Toxic Effects Produced or Mediated by Human Eosinophil Granule Components on Trypanosoma cruzi

Molina, Hugo A.; Kierszenbaum, Felipe; Hamann, Kimm J.; Gleich, Gerald J.

doi:10.4269/ajtmh.1988.38.327

Cited by 72 publications

(49 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Yet both forms were equally effective at reducing the number of colony forming units of a characterized target strain of Staphylococcus aureus (35). Similar results were obtained by Molina and colleagues (36) in their study of the anti-helminth activity of native ECP. The results of this work--that ribonuclease activity was not essential for cytotoxicity--was not only surprising but counterintuitive from an evolutionary perspective.…”

Section: Ribonuclease Activity and Cytotoxicitysupporting

confidence: 79%

Eosinophils, ribonucleases and host defense: Solving the puzzle

Rosenberg

Domachowske

1999

Immunol Res

View full text Add to dashboard Cite

The eosinophil ribonucleases eosinophil-derived neurotoxin (EDN/ RNase 2) and eosinophil cationic protein (ECP/RNase 3) are among the major secretory effector proteins of human eosinophilic leukocytes, cells whose role in host defense remains controversial and poorly understood. We have recently described the unusual manner in which this ribonuclease lineage has evolved, with extraordinary diversification observed in primate as well as in rodent EDNs and ECPs. The results of our evolutionary studies suggest that the EDN/ ECP ribonucleases are in the process of being tailored for a specific, ribonuclease-related goal. With this in mind, we have begun to look carefully at some of the intriguing associations that link eosinophils and their ribonucleases to disease caused by the single-stranded RNA viral pathogen, respiratory syncytial virus (RSV). Recent work in our laboratory has demonstrated that eosinophils can mediate a direct, ribonuclease-dependent reduction in infectivity of RSV in vitro, and that EDN can function alone as an independent antiviral agent. The results of this work have led us to consider the possibility that the EDN/ECP ribonucleases represent a heretofore unrecognized element of innate and specific antiviral host defense.

show abstract

Section: Ribonuclease Activity and Cytotoxicitysupporting

confidence: 79%

Eosinophils, ribonucleases and host defense: Solving the puzzle

Rosenberg

Domachowske

1999

Immunol Res

View full text Add to dashboard Cite

show abstract

“…Eosinophils and their granule proteins have long been associated with defense against parasites such as S. mansoni, 8,9 Trichinella spiralis, 10 T. cruzi, 11,12 and O. volvulus. 13 Previous studies on the pathology of the P. brasiliensis granuloma have shown several different effector cells: natural killer cells, lymphocytes, cytotoxic T cells, activated neutrophils, activated macrophages, epithelioid cells, giant cells, and B cells.…”

Section: Discussionmentioning

confidence: 99%

“…[5][6][7] Eosinophils are considered to be effector cells, which in the presence of antibody or complement, are able to kill parasites. Eosinophils have been associated with defense against parasites, such as Schistosoma mansoni, 8,9 Trichinella spiralis, 10 Trypanosoma cruzi, 11,12 and Onchocerca volvulus. 13 The role of eosinophils in the immune response to fungal infections has not been extensively studied.…”

mentioning

confidence: 99%

Localization of eosinophil granule major basic protein in paracoccidioidomycosis lesions.

Wagner

Franco

Kephart

et al. 1998

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. Paracoccidioidomycosis is a chronic granulomatous disease caused by the fungus Paracoccidioides brasiliensis. Although eosinophils have long been associated with the immune defense against helminths, the role of eosinophils in the immune response to fungal diseases is not as well studied. The eosinophil granule major basic protein is toxic to helminths and mammalian cells in vitro, and its release has been used as a marker of eosinophil localization and degranulation. To determine whether eosinophil infiltration and degranulation, as evidenced by the deposition of major basic protein, occur in lesions of P. brasiliensis, we used an immunofluorescence technique to localize the P. brasiliensis organisms and eosinophils and major basic protein. Initially, all tissues were stained with polyclonal antibody to major basic protein; subsequently, colocalization of major basic protein and P. brasiliensis by double staining with mouse and rabbit antibodies, respectively, was performed. Nine biopsy tissues from seven patients were analyzed. All nine biopsies showed infiltration of intact eosinophils using both the monoclonal and the polyclonal anti-major basic protein antibodies, along with the presence of P. brasiliensis. Furthermore, using the polyclonal antimajor basic protein antibody, nine of nine tissues showed extracellular major basic protein deposition (granular or diffuse fluorescence staining outside of intact eosinophils). The double staining procedure using the anti-major basic protein monoclonal antibody showed extracellular deposition in five of eight biopsies; in these five biopsies, approximately 60% of the areas containing P. brasiliensis had extracellular major basic protein deposited on the organisms. These observations support the hypothesis that the eosinophil, through toxic granule proteins such as major basic protein, participates in the pathophysiology of paracoccidioidomycosis.

show abstract

“…1d, e) [30]. Eosinophils are considered to be effector cells, which in the presence of antibodies or complement, are able to kill parasites [41][42][43][44][45][46]. The role of eosinophils in the immune response to fungal infections has not been extensively studied.…”

Section: Inflammatory Cellsmentioning

confidence: 99%