Toxicity evaluation of selected ionic liquid compounds on embryonic development of Zebrafish

Younes, Nadin; Salem, Rola; Al-Asmakh, Maha; Altamash, Tausif; Pintus, Gianfranco; Khraisheh, Majeda; Nasrallah, Gheyath K.

doi:10.1016/j.ecoenv.2018.05.064

Cited by 39 publications

(43 citation statements)

References 47 publications

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“…Two compounds were employed as positive controls for general toxicity: diethylaminobenzaldehyde (DEAB, Sigma-Aldrich, Steinheim, Germany), an inhibitor of aldehyde dehydrogenases that causes signi cant pathologies and mortality in zebra sh embryos [25][26][27] and nanoparticles of zinc oxide (ZnO, diameter < 100 nm, catalog #721077-100G, Sigma-Aldrich, Steinheim, Germany) known to cause mortality and morphological deformities in zebra sh embryos and used previously as a positive control in toxicological studies [28][29][30]. In addition, in connection with the assays of neurotoxicity, 1-methyl-4phenyl-1,2,3,6-tetrahydropyridinehydrochloride (MPTP, Sigma-Aldrich, Steinheim, Germany), which causes permanent symptoms of Parkinson's disease in these same embryos, was used as the positive control [31].…”

Section: Chemicalsmentioning

confidence: 99%

“…A stock solution of AEO-7 (2 mg/L) was prepared by adding 5 L of the viscous liquid to 4.995 mL PTU medium and overtaxing until fully dissolved. Stock solutions of DEAB, ZnO, PTU, E3 egg water, and phosphate buffer saline (PBS) were prepared as described previously [2,25].…”

Section: Chemicalsmentioning

confidence: 99%

“…Since different organs of the zebra sh become fully functional at different stages [26], acute toxicity, cardiotoxicity, and hematopoiesis were assessed once every 24 h for three days after initiating exposure at 24 hpf, and central nervous system (CNS) toxicity and hepatotoxicity following treatment from 96-120 hpf, as illustrated in Fig 1 [24,25,27,[35][36][37][38][39]. During early embryogenesis the protective chorion envelope around the embryos might interfere with uptake of the compounds being tested.…”

Section: Zebra Sh Embryo Culturementioning

confidence: 99%

“…The median lethal dose (LC 50 ) with a 95% con dence interval was calculated by tting a sigmoidal curve to the data on mortality using the GraphPad Prism 8 software (version 8.2.1,San Diego, CA, USA), as described elsewhere [25,39,42]. Variations in body length and the size of the eyes and yolk sac size were captured at 21-fold magni cation with the HCImage software and then assessed with the ImageJ software (version 1.52a, NIH, Washington DC, USA) in combination with Java 1.8.0_172 [25,43].…”

Section: Acute Toxicitymentioning

confidence: 99%

“…Accordingly, the present study was designed to evaluate the potential toxic effects of AEO-7 using zebra sh embryos as a model for the marine fauna. Zebra sh (Danio rerio) is an ideal model system to study environmental toxicity and is accepted by the National environmental toxicity and by the National Institutes of Health (NIH, USA) as an alternative model for exploring human diseases [2,[23][24][25]. Since no toxicity studies have been performed on AEO-7, we investigated a wide range of concentrations (0.4, 0.8, 3.2, 6.4, 12.8, 25, µg/L) to assess the no observed effect concentration (NOEC) and the median lethal concentration (LC50).…”

Section: Introductionmentioning

confidence: 99%

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AEO-7 Surfactant is “super Toxic” and Induces Severe Cardiac, Liver and Locomotion Damage in Zebrafish Embryos

Al-Asmakh

Majdalawieh

Abdullah

et al. 2020

Preprint

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BackgroundFatty alcohol polyoxyethylene ether-7 (AEO-7), a non-ionic surfactant, has recently been receiving extensive attention from the ocean pipeline industry for its ability to inhibit corrosion. However, the present lack of information concerning the potential environmental toxicity of AEO-7, especially towards aquatic organisms, is a major impediment to its wider application. Here, we assess potential adverse effects of AEO-7 on zebrafish embryos employing a variety of assays, including (i) a mortality/survival assay which allowed the median lethal concentration (LC50) to be calculated; (ii) a teratogenicity assay on the basis of which the no observed effect concentration (NOEC) was determined; and (iii) specific assays of cardiotoxicity, neurotoxicity (based on locomotion), hematopoietic toxicity (the level of hemoglobin as revealed by o-dianisidine staining) and hepatotoxicity (liver steatosis and yolk retention examined by staining with Oil Red O). ResultsAEO-7 caused mortality with a calculated LC50 of 15.35 μg/L, which, according to the U.S. Fish and Wildlife Service (USFWS) Acute Toxicity Rating scale, should be considered “super toxic”. Although at its NOEC (0.8 μg/L), there were no signs of significant teratogenicity, cardiotoxicity, or hemopoiesis toxicity, 3.2 µg/L AEO-7 exerted dramatic detrimental effects on organ development. ConclusionOn the basis of these findings, we recommend that the industrial usage and environmental impact of AEO-7 be re-evaluated and strictly monitored by environmental and public health organizations.

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Section: Chemicalsmentioning

confidence: 99%

Section: Chemicalsmentioning

confidence: 99%

Section: Zebra Sh Embryo Culturementioning

confidence: 99%

Section: Acute Toxicitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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AEO-7 Surfactant is “super Toxic” and Induces Severe Cardiac, Liver and Locomotion Damage in Zebrafish Embryos

Al-Asmakh

Majdalawieh

Abdullah

et al. 2020

Preprint

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Integration of choline geranate into electrospun protein scaffolds affords antimicrobial activity to biomaterials used for cutaneous wound healing

et al. 2020

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Wound healing attempts to maintain homeostasis in the wound while minimizing the risk of infection to the tissue by foreign agents, such as opportunistic bacterial pathogens. Biofilms established by these pathogens are a common cause of chronic infections that slow the healing process. Preparation of skin wound healing devices comprised of electrospun proteins associated with skin have been shown to accelerate the healing process relative to conventional wound dressings. In this work, we have developed electrospinning methods to incorporate the antimicrobial ionic liquid/deep eutectic solvent choline geranate (CAGE) into these devices. Integration of CAGE into the dressing material was verified via 1 H nuclear magnetic resonance spectrometry, and the effect on the material property of the resultant devices were assessed using scanning electron microscopy. CAGE-containing devices demonstrate a concentration-dependent inactivation of exogenously applied solutions of both gram-positive and gram-negative pathogens (Enterococcus sp and Pseudomonas aeruginosa, respectively), but maintain their ability to serve as a compatible platform for proliferation of human dermal neonatal fibroblasts.

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Synthesis and Optimization of a Highly Stable and Efficient BN/TiO₂ Nanocomposite for Phenol Degradation: A Photocatalytic, Mechanistic and Environmental Impact Study

et al. 2021

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Different BN/TiO 2 nanocomposites were prepared hydrothermally, and their ratio was optimized to get the best photocatalytic performance towards phenol degradation. They were characterized by x-ray photoelectron spectroscopy, x-ray diffraction, Fourier transform infrared spectroscopy, thermal gravimetric analysis, scanning and transmission electron microscopies coupled with energy dispersive x-ray units, BET surface area, and UV-Vis diffuse reflectance. The bandgap energy was reduced from 3.35 to 2.95 eV due to the formation of the BÀ OÀ Ti bond. This allowed the exploitation of the visible light and inhibited the TiO 2 e À /h + recombination, and consequently, the photocatalytic activity of TiO 2 was dramatically improved. Almost 90 % mineralization of 20 ppm phenol solution was achieved within 30 min under simulated sunlight. The as-prepared composite showed excellent stability and reusability. Mechanistic analysis indicated that O : À 2 and h + played a crucial role in phenol degradation. The nanocomposite's biocompatibility and environmental impact were evaluated by analyzing its potential toxicity in vivo using the zebrafish embryos. 96-hpf acute toxicity assays, including the mortality rate assay (to obtain the LC50 values) and teratogenic assays (to obtain the No Observed Effect Concentration, NOEC) was conducted. The LC50 value for BN/TiO 2 was 482.5 mg L À 1 , and the NOEC was 100 mg L À 1 . Based on LC50 value and according to the Fish and Wildlife Service (FWS) acute toxicity rating scale, the photocatalyst is "practically not toxic."

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Toxicity evaluation of selected ionic liquid compounds on embryonic development of Zebrafish

Cited by 39 publications

References 47 publications

AEO-7 Surfactant is “super Toxic” and Induces Severe Cardiac, Liver and Locomotion Damage in Zebrafish Embryos

AEO-7 Surfactant is “super Toxic” and Induces Severe Cardiac, Liver and Locomotion Damage in Zebrafish Embryos

Integration of choline geranate into electrospun protein scaffolds affords antimicrobial activity to biomaterials used for cutaneous wound healing

Synthesis and Optimization of a Highly Stable and Efficient BN/TiO₂ Nanocomposite for Phenol Degradation: A Photocatalytic, Mechanistic and Environmental Impact Study

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Toxicity evaluation of selected ionic liquid compounds on embryonic development of Zebrafish

Cited by 39 publications

References 47 publications

AEO-7 Surfactant is “super Toxic” and Induces Severe Cardiac, Liver and Locomotion Damage in Zebrafish Embryos

AEO-7 Surfactant is “super Toxic” and Induces Severe Cardiac, Liver and Locomotion Damage in Zebrafish Embryos

Integration of choline geranate into electrospun protein scaffolds affords antimicrobial activity to biomaterials used for cutaneous wound healing

Synthesis and Optimization of a Highly Stable and Efficient BN/TiO2 Nanocomposite for Phenol Degradation: A Photocatalytic, Mechanistic and Environmental Impact Study

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Product

Resources

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Synthesis and Optimization of a Highly Stable and Efficient BN/TiO₂ Nanocomposite for Phenol Degradation: A Photocatalytic, Mechanistic and Environmental Impact Study