Towards the Transcriptionally based Classification of L6b in the Adult Mouse Brain

Henning, Thomas; Bandiera, Sara; Molnár, Zoltán

doi:10.1002/9781119860914.ch16

Cited by 2 publications

(2 citation statements)

References 53 publications

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“…However, this overlap may differ among the sensory cortices and likely represent only a small population in ACtx, since only ∼8% of the total SpNs population in Drd1a‐Cre mice was expressing Cplx3. Moreover, future studies using single‐cell transcriptomic analysis might aid in dissecting the relationship between these subclasses (Henning et al., 2023).…”

Section: Discussionmentioning

confidence: 99%

Distinct distribution of subplate neuron subtypes between the sensory cortices during the early postnatal period

Chang,

Nehs,

et al. 2024

J of Comparative Neurology

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Subplate neurons (SpNs) are a heterogeneous neuronal population actively involved in early cortical circuit formation. In rodents, many SpNs survive and form layer 6b. The molecular heterogeneity of SpNs raises the question of whether different subpopulations of SpNs survive through the early postnatal period similarly and whether such diverse SpN populations in the auditory cortex (ACtx) share a common distribution pattern with other sensory systems. To address that, we investigated the expression pattern of multiple specific SpN markers in the ACtx, as well as in the visual (VCtx) and somatosensory (SCtx) cortices as controls, using complexin 3 (Cplx3) antibodies and different SpN‐specific Cre‐driver mice, such as connective tissue growth factor (CTGF), dopamine receptor D1 (Drd1a), and neurexophilin 4 (Nxph4). We focused on two early time windows in auditory development: (1) during the second postnatal week (PNW) before ear‐canal opening and (2) during the third PNW after ear‐canal opening. We compared the expression pattern of different SpN markers in ACtx with VCtx and SCtx. At both examined timepoints, Cplx3 and Nxph4 expressing SpNs form the largest and smallest population in the ACtx, respectively. Similar distribution patterns are observable in the VCtx and SCtx during the second PNW but not during the third PNW, for a higher proportion of Drd1a expressing SpNs is detected in the VCtx and CTGF expressing SpNs in the SCtx. This study suggests that different populations of SpNs might contribute differently to the development of individual sensory circuits.

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Section: Discussionmentioning

confidence: 99%

Distinct distribution of subplate neuron subtypes between the sensory cortices during the early postnatal period

Chang,

Nehs,

et al. 2024

J of Comparative Neurology

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“…Recordings from Drd1a-Cre-L6 neurons showed that YNT-185 only reduced the spike frequency at higher current steps (Figure 1c-g). Recent studies in the transcriptomic factors of layer 6 neurons have shown that subclasses of both excitatory and inhibitory L6 neurons can express orexin receptors (Henning et al, 2023), and thus the reduction in Drd1a-Cre-neuron firing rate could be due to the activation of L6 inhibitory neurons. While Drd1a-Cre+ L6 neurons may not be uniquely sensitive to orexin in mPFC, these results are consistent with a role for Drd1a-Cre+ L6 neurons in mediating orexin-induced increases in cortical excitability across sensorimotor cortex and mPFC.…”

mentioning

confidence: 99%

An orexin-sensitive subpopulation of layer 6 neurons regulates cortical excitability and anxiety behaviour

Messore,

Therpurakal,

Dufour

et al. 2024

Preprint

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Cortical layer 6 (L6) neurons uniquely respond to orexin, a neuropeptide influencing arousal and emotion. We show that Drd1a-Cre+ neurons in the prefrontal cortex are selectively sensitive to orexin and regulate prefrontal network activation in vitro and in vivo. Chronically silencing these neurons impairs orexin-induced prefrontal activation and reduces anxiety-like behaviour, indicating that orexin-responsive L6 neurons modulate emotional states and may be a substrate for anxiety regulation.

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Towards the Transcriptionally based Classification of L6b in the Adult Mouse Brain

Cited by 2 publications

References 53 publications

Distinct distribution of subplate neuron subtypes between the sensory cortices during the early postnatal period

Distinct distribution of subplate neuron subtypes between the sensory cortices during the early postnatal period

An orexin-sensitive subpopulation of layer 6 neurons regulates cortical excitability and anxiety behaviour

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