Toxicity of TCDD in European flounder (Platichthys flesus) with emphasis on histopathology and cytochrome P450 1A induction in several organ systems

Grinwis, Guy C. M.; Besselink, H.; Brandhof, E.J. van den; Bulder, Astrid S.; Engelsma, Marc; Kuiper, Raoul V.; Wester, Piet W.; Vaal, Manon; Vethaak, A.D.; Vos, J.G.

doi:10.1016/s0166-445x(00)00084-9

Cited by 46 publications

(15 citation statements)

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“…10 d previously with 100 μg of TCDD/kg showed depressed plasma TT 4 levels but no changes in FT 4 or plasma TT 3 levels; there were no effects of higher or lower TCDD doses [44]. Oral administration of 0.0125 to 500 μg TCDD/kg over one to eight weeks did not alter thyroid histological appearance in the same species [45]. Some coplanar PCB congeners, such as 77 or 126, structurally resemble TCDDs and also could exert their actions through the ah receptor.…”

Section: Studies Of Thyroid Disruptionmentioning

confidence: 99%

Contaminant effects on the teleost fish thyroid

Brown

Adams

Cyr

et al. 2004

Enviro Toxic and Chemistry

227

139

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Abstract-Numerous environmentally relevant chemicals, including polychlorinated hydrocarbons, polycyclic aromatic hydrocarbons, organochlorine pesticides, chlorinated paraffins, organophosphorous pesticides, carbamate pesticides, cyanide compounds, methyl bromide, phenols, ammonia, metals, acid loads, sex steroids, and pharmaceuticals, exert acute or chronic effects on the thyroid cascade in the approximately 40 teleost fish species tested to date. Thyroid endpoints, therefore, serve as biomarkers of exposure to environmental pollutants. However, the mechanisms underlying thyroid changes and their physiological consequences are poorly understood because the thyroid cascade may respond indirectly and it has considerable capacity to compensate for abuses that otherwise would disrupt thyroid hormone homeostasis. Indeed, a xenobiotic-induced change in fish thyroid function has yet to be conclusively causally linked to decreased fitness or survival. Other complications in interpretation arise from the diversity of test conditions employed and the often indiscriminate use of numerous thyroid endpoints. Future work should be directed toward standardizing test conditions and thyroid endpoints and investigating causal links between thyroid changes and fish growth, reproduction, and development. Development may be particularly susceptible to thyroid disruption, and thyroid endpoints appropriate for early life stages need to be applied.

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Section: Studies Of Thyroid Disruptionmentioning

confidence: 99%

Contaminant effects on the teleost fish thyroid

Brown

Adams

Cyr

et al. 2004

Enviro Toxic and Chemistry

227

139

View full text Add to dashboard Cite

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“…Histological examination of thyroid follicles can provide an indication of disruption of thyroid hormone production by xenobiotic chemicals, and has been used to examine thyroid-disrupting effects of a number of different chemicals in fishes Dixon, 1994, 1996;Bhattacharya et al, 1995;Ram, 1998;Ricard et al, 1998;Grinwis et al, 2000;Cooley et al, 2001;Palace et al, 2001;Brown et al, 2004b;Park et al, 2004) and amphibians (Goleman et al, 2002a;Carr et al, 2003). Such toxicant-induced changes are based upon the physiology of the pituitary-thyroid axis.…”

Section: Introductionmentioning

confidence: 99%

Thyroid Endocrine Disruption in Stonerollers and Cricket Frogs from Perchlorate-Contaminated Streams in East-Central Texas

et al. 2005

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In October 2001 and March 2002, a field survey of central stonerollers (Campostoma anomalum) from perchlorate-contaminated streams in central Texas was conducted to assess thyroid endocrine disruption. A survey of adult male and female cricket frogs (Acris crepitans) was performed at the same site between 2001 and 2003. Perchlorate is an oxidizer primarily used in solid-fuel rockets, and many sites that processed or used perchlorate are now contaminated. Histological analysis revealed that the fish from contaminated sites had increased thyroid follicular hyperplasia, hypertrophy, and colloid depletion. Multivariate analysis was generally found to be more powerful than univariate analysis. Seasonal differences existed in the degree of thyroidal perturbation were discovered, and fish were generally less sensitive to thyroidal perturbations in March compared to October. Thyroidal histological indicators were also correlated to levels of perchlorate in the fish, water, and periphyton. Periphyton was frequently most strongly correlated to thyroidal indices, suggesting that exposure through the food chain may be of import. In addition, one of the presumed reference sites turned out to be contaminated with perchlorate, and this was reflected by thyroidal biomarkers before perchlorate was detected in the stream water or biota. There was no evidence of colloid depletion or hyperplasia in frogs from any of the sites, although frogs from two sites with greatest mean water perchlorate concentrations exhibited significantly greater follicle cell hypertrophy. Furthermore, there was a significant positive correlation between follicle cell height and mean water perchlorate concentrations for frogs collected from all sites. This is the first known published account of perchlorate-induced thyroid disruption in fish under field situations, only the second known published account for amphibians, and also points out the value of biomarkers for contaminant biomonitoring.

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“…Indeed, fish CYP1A induction by waterborne and ingested xenobiotic inducers has been previously demonstrated [27][28][29][30][31]. Protein and catalytic activity levels had simultaneously and significantly decreased within 144 d, presumably because of lower adverse environmental influence, but transcript level remained unchanged during the 318-d experimental period.…”

Section: Discussionmentioning

confidence: 83%

Quantitative evaluation of hepatic cytochrome P4501A transcript, protein, and catalytic activity in the striped sea bream (Lithognathus mormyrus)

Tom

Shmul

Shefer

et al. 2003

Enviro Toxic and Chemistry

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Hepatic cytochrome P4501A (CYP1A) expression was partially characterized in the striped sea bream (Lithognathus mormyrus) from the Mediterranean coast of Israel as part of the process of establishing the CYP1A gene as an environmental biomarker. Reverse transcription-competitive polymerase chain reaction, competitive enzyme-linked immunosorbent assay, and ethoxyresorufin O-deethylase (EROD) assay were used for evaluating transcript, protein, and catalytic activity levels, respectively, in absolute units. Highest elucidated transcript, protein, and catalytic activity levels were 0.264 +/- SD 0.084 fmol/microg total RNA, 0.88 +/- 0.52 pmol/microg total protein, and 1.11 +/- 0.52 pmol resorufin/min/microg total protein, respectively, and the lower levels were 0.009 +/- 0.007 fmol/microg total RNA, 0.17 +/- 0.08 pmol/microg total protein, and 0.11 +/- 0.06 pmol resorufin/min/microg total protein, respectively, demonstrating substantial induction potential. All alternate pairs of seven examined field samples, revealing a transcript-level ratio higher than 1.7, also demonstrated a significant difference between their transcript levels, indicating a potential to detect relatively small biomarker changes (1.7-fold) caused by environmental effects. Simultaneous triple measurements of transcript, protein, and catalytic activity were carried out in individuals from two field samples and during a 318-d decay experiment. Fish from the field samples revealed significant alternate bivariate correlation between transcript, protein, and enzymatic activity. Conflicting results were found when analyzing the decay experiment, in which both protein and catalytic activity levels decreased significantly to basal levels, in contrast to no significant change in transcript levels throughout the experiment. No significant difference was observed between males and females regarding the levels of CYP1A transcript, protein, and EROD.

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Toxicity of TCDD in European flounder (Platichthys flesus) with emphasis on histopathology and cytochrome P450 1A induction in several organ systems

Cited by 46 publications

References 50 publications

Contaminant effects on the teleost fish thyroid

Contaminant effects on the teleost fish thyroid

Thyroid Endocrine Disruption in Stonerollers and Cricket Frogs from Perchlorate-Contaminated Streams in East-Central Texas

Quantitative evaluation of hepatic cytochrome P4501A transcript, protein, and catalytic activity in the striped sea bream (Lithognathus mormyrus)

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