Towards densely sampled ultra-large area multi-MHz-OCT for in-vivo skin measurements beyond 1 cm2/sec

Göb, Madita; Burhan, Sazgar; Draxinger, Wolfgang; Kolb, Jan Philip; Huber, Robert

doi:10.1117/12.2616054

Cited by 2 publications

(3 citation statements)

References 3 publications

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“…The high acquisition speed of FDML-based MHZ-OCT offers numerous advantages. It provides large fields of view (FOV) within a reasonable timeframe by scanning many tens of square centimeters in seconds 30 . Moreover, it enables real-time 4D OCT applications, wherein increased speed can augment image quality and introduce vital functional contrast.…”

Section: Abbreviationsmentioning

confidence: 99%

High-resolution rectoscopy using MHz optical coherence tomography: a step towards real time 3D endoscopy

Schulte,

Göb,

Singh

et al. 2024

Sci Rep

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Colonoscopy and endoscopic ultrasound play pivotal roles in the assessment of rectal diseases, especially rectal cancer and inflammatory bowel diseases. Optical coherence tomography (OCT) offers a superior depth resolution, which is a critical factor for individualizing the therapeutic concept and evaluating the therapy response. We developed two distinct rectoscope prototypes, which were integrated into a 1300 nm MHz-OCT system constructed at our facility. The rapid rotation of the distal scanning probe at 40,000 revolutions per minute facilitates a 667 Hz OCT frame rate, enabling real-time endoscopic imaging of large areas. The performance of these OCT-rectoscopes was assessed in an ex vivo porcine colon and a post mortem human in-situ colon. The OCT-rectoscope consistently distinguished various layers of the intestinal wall, identified gut-associated lymphatic tissue, and visualized a rectal polyp during the imaging procedure with 3D-reconstruction in real time. Subsequent histological examination confirmed these findings. The body donor was preserved using an ethanol-glycerol-lysoformin-based technique for true-to-life tissue consistency. We could demonstrate that the novel MHZ-OCT-rectoscope effectively discriminates rectal wall layers and crucial tissue characteristics in a post mortem human colon in-situ. This real-time-3D-OCT holds promise as a valuable future diagnostic tool for assessing disease state and therapy response on-site in rectal diseases.

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Section: Abbreviationsmentioning

confidence: 99%

High-resolution rectoscopy using MHz optical coherence tomography: a step towards real time 3D endoscopy

Schulte,

Göb,

Singh

et al. 2024

Sci Rep

Self Cite

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“…In previous studies, multiple techniques have been utilized to expand the lateral field of view (FOV) of OCT. These approaches are distinct from expanding the lateral FOV of a single OCT scan through the use of wide-angle camera lenses [ 25 ] or the acquisition of multiple single FOVs using translational motion stages [ 26 – 28 ] or robotic arms [ 24 , 29 – 37 ] for creating a mosaic of individual scans in post. Song et al demonstrated an OCT system that could scan an approximately 200 × 200 mm 2 area in one scan by using a wide-angle camera lens and a 100 kHz swept source laser with a 500 × 500 A-Scans resolution within ∼2.5 s [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

“…To minimize sample motion, a chin- and headrest in conjunction with a bite bar are used, which may cause some discomfort for the patient. Göb et al introduced a translational stage with micrometer resolution for large-area imaging and vascular contrast imaging in combination with MHz-OCT systems [ 26 , 27 ]. The device can capture a mosaiced FOV up to 200 × 200 mm 2 using single mosaic tiles of ∼12 × 12 mm 2 or with an interplay of stage and scanner movement resulting in elongated single scans.…”

Section: Introductionmentioning

confidence: 99%

Large area robotically assisted optical coherence tomography (LARA-OCT)

Lotz,

Göb,

Böttger

et al. 2024

Biomed. Opt. Express

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We demonstrate large-area robotically assisted optical coherence tomography (LARA-OCT), utilizing a seven-degree-of-freedom robotic arm in conjunction with a 3.3 MHz swept-source OCT to raster scan samples of arbitrary shape. By combining multiple fields of view (FOV), LARA-OCT can probe a much larger area than conventional OCT. Also, nonplanar and curved surfaces like skin on arms and legs can be probed. The lenses in the LARA-OCT scanner with their normal FOV can have fewer aberrations and less complex optics compared to a single wide field design. This may be especially critical for high resolution scans. We directly use our fast MHz-OCT for tracking and stitching, making additional machine vision systems like cameras, positioning, tracking or navigation devices obsolete. This also eliminates the need for complex coordinate system registration between OCT and the machine vision system. We implemented a real time probe-to-surface control that maintains the probe alignment orthogonal to the sample by only using surface information from the OCT images. We present OCT data sets with volume sizes of 140 × 170 × 20 mm3, captured in 2.5 minutes.

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Towards densely sampled ultra-large area multi-MHz-OCT for in-vivo skin measurements beyond 1 cm2/sec

Cited by 2 publications

References 3 publications

High-resolution rectoscopy using MHz optical coherence tomography: a step towards real time 3D endoscopy

High-resolution rectoscopy using MHz optical coherence tomography: a step towards real time 3D endoscopy

Large area robotically assisted optical coherence tomography (LARA-OCT)

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