Toxic effects of copper sulphate pentahydrate on antioxidant enzyme activities and lipid peroxidation of freshwater fish Capoeta umbla (Heckel, 1843) tissues

Abstract

Abstract. Copper sulphate pentahydrate (CuSO 4 .5H 2 O) is commonly used as a general biocide in the agricultural fields surrounding freshwater reservoirs. So the aim of the present study was to investigate the toxic effects of CuSO 4 .5H 2 O by examining oxidative damage markers such as malondialdehyde (MDA) for lipid peroxidation and the antioxidant enzymatic defense system components (glutathione reductase (GR), glucose 6-phosphate dehydrogenase (G6PD), superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT)) in freshwater fish Capoeta umbla (Heckel, 1843) liver, kidney and gill. In this purpose, the 96 h LC 50 values (with 95% confidence limits) of CuSO 4 .5H 2 O for C. umbla was estimated as a 1.478 mg/L. Three different CuSO 4 .5H 2 O sublethal doses (0.185, 0.370 and 0.739 mg/L) were administered to C. umbla at different time intervals (12, 24, 48, 72 and 96 h). Antioxidant enzyme activities and MDA levels were measured spectrophotometrically. Liver, kidney and gills samples were collected at the end of the experiment, and analysed for their oxidant-antioxidant status, including MDA level, GR, G6PD, SOD, CAT and GPx activities. Results obtained showed that CuSO 4 .5H 2 O, significantly decreased (P<0.05) the GR, G6PD and GPx activities, but increased (P<0.05) the CAT, SOD activities and MDA levels of C. umbla liver, kidney and gill tissue in a dose-dependent manner. However, CuSO 4 .5H 2 O exposure did not cause to changes significantly (P<0.05) in enzyme activities and MDA levels of C. umbla liver, kidney and gill tissue in a time-dependent manner.

Determination of Some Metal Levels in Water, Sediment and Fish Species of Tercan Dam Lake, Turkey

“…Numerous studies have been conducted on the accumulation of metals in water, sediments, and fish (Moiseenko and Kudryavtseva, 2001 Kirici et al, 2013aKirici et al, , 2016aKirici et al, , 2017b. In this study, it was aimed at determining the metal (Al, As, Cr, Cu, Fe, Mn, Ni, Pb, and Zn) concentrations in the gills, livers, and muscle tissues of Grass carp (Ctenopharyngodon idella), Caner fish (Barbus capito), Common carp (Cyprinus carpio) and Tigris scraper (Capoeta umbla), which live in the Tercan Dam Lake-within the borders of Erzincan provincewater, sediment, and lake; it was also aimed at determining the relationship between metal levels in the water and the measured certain physico-chemical parameters of the water.…”

Hematological Abnormality, Oxidative Stress, and Genotoxicity Induction in the Greenhouse Pesticide Sprayers; Investigating the Role of NQO1 Gene Polymorphism

“…In recent years, increasing attention has been given to the assessment of biomarkers of human exposure to pesticides [ 7 ]. Pesticides may induce oxidative stress, leading to the generation of free radicals, accumulation of reactive oxygen species (ROS), alteration in the scavenging enzyme system, and depletion of cellular antioxidant reservoirs, finally resulting in damage to all components of the cell, including lipids, proteins, and DNA [ 8 ].…”

Biochemical and Histopathological Examination of Changes in Infected Capoeta trutta (Heckel, 1843) with Neoechinorhynchus rutili (Acanthocephala)

“…Bu enzimler aktif oksijen türlerini ortadan kaldırmak için beraber çalışırlar ve fizyolojik konsantrasyonlarda aktivitelerinin veya miktarlarının azalması sonucu hücresel lipidlerin, proteinlerin ve DNA'nın oksidatif hasardan olumsuz etkilenmelerine neden olurlar [27]. Bir diğer antioksidan enzim olan GPx, substrat olarak kullandığı GSH varlığında H 2 O 2 'in su ve moleküler oksijene dönüşümünü katalize eder [26]. Yapılan çalışmada 1. grup ile karşılaştırıldığında 2. grupta antioksidan enzimler olan SOD, KAT ve GPx aktivitelerinin oldukça düşük olduğu belirlenmiştir.…”

“…Bu bağlamda SOD enzimi süperoksit radikalini moleküler oksijene ve H 2 O 2 'e dönüştürür. KAT enzimi ise H 2 O 2 gibi zararlı bir bileşiği parçalayıp, moleküler oksijene ve suya dönüştürerek oksidatif hasarın derecesini azaltır [26]. Bu enzimler aktif oksijen türlerini ortadan kaldırmak için beraber çalışırlar ve fizyolojik konsantrasyonlarda aktivitelerinin veya miktarlarının azalması sonucu hücresel lipidlerin, proteinlerin ve DNA'nın oksidatif hasardan olumsuz etkilenmelerine neden olurlar [27].…”