Toxicity of nanoparticles embedded in paints compared to pristine nanoparticles, in vitro study

Smulders, Stijn; Luyts, Katrien; Brabants, Gert; Golanski, Luana; Martens, Johan A.; Vanoirbeek, Jeroen; Hoet, Peter

doi:10.1016/j.toxlet.2014.11.030

Cited by 29 publications

(18 citation statements)

References 29 publications

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“…There has been increasing concern regarding the toxicity of QDs, but further effort is needed to make them safe for biomedical application. 46 The toxic effects of different QDs have already been investigated in vitro 34,[47][48][49][50][51] as well as in vivo. 50,52 QDs are suggested to be cytotoxic and/or to change gene expression 53 and the cores and coatings of QDs may be responsible for their toxicity.…”

Section: Discussionmentioning

confidence: 99%

An <i>In Vitro</i> Study on the Cytotoxicity and Genotoxicity of Silver Sulfide Quantum Dots Coated with Meso-2,3-dimercaptosuccinic Acid

Vardar

Aydın

Hocaoglu

et al. 2019

tjps

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Gümüş sülfür (Ag 2 S) kuantum noktaları (QD), hem görüntüleme hem de ilaç/gen hedefleme için büyük aktiviteleri nedeniyle biyo-görüntüleme sisteminde oldukça gelecek vaad eden nanomalzemelerdir. Mezo-2,3-dimerkaptosüksinik asit (DMSA) ile kaplanmış Ag 2 S QD'lerin toksisitesi hakkında yeterli çalışma yoktur. Bu çalışmada Çin hamster akciğer fibroblast (V79) hücrelerinde DMSA ile kaplanmış Ag 2 S QD'lerin geniş bir konsantrasyon aralığında (5-2000 µg/mL) sitotoksisitesini belirlemeyi amaçladık. Gereç ve Yöntemler: Hücre canlılığı 3-(4,5-dimetiltiyazol-2-il)-2,5-difeniltetrazolium bromid (MTT) ve nötral kırmız alım (NRU) deneyleri ile belirlendi. DMSA/Ag 2 S QD'lerin genotoksik ve apoptotik etkileri sırasıyla komet analizi ve gerçek zamanlı polimeraz zincir reaksiyonu tekniği ile değerlendirildi. Bulgular: Ag 2 S QD'lerin en yüksek dozlarında hücre canlılığı MTT ve NRU deneylerinde sırasıyla 54.0±4.8% ve 65.7±4.1% olarak bulundu. Ancak hücre canlılığı 400 µg/mL (MTT deneyi) ve 800 µg/mL (NRU deney) üzerinde azalmıştır. İncelenen konsantrasyonlarda DNA hasarının DMSA/Ag 2 S QD'ler tarafından indüklenmediği belirlenmiştir. P53, kaspaz-3, kaspaz-9, Bax, Bcl-2 ve survivin genlerinin mRNA ekspresyon düzeyleri 500 ve 1000 µg/mL DMSA/Ag 2 S QD'lere maruz kalan hücrelerde değişmiştir. Sonuç: DMSA/Ag 2 S QD'lerin yüksek dozlarda sitotoksik etkilerinin apoptotik yollarla ortaya çıkabileceği görülmektedir. Bununla birlikte, DMSA/ Ag 2 S QD'ler, düşük dozlarda biyolojik olarak uyumlu görünmektedir, bu da onları hücre görüntüleme uygulamaları için uygun kılmaktadır.

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Section: Discussionmentioning

confidence: 99%

An <i>In Vitro</i> Study on the Cytotoxicity and Genotoxicity of Silver Sulfide Quantum Dots Coated with Meso-2,3-dimercaptosuccinic Acid

Vardar

Aydın

Hocaoglu

et al. 2019

tjps

View full text Add to dashboard Cite

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“…Stock suspensions (2 mg/ml) of powder samples (SWCNTs and MWCNTs) were prepared in pure H 2 O with 2% mouse serum by ultra-sonication (PTS Technics, Huddinge, Sweden) for 16 min as described [13]. NP stock suspensions were used within 1 h of preparation.…”

Section: Methodsmentioning

confidence: 99%

Changes in DNA Methylation in Mouse Lungs after a Single Intra-Tracheal Administration of Nanomaterials

Tabish¹,

Poels²,

Byun³

et al. 2017

PLoS ONE

Self Cite

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AimsThis study aimed to investigate the effects of nanomaterial (NM) exposure on DNA methylation.Methods and ResultsIntra-tracheal administration of NM: gold nanoparticles (AuNPs) of 5-, 60- and 250-nm diameter; single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs) at high dose of 2.5 mg/kg and low dose of 0.25 mg/kg for 48 h to BALB/c mice. Study showed deregulations in immune pathways in NM-induced toxicity in vivo. NM administration had the following DNA methylation effects: AuNP 60 nm induced CpG hypermethylation in Atm, Cdk and Gsr genes and hypomethylation in Gpx; Gsr and Trp53 showed changes in methylation between low- and high-dose AuNP, 60 and 250 nm respectively, and AuNP had size effects on methylation for Trp53.ConclusionEpigenetics may be implicated in NM-induced disease pathways.

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“…28, 30, 104 Results for textiles, paints, and nanocomposites 105 suggest that released particles significantly transform and age, and exhibit different environmental behavior and effects compared to pristine ENMs. 106, 107 Assessing changes in form and associated behavior or activity across the material's life cycle are uniquely challenging.…”

Section: What Exposure Conditions Are Used In Assessing Enm Ecologicamentioning

confidence: 99%

“…In addition, coatings, either on pristine ENMs or acquired in the test media or environment, may alter toxicity. 104, 183 …”

Section: What Exposure Conditions Are Used In Assessing Enm Ecologicamentioning

confidence: 99%

Considerations of Environmentally Relevant Test Conditions for Improved Evaluation of Ecological Hazards of Engineered Nanomaterials

Holden

Gardea‐Torresdey

Klaessig

et al. 2016

Environ. Sci. Technol.

199

150

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Engineered nanomaterials (ENMs) are increasingly entering the environment with uncertain consequences including potential ecological effects. Various research communities view differently whether ecotoxicological testing of ENMs should be conducted using environmentally relevant concentrations—where observing outcomes is difficult—versus higher ENM doses, where responses are observable. What exposure conditions are typically used in assessing ENM hazards to populations? What conditions are used to test ecosystem-scale hazards? What is known regarding actual ENMs in the environment, via measurements or modeling simulations? How should exposure conditions, ENM transformation, dose, and body burden be used in interpreting biological and computational findings for assessing risks? These questions were addressed in the context of this critical review. As a result, three main recommendations emerged. First, researchers should improve ecotoxicology of ENMs by choosing test endpoints, duration, and study conditions—including ENM test concentrations—that align with realistic exposure scenarios. Second, testing should proceed via tiers with iterative feedback that informs experiments at other levels of biological organization. Finally, environmental realism in ENM hazard assessments should involve greater coordination among ENM quantitative analysts, exposure modelers, and ecotoxicologists, across government, industry, and academia.

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Toxicity of nanoparticles embedded in paints compared to pristine nanoparticles, in vitro study

Cited by 29 publications

References 29 publications

An <i>In Vitro</i> Study on the Cytotoxicity and Genotoxicity of Silver Sulfide Quantum Dots Coated with Meso-2,3-dimercaptosuccinic Acid

An <i>In Vitro</i> Study on the Cytotoxicity and Genotoxicity of Silver Sulfide Quantum Dots Coated with Meso-2,3-dimercaptosuccinic Acid

Changes in DNA Methylation in Mouse Lungs after a Single Intra-Tracheal Administration of Nanomaterials

Considerations of Environmentally Relevant Test Conditions for Improved Evaluation of Ecological Hazards of Engineered Nanomaterials

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