Toxicity of the aromatase inhibitor letrozole to Japanese medaka (Oryzias latipes) eggs, larvae and breeding adults

Sun, Liwei; Zha, Jinmiao; Spear, Philip A.; Wang, Zijian

doi:10.1016/j.cbpc.2007.01.017

Cited by 46 publications

(35 citation statements)

References 49 publications

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“…The adverse effects of E2 on males could not be ameliorated by coexposure with LET, which was substantially reflected in the reproductive performance. In our previous study (Sun et al, 2007b), we showed that exposure to LET alone reduced plasma VTG levels in females in a dose-dependent manner. Moreover, dose-dependent decreases in fecundity and fertility accompanied by histological changes in females (inhibition of oocyte growth and possibly maturation) were observed.…”

Section: Discussionmentioning

confidence: 76%

“…Moreover, dose-dependent decreases in fecundity and fertility accompanied by histological changes in females (inhibition of oocyte growth and possibly maturation) were observed. For males, no significant changes were found except for changes in histology in fish exposed to high levels of LET (Sun et al, 2007b).…”

Section: Discussionmentioning

confidence: 78%

“…The samples were analyzed using a 6890 gas chromatograph/5973 mass spectrometer (Agilent, USA). For measurement of LET, the analytical method was performed as described by Pfister et al (1994) with some modifications (Sun et al, 2007b). The water samples were liquid/liquid extracted using dichloromethane.…”

Section: Analytical Chemistrymentioning

confidence: 99%

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Effects of binary mixtures of estrogen and antiestrogens on Japanese medaka (Oryzias latipes)

2009

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a b s t r a c tIt is supposed that the estrogenic effects of estrogenic chemicals will be balanced by the presence of other endocrine-disrupting chemicals (EDCs) with different properties. However, little convincing research into this issue has been carried out to date. In the present study, the combined effects of EDCs with estrogenic and antiestrogenic activities were assessed using a 21-day reproduction assay with the Japanese medaka (Oryzias latipes) as an in vivo model. Natural estrogen (17␤-estradiol, E2) and the pharmaceutical substances letrozole and tamoxifen, which are both model antiestrogens, were tested as reference compounds individually and in combination. Exposure to E2 (200 ng/L) alone resulted in significant biological changes in paired fish, including impaired reproductive capacity and plasma vitellogenin induction. Upon coexposure with tamoxifen or letrozole (10, 50 and 250 g/L), the effects of estrogen on some biomarkers (e.g., plasma vitellogenin concentration) could be neutralized to some extent, but the impairments in reproductive performance were hardly ameliorated or even became more severe. Since reproductive performance is a more holistic parameter with population-level relevance, the possibility that the effects of estrogenic chemicals can be cancelled out by the presence of antiestrogens in aquatic organisms should be questioned. Our observations also illustrate the importance of considering multiple endpoints at different biological levels of organisms for accurate and adequate assessment of the integrated effects of EDCs, especially in environmentally relevant exposure situations.

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Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 78%

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Effects of binary mixtures of estrogen and antiestrogens on Japanese medaka (Oryzias latipes)

2009

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“…Fertilized eggs of Japanese medaka were exposed to tamoxifen for 14 days. Adverse effects on hatchability and the time required to hatch were only found at a concentration of at least 125 µg/L [79,80]. After released into the wastewater, tamoxifen is able to undergo photolysis after prolonged exposure to sunlight radiation.…”

Section: Cytotoxic Antibiotics (Anthracycline): Doxorubicin Epirubicmentioning

confidence: 99%

Occurrence, Ecotoxicology, and Treatment of Anticancer Agents as Water Contaminants

Xie¹

2012

J Environ Anal Toxicol

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“…Recently, the authors of this work have also described a method to determine LE and its metabolite in human urine by MEKC [25]. Sun et al [26] have studied the toxicity of the LE to Japanese medaka eggs, larvae and breeding adults using the method described by Marfil et al with some modifications.…”

Section: Introductionmentioning

confidence: 99%

Rapid quantitative analysis of letrozole, fluoxetine and their metabolites in biological and environmental samples by MEKC

2009

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A micellar electrokinetic chromatographic method has been developed to analyze biological (human serum, saliva and urine) and environmental samples (three different water samples) for letrozole (LE), fluoxetine and their main metabolites. For this purpose a 20 mM borate buffer (pH 9.5) containing 20 mM SDS and 12% v:v 2-propanol was used as the background electrolyte. The samples were hydrodynamically injected for 6 s, separated in a fused-silica capillary at 25 kV and 50 degrees C and detected at 230 nm. Under these conditions, the migration times for all the studied compounds ranged from 3.0 up to 8.0 min. Linearity ranges were determined as 125-1500 ng/mL, whereas detection limits were from 37 to 120 ng/mL in biological samples and a value of 6 ng/mL in water samples. According to the validation study, the developed method was proved to be accurate, precise, sensitive, specific, rugged and robust. This method was applied to the analysis of different biological fluids at clinical levels, including two urine samples from patients undergoing treatment with LE or fluoxetine, and also to environmental samples at potentially polluting level. Prior to the determination, the samples were purified and pre-concentrated by means of an extraction-preconcentration step with a C18 cartridge and by eluting the compounds with methanol.

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Toxicity of the aromatase inhibitor letrozole to Japanese medaka (Oryzias latipes) eggs, larvae and breeding adults

Cited by 46 publications

References 49 publications

Effects of binary mixtures of estrogen and antiestrogens on Japanese medaka (Oryzias latipes)

Effects of binary mixtures of estrogen and antiestrogens on Japanese medaka (Oryzias latipes)

Occurrence, Ecotoxicology, and Treatment of Anticancer Agents as Water Contaminants

Rapid quantitative analysis of letrozole, fluoxetine and their metabolites in biological and environmental samples by MEKC

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