Toxicity of clomazone and its formulations to zebrafish embryos (Danio rerio)

Stevanović, Marija; Gašić, Slavica; Pípal, Marek; Bláhová, Lucie; Brkic, Dragica; Nešković, Neško; Hilscherová, Klára

doi:10.1016/j.aquatox.2017.04.007

Cited by 28 publications

(18 citation statements)

References 46 publications

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“…The labeled analyte was not necessary to be separated after the antibody-analyte binging reaction. FPIA was applied to determine pesticides (Stevanovic et al, 2017) and mycotoxins (Zhang et al, 2017). It meets the requirement of simple, cost-effective, fast and high sample throughput.…”

Section: Introductionmentioning

confidence: 99%

Rapid and homologous immunoassay for the detection of herbicide propisochlor in water

Liu

Wang

Zhou

et al. 2017

Food and Agricultural Immunology

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The herbicide propisochlor can be introduced into the water cycle via agricultural activities, thus possibly leading to harmful ecological effects. It is significant for the development of a rapid method for propisochlor analysis in water. In this work, a rapid homogeneous immunoassay based on fluorescence polarization immunoassay (FPIA) was firstly developed for the determination of propisochlor in pond water. The developed FPIA assay can be completed within only 14 min; the 50% inhibition concentration (IC 50 ) was 125.1 ng/mL and the limit of detection was 8.4 ng/mL. The recoveries of propisochlor in pond water ranged from 80.0% to 86.6%. A high performance liquid chromatography confirmation showed a good correlation with the developed FPIA (R 2 = 0.9748). The developed FPIA exhibited satisfactory rapidity, high sensitivity and excellent specificity, meeting the standards of the screening assay of propisochlor in pond water on site. The molecular modeling showed that the alkoxyalkyl of chloroacetamide herbicides exerted important effects on the herbicide-antibody binding.

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Section: Introductionmentioning

confidence: 99%

Rapid and homologous immunoassay for the detection of herbicide propisochlor in water

Liu

Wang

Zhou

et al. 2017

Food and Agricultural Immunology

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“…Thus, careful choice of formulations is compulsory for environmental protection purposes. Moreover, the lethal effect of 2,4-D can be explained by an enhanced uptake of the active substance since the solvent can enhance its mobility in the aquatic ecosystem (Stevanovic et al 2017) or by a decrease in dissolved oxygen in water leading to asphyxiation (Pandey et al 2009). Death could have, therefore, occurred either by direct toxicity or indirectly by making the medium inauspicious for the fish development or even by both (Nwani et al 2010).…”

Section: Discussionmentioning

confidence: 99%

2,4-Dichlorophenoxyacetic acid herbicide effects on zebrafish larvae: development, neurotransmission and behavior as sensitive endpoints

Gaaied

Oliveira

Domingues

et al. 2019

Environ Sci Pollut Res

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Assessment of pesticides toxicity using zebrafish early life stages is relevant for aquatic systems safety. This study aimed to evaluate the short-term effects of 2,4-dichlorophenoxyacetic acid (2,4-D) on zebrafish (Danio rerio) embryos from 3 h post fertilization to 96 hpf. A set of 2,4-D concentrations ranging from 0.32 to 80 mg/L were tested and median lethal concentration (LC 50) at 96-h was calculated as 2.86 mg/L. A sub-teratogenic concentrations range from 0.02 to 0.8 mg/L was then used to assess effects at ontogenic, biochemical, and behavioral levels. The main developmental defects were tail deformities and pericardial edema at concentrations equal or above 0.32 mg/L. Cholinesterase activity (at 96 hpf) and larvae swimming behavior (at 120 hpf) were affected even at the lowest tested dose (0.02 mg/L). The behavior analysis was a sensitive endpoint, with a decrease in the swimming distance of exposed larvae during light period. The effect of 2,4-D in ChE was translated by an inhibition of the enzyme activity in all treated groups. These findings demonstrate that 2,4-D can alter the cholinergic system by affecting ChE activity which may be involved in the locomotion reduction of exposed larvae and emphasize the potential of neurotransmission and behavioral endpoints as early warning signs of herbicides contamination in aquatic ecosystems.

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“…Forms with the same active ingredient may differ in toxicity to both pests and non-target species. For instance, emulsifiable formulation is more toxic than aqueous capsule suspension [ 25 ]. Pesticide products from different manufacturers have different proportions of active ingredient and formulants.…”

Section: Commercial Formulations Of Pesticidesmentioning

confidence: 99%

“…One more problem is that the toxic effects of commercial pesticide formulations are equated to the effects of their active ingredients, which may result in incorrect assessment of their safety. Considerable research effort has been devoted recently to separation of the effects and detection of hidden hazards [ [22] , [23] , [24] , [25] , [26] , [27] , [28] , [29] , [30] ].…”

Section: Introductionmentioning

confidence: 99%

Pesticides: formulants, distribution pathways and effects on human health – a review

et al. 2021

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Toxicity of clomazone and its formulations to zebrafish embryos (Danio rerio)

Cited by 28 publications

References 46 publications

Rapid and homologous immunoassay for the detection of herbicide propisochlor in water

Rapid and homologous immunoassay for the detection of herbicide propisochlor in water

2,4-Dichlorophenoxyacetic acid herbicide effects on zebrafish larvae: development, neurotransmission and behavior as sensitive endpoints

Pesticides: formulants, distribution pathways and effects on human health – a review

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