Toxic and DNA damaging effects of a functionalized fullerene in human embryonic lung fibroblasts

Ershova, Elizaveta S.; Sergeeva, Vasilina A.; Chausheva, A. I.; Zheglo, Diana; Nikitina, V. A.; Смирнова, Т. Д.; Kameneva, Larisa V.; Porokhovnik, Lev N.; Куцев, С. И.; Troshin, Pavel A.; Voronov, Ilya I.; Khakina, Ekaterina A.; Вейко, Н. Н.; Kostyuk, Svetlana V.

doi:10.1016/j.mrgentox.2016.05.004

Cited by 35 publications

(35 citation statements)

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“…C 60 fullerenes and their derivatives stimulate oxidative metabolism in erythrocytes and fibroblasts, malignant lymphocytes and epithelial cells, and endothelial cells and macrophages (Trpkovic et al 2012 ). Ershova et al ( 2016 ) reported about complex time-dependent changes in ROS levels in cells treated with C 60 fullerene derivatives. They differentiate between “early” (from 15 min to 3 h) and “late” (24 h) responses to C 60 fullerene exposure.…”

Section: Resultsmentioning

confidence: 99%

“…This results in a fusion between the MLLT10 (myeloid/lymphoid or mixed-lineage leukemia) gene and the Ap-3-like clathrin assembly protein PICALM (Clathrin assembly lymphoid myeloid leukemia), which is likely important for the tumorous nature of the cell line. Treatment exposure was 24 h. It suggests that the effect of nanoformulation could be mediated through the interaction with both membrane-associated and intracellular receptive structures (Ershova et al 2016 ). Intracellular ROS levels in U937 cells, as indicated by fluorescence intensity, significantly increased in response to the treatment with C 60 fullerenes and their nanocomplexes with anticancer drugs (Fig.…”

Section: Resultsmentioning

confidence: 99%

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C60 fullerene and its nanocomplexes with anticancer drugs modulate circulating phagocyte functions and dramatically increase ROS generation in transformed monocytes

et al. 2018

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BackgroundC60 fullerene-based nanoformulations are proposed to have a direct toxic effect on tumor cells. Previous investigations demonstrated that C60 fullerene used alone or being conjugated with chemotherapeutic agents possesses a potent anticancer activity. The main aim of this study was to investigate the effect of C60 fullerene and its nanocomplexes with anticancer drugs on human phagocyte metabolic profile in vitro.MethodsAnalysis of the metabolic profile of phagocytes exposed to C60 fullerene in vitro revealed augmented phagocytic activity and down-regulated reactive nitrogen species generation in these cells. Additionally, cytofluorimetric analysis showed that C60 fullerene can exert direct cytotoxic effect on normal and transformed phagocytes through the vigorous induction of intracellular reactive oxygen species generation.ResultsCytotoxic action as well as the pro-oxidant effect of C60 fullerene was more pronounced toward malignant phagocytes. At the same time, C60 fullerenes have the ability to down-regulate the pro-oxidant effect of cisplatin on normal cells. These results indicate that C60 fullerenes may influence phagocyte metabolism and have both pro-oxidant and antioxidant properties.ConclusionsThe antineoplastic effect of C60 fullerene has been observed by direct toxic effect on tumor cells, as well as through the modulation of the functions of effector cells of antitumor immunity.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

C60 fullerene and its nanocomplexes with anticancer drugs modulate circulating phagocyte functions and dramatically increase ROS generation in transformed monocytes

et al. 2018

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“…Non-thermal DBD plasma treatment induces oxidative stress from the diffusion of plasma-generated ROS, or from stimulation of the cell’s own ROS generating mechanisms [ 32 ], which results in decreased cell proliferation and differentiation [ 31 ], even cell death [ 29 , 30 , 32 ]. Our results show 4 min plasma exposure generated excessive ROS and increased MDA activity in dead embryos, which were mediated by decreasing cellular antioxidant activity and metabolic viability [ 20 , 33 ], and correlated with elevated NOX4 mRNA expression, which catalyzes the reduction of molecular oxygen to generate ROS [ 34 – 36 ]. NRF2 directly affects ROS levels by regulating the antioxidant defense systems through the induction of catabolism in SOD, CAT, GPx, and PRDX [ 26 , 28 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

Lethality of inappropriate plasma exposure on chicken embryonic development

Zhang

Huynh

et al. 2017

Oncotarget

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In this study, we examined the effects of non-thermal dielectric barrier discharge plasma on embryonic development in chicken eggs in order to determine the optimal level of plasma exposure for the promotion of embryonic growth. We exposed developing chicken embryos at either Hamburger-Hamilton (HH) stage 04 or HH 20 to plasma at voltages of 11.7 kV to 27.6 kV. Our results show exposure at 11.7 kV for 1 min promoted chicken embryonic development, but exposure to more duration and intensity of plasma resulted in dose-dependent embryonic death and HH 20 stage embryos survive longer than those at stage HH 04. Furthermore, plasma exposure for 4 min increased the production of reactive oxygen species (ROS) and inactivated the nuclear factor erythroid 2-related factor 2 (NRF2)-antioxidant response signaling pathway, resulting in suppression of antioxidant enzymes in the skeletal muscle tissue of the dead embryos. We also found decreased levels of adenosine triphosphate production and reductions in the expression levels of several growth-related genes and proteins. These findings indicate that inappropriate plasma exposure causes dose-dependent embryonic death via excessive accumulation of ROS, NRF2-antioxidant signaling pathway disruption, and decreased growth factor expression.

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“…Ershova et al . 81 studied the cytotoxicity and genotoxicity of a C 60 fullerene derivative functionalized with five residues of 3-phenylpropionic acid and a chlorine atom arranged around one cyclopentadienyl unit, compound 36 (Fig. 7), in diploid human embryonic lung fibroblasts (HELFs).…”

Section: Photodynamic Therapymentioning

confidence: 99%