Toxic Effects of Methotrexate on Rat Kidney Recovered by Crocin as a Consequence of Antioxidant Activity and Lipid Peroxidation Prevention

Jalili, Cyrus; Ghanbari, Ali; Roshankhah, Shiva; Salahshoor, Mohammad Reza

doi:10.29252/ibj.24.1.39

Cited by 27 publications

(18 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 4 MTX cytotoxicity, as with other chemotherapeutic agents, was reported to affect many organs including liver, kidney, and testis in both clinical and experimental studies. 5 - 8 …”

Section: Introductionmentioning

confidence: 99%

“…Many studies discussed the role of natural antioxidants in ameliorating MTX side effects. 6 - 8 ,14 One of the traditional Chinese medicines that was utilized in the management and treatment of different diseases is Ginkgo biloba . 23 Terpene lactones and flavone glycosides are the ingredients of the Ginkgo biloba standardized extract (GIN).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ginkgo biloba Extract Attenuates Methotrexate-Induced Testicular Injury in Rats: Cross-talk Between Oxidative Stress, Inflammation, Apoptosis, and miRNA-29a Expression

2020

View full text Add to dashboard Cite

Ginkgo biloba leaf extract (GIN) is a popular Chinese herbal medicine. It has a nephroprotective effect against the nephrotoxicity induced by the chemotherapeutic agent methotrexate (MTX). This work was designed to explore the testicular protective role of GIN on MTX-induced testicular injury in a rat model. The experimental protocol lasted for 10 days for the 4 studied groups. First group: received saline (normal control, NC group). The second group was administered GIN (100 mg/kg/day) orally for 10 days (GIN C). Third group: injected with MTX (20 mg/kg ip) only on the fifth day (MTX group). Fourth group: administered GIN for 10 days with MTX injection on the fifth day (GIN+MTX group). MTX induced testicular injury as evident by a marked rise in the malondialdehyde (MDA) content, interleukin-6 (IL-6) and IL-1β protein levels, nuclear factor kappa-B (NF-κB) protein expression, bcl-2 associated × protein (Bax) mRNA expression, p53 mRNA and protein expressions, and miRNA29-a expression along with a marked decline in the serum level of testosterone and superoxide dismutase (SOD) content in testicular tissue in relation to the NC group. Moreover, histopathological testicular damage with a notable decrease in the Johnsen score together with a significant elevation in the testicular injury score was observed in the MTX group in comparison to the NC group. The administration of GIN ameliorated the biochemical changes as well as the testicular histopathological findings and scores. GIN could protect against MTX-induced gonadotoxicity by its antioxidant, anti-inflammatory, antiapoptotic activities plus the regulation of the miRNA-29a testicular expression.

show abstract

“… 4 MTX cytotoxicity, as with other chemotherapeutic agents, was reported to affect many organs including liver, kidney, and testis in both clinical and experimental studies. 5 - 8 …”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ginkgo biloba Extract Attenuates Methotrexate-Induced Testicular Injury in Rats: Cross-talk Between Oxidative Stress, Inflammation, Apoptosis, and miRNA-29a Expression

2020

View full text Add to dashboard Cite

show abstract

“…Crocin supplementation has also been shown to ameliorate the renal oxidant/antioxidant imbalance induced by advancing age in rats [ 14 ]. Finally, by preserving the oxidant-antioxidant balance, crocin prevented methotrexate-induced renal damage [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

“…Crocin treatment also reduced nephropathy in other pathological settings, including gentamicin-induced nephrotoxicity [ 19 ], diabetes [ 20 ], carbon tetrachloride nephropathy [ 21 ], and methotrexate nephropathy [ 22 ]. The reported anti-inflammatory properties of crocin included reducing the abundance of COX2 and TNF α mRNA as well as iNOS expression and nitric oxide production via downregulation of NF- κ B activity [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Anti‐Inflammatory Effects of Crocin Ameliorate Doxorubicin‐Induced Nephrotoxicity in Rats

Hussain

Abogresha

Kader

et al. 2021

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Doxorubicin is a drug that belongs to the anthracycline antibiotics. Nephrotoxicity is one of the serious side effects of doxorubicin treatment. Crocin, which is one of the most bioactive components of saffron, has antioxidant, anti-inflammatory, and antitumor effects. The current study was aimed at investigating the possible protective effects of crocin against doxorubicin-induced nephrotoxicity to elucidate the underlying mechanism of this effect. The study included four groups, six rats in each group: normal control, crocin control, doxorubicin, and crocin/doxorubicin. Doxorubicin and crocin/doxorubicin groups received intraperitoneal injections of doxorubicin (3.5 mg/kg twice weekly for 3 weeks). Rats in the crocin control group and the crocin/doxorubicin group were treated with intraperitoneal injections of crocin (100 mg/kg body weight per day) for 3 weeks. Biomarkers of kidney function and oxidative stress as well as the abundance of mRNA for nuclear factor-κβ and inducible nitric oxide synthase were evaluated. In addition, the abundance of cyclooxygenase 2 and tumor necrosis factor α immunoreactivity was evaluated. Crocin treatment had renoprotective effects manifested by significant improvement in kidney function as well as a reduction in the abundance of biomarkers of oxidative stress markers and inflammatory mediators. In conclusion, crocin has a protective effect against doxorubicin-induced nephrotoxicity in rats by serving as an antioxidant and attenuating the expression of NF-κB, iNOS, COX2, and TNFα.

show abstract

“…The serum samples were analyzed for Scr and BUN using an autoanalyzer (cobas c 701, Roche) 15 . The diameter of the renal pelvis in rats was performed by a Doppler ultrasound diagnostic instrument (HD3, Philips) under sodium pentobarbital (30 mg/kg, intraperitoneal injection) anesthesia.…”

Section: Methodsmentioning

confidence: 99%

Losartan prevents bladder fibrosis and protects renal function in rat with neurogenic paralysis bladder

Wen

et al. 2021

Neurourology and Urodynamics

View full text Add to dashboard Cite

Aims: To investigate the effect of losartan on preventing bladder fibrosis and protecting renal function in rats with neurogenic paralysis bladder (NPB). Materials and Methods: Rats were assigned to the transecting spinal nerves group (TSNG), transecting spinal nerves + losartan group (LSTG), and control group (CG). On Day 32 postsurgery, bladder capacity (BC), bladder compliance (ΔC), bladder leakage pressure (P ves.leak) of TSNG and LSTG while BC, ΔC, and bladder threshold pressure (P ves.thre) of CG were measured by cystometry in each cohort. Renal function and the expression quantity of Angiotensin Ⅱ (Ang II) in blood were detected, in addition Ang II, Ang II Type 1 receptor (AT1), transformation growth factor β1 (TGFβ1), Collagen Ⅲ, and collagen fibrin in the bladder tissue were detected too. Results: ΔC in TSNG and LSTG decreased significantly compared to the CG. P ves.leak in TSNG and LSTG were significantly higher than P ves.thre in CG. Renal function of both TSNG and LSTG decreased significantly compared with the CG, but renal function in LSTG was better than in TSNG. Ang Ⅱ in blood and bladder tissue in TSNG and LSTG increased significantly compared with CG. AT1 was expressed in the bladder tissue of all rats. The TGFβ1, Collagen Ⅲ, and collagen fibrin expression level increased significantly in TSNG compared with LSTG and CG, while these levels were not significantly different between CG and LSTG. Conclusion: Losartan might prevent NPB fibrosis by stopping the upregulated signaling of Ang II/AT1/TGFβ1 and consequently may reduce kidney damage from occurring.

show abstract

Toxic Effects of Methotrexate on Rat Kidney Recovered by Crocin as a Consequence of Antioxidant Activity and Lipid Peroxidation Prevention

Cited by 27 publications

References 27 publications

Ginkgo biloba Extract Attenuates Methotrexate-Induced Testicular Injury in Rats: Cross-talk Between Oxidative Stress, Inflammation, Apoptosis, and miRNA-29a Expression

Ginkgo biloba Extract Attenuates Methotrexate-Induced Testicular Injury in Rats: Cross-talk Between Oxidative Stress, Inflammation, Apoptosis, and miRNA-29a Expression

Antioxidant and Anti‐Inflammatory Effects of Crocin Ameliorate Doxorubicin‐Induced Nephrotoxicity in Rats

Losartan prevents bladder fibrosis and protects renal function in rat with neurogenic paralysis bladder

Contact Info

Product

Resources

About