Toxicity, genotoxicity and proinflammatory effects of amorphous nanosilica in the human intestinal Caco-2 cell line

Tarantini, Adeline; Lanceleur, Rachelle; Mourot, Annick; Lavault, Marie Thérèse; Casterou, Gérald; Jarry, Gérard; Hogeveen, Kevin; Fessard, Valérie

doi:10.1016/j.tiv.2014.10.023

Cited by 83 publications

(57 citation statements)

References 68 publications

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“…In addition, SiNPs addition to culture media significantly increased cellular ROS production and intracellular Ca 2+ accumulation30. Besides direct cytotoxicity, Tarantini et al 31. tested 15 nm and 55 nm sized SiNPs in human intestinal cell lines and observed significantly increased secretion of inflammatory cytokines by the cells.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Silica Nanoparticles on Human Corneal Epithelial Cells

Park

Jeong

Hong

et al. 2016

Sci Rep

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Ocular drug delivery is an interesting field in current research. Silica nanoparticles (SiNPs) are promising drug carriers for ophthalmic drug delivery. However, little is known about the toxicity of SiNPs on ocular surface cells such as human corneal epithelial cells (HCECs). In this study, we evaluated the cytotoxicity induced by 50, 100 and 150 nm sizes of SiNPs on cultured HCECs for up to 48 hours. SiNPs were up-taken by HCECs inside cytoplasmic vacuoles. Cellular reactive oxygen species generation was mildly elevated, dose dependently, with SiNPs, but no significant decrease of cellular viability was observed up to concentrations of 100 μg/ml for three different sized SiNPs. Western blot assays revealed that both cellular autophagy and mammalian target of rapamycin (mTOR) pathways were activated with the addition of SiNPs. Our findings suggested that 50, 100 and 150 nm sized SiNPs did not induce significant cytotoxicity in cultured HCECs.

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Section: Discussionmentioning

confidence: 99%

The Effect of Silica Nanoparticles on Human Corneal Epithelial Cells

Park

Jeong

Hong

et al. 2016

Sci Rep

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“…An increase in Si levels was only found in the spleen of rats exposed to an oral dose of 2500 mg/kg bw/day (for 84 days) (van der Zande et al 2014), a dose level that exceeds the current recommendations in OECD guidelines by a factor of 2.5. If taken up by cells, SAS particles are usually located in vesicles and endocytic compartments, and also along actin fibres and nuclear invaginations (Tarantini et al 2015b); they are, however, never found in the nucleus. Reports to the contrary always refer to dye-labelled or otherwise modified silicon dioxide.…”

Section: Bioavailability After Oral Intakementioning

confidence: 99%

“…In vitro micronuclei tests were negative in human lymphocytes and BEAS2B cells, inconclusive in undifferentiated Caco-2 cells, and positive in some lung-derived cells at cytotoxic concentrations (NANOGENOTOX 2013; Tarantini et al 2015b; Tavares et al 2014; Zijno et al 2016). The findings of in vitro indicator tests were negative for pyrogenic and hydrated silica under non-cytotoxic conditions (cf.…”

Section: Genotoxicitymentioning

confidence: 99%

The safety of nanostructured synthetic amorphous silica (SAS) as a food additive (E 551)

Fruijtier-Pölloth¹

2016

Arch Toxicol

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Key messages Particle sizes of E 551 products are in the micrometre range. The typical external diameters of the constituent particles (aggregates) are greater than 100 nm. E 551 does not break down under acidic conditions such as in the stomach, but may release dissolved silica in environments with higher pH such as the intestinal tract. E 551 is one of the toxicologically most intensively studied substances and has not shown any relevant systemic or local toxicity after oral exposure. AbstractSynthetic amorphous silica (SAS) meeting the specifications for use as a food additive (E 551) is and has always been produced by the same two production methods: the thermal and the wet processes, resulting in E 551 products consisting of particles typically in the micrometre size range. The constituent particles (aggregates) are typically larger than 100 nm and do not contain discernible primary particles. Particle sizes above 100 nm are necessary for E 551 to fulfil its technical function as spacer between food particles, thus avoiding the caking of food particles. Based on an in-depth review of the available toxicological information and intake data, it is concluded that the SAS products specified for use as food additive E 551 do not cause adverse effects in oral repeated-dose studies including doses that exceed current OECD guideline recommendations. In particular, there is no evidence for liver toxicity after oral intake. No adverse effects have been found in oral fertility and developmental toxicity studies, nor are there any indications from in vivo studies for an immunotoxic or neurotoxic effect. SAS is neither mutagenic nor genotoxic in vivo. In intact cells, a direct interaction of unlabelled and unmodified SAS with DNA was never found. Differences in the magnitude of biological responses between pyrogenic and precipitated silica described in some in vitro studies with murine macrophages at exaggerated exposure levels seem to be related to interactions with cell culture proteins and cell membranes. The in vivo studies do not indicate that there is a toxicologically relevant difference between SAS products after oral exposure. It is noted that any silicon dioxide product not meeting established specifications, and/or produced to provide new functionality in food, requires its own specific safety and risk assessment.

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“…Biocompatibility assessment of silica coated nanoparticles performed in human vascular endothelial cells (HUVEC) and cancer cells derived from the cervix carcinoma (HeLa) showed cell type dependent nanotoxicity15. On the other hand, other studies reported no toxicity or dose-dependent effects in primary human cells16 as well as size-dependent effects17.…”

mentioning

confidence: 99%

In vitro biocompatibility study of sub-5 nm silica-coated magnetic iron oxide fluorescent nanoparticles for potential biomedical application

Foglia

Ledda

Fioretti

et al. 2017

Sci Rep

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Magnetic iron oxide nanoparticles (IONPs), for their intriguing properties, have attracted a great interest as they can be employed in many different biomedical applications. In this multidisciplinary study, we synthetized and characterized ultrafine 3 nm superparamagnetic water-dispersible nanoparticles. By a facile and inexpensive one-pot approach, nanoparticles were coated with a shell of silica and contemporarily functionalized with fluorescein isothiocyanate (FITC) dye. The obtained sub-5 nm silica-coated magnetic iron oxide fluorescent (sub-5 SIO-Fl) nanoparticles were assayed for cellular uptake, biocompatibility and cytotoxicity in a human colon cancer cellular model. By confocal microscopy analysis we demonstrated that nanoparticles as-synthesized are internalized and do not interfere with the CaCo-2 cell cytoskeletal organization nor with their cellular adhesion. We assessed that they do not exhibit cytotoxicity, providing evidence that they do not affect shape, proliferation, cellular viability, cell cycle distribution and progression. We further demonstrated at molecular level that these nanoparticles do not interfere with the expression of key differentiation markers and do not affect pro-inflammatory cytokines response in Caco-2 cells. Overall, these results showed the in vitro biocompatibility of the sub-5 SIO-Fl nanoparticles promising their safe employ for diagnostic and therapeutic biomedical applications.

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Toxicity, genotoxicity and proinflammatory effects of amorphous nanosilica in the human intestinal Caco-2 cell line

Cited by 83 publications

References 68 publications

The Effect of Silica Nanoparticles on Human Corneal Epithelial Cells

The Effect of Silica Nanoparticles on Human Corneal Epithelial Cells

The safety of nanostructured synthetic amorphous silica (SAS) as a food additive (E 551)

In vitro biocompatibility study of sub-5 nm silica-coated magnetic iron oxide fluorescent nanoparticles for potential biomedical application

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