini²,

Muffatto³

2020

SLAS Technology

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“…Parafilm or double-sided tape. However, this requires more handling experience [39,[69][70][71][72][73][74][75][76][77][78][79][80][81][82][83][84].…”

Section: Plos Onementioning

confidence: 99%

PortaDrop: A portable digital microfluidic platform providing versatile opportunities for Lab-On-A-Chip applications

et al. 2020

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Electrowetting-on-dielectric is a decent technique to manipulate discrete volumes of liquid in form of droplets. In the last decade, electrowetting-on-dielectric systems, also called digital microfluidic systems, became more frequently used for a variety of applications because of their high flexibility and reconfigurability. Thus, one design can be adapted to different assays by only reprogramming. However, this flexibility can only be useful if the entire system is portable and easy to use. This paper presents the development of a portable, standalone digital microfluidic system based on a Linux-based operating system running on a Raspberry Pi, which is unique. We present "PortaDrop" exhibiting the following key features: (1) an "all-in-one box" approach, (2) a user-friendly, self-explaining graphical user interface and easy handling, (3) the ability of integrated electrochemical measurements, (4) the ease to implement additional lab equipment via Universal Serial Bus and the General Purpose Interface Bus as well as (5) a standardized experiment documentation. We propose that PortaDrop can be used to carry out experiments in different applications, where small sample volumes in the nanoliter to picoliter range need to be handled an analyzed automatically. As a first application, we present a protocol, where a droplet is consequently exchanged by droplets of another medium using passive dispensing. The exchange is monitored by electrical impedance spectroscopy. It is the first time, the media exchange caused by passive dispensing is characterized by electrochemical impedance spectroscopy. Summarizing, PortaDrop allows easy combination of fluid handling by means of electrowetting and additional sensing.

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Towards a personalized approach to aromatase inhibitor therapy: a digital microfluidic platform for rapid analysis of estradiol in core-needle-biopsies

Cited by 28 publications

References 25 publications

Design of a Hand-Held and Battery-Operated Digital Microfluidic Device Using EWOD for Lab-on-a-Chip Applications

Design of a Hand-Held and Battery-Operated Digital Microfluidic Device Using EWOD for Lab-on-a-Chip Applications

Role of Digital Microfluidics in Enabling Access to Laboratory Automation and Making Biology Programmable

PortaDrop: A portable digital microfluidic platform providing versatile opportunities for Lab-On-A-Chip applications

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