Toxicity profiling and antioxidant activity of ethyl acetate extract of leaves of Premna integrifolia L. for its application as protective agent against xenobiotics

Singh, Chandrashekhar; Tiwari, Kavindra Nath; Kumar, Pradeep; Kumar, Anil; Dixit, Jyoti; Saini, Rajesh; Mishra, Sunil Kumar

doi:10.1016/j.toxrep.2021.01.004

Cited by 15 publications

(7 citation statements)

References 42 publications

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“…Besides that, the in vitro anti-lipid peroxidation capability of EDME also showed similar potential compared to E. hyemale (Pandey & Khatoon, 2017). The anti-lipid peroxidation and antioxidant properties of EDME indicated its role in the medication of several in ammatory diseases, as anti-oxidants are reported to protect tissue from damage and prevents unwanted in ammatory responses (C. Singh et al, 2021).…”

Section: Discussionmentioning

confidence: 83%

Exploring the ameliorative role of Equisetum diffusum D. Don whole-plant methanolic-extract in acute inflammation and molecular docking analysis of GC-MS-identified phytocompounds with few prominent inflammatory markers/cytokines for inspecting the potent drug targets.

Sarkar

Modak

Haydar

et al. 2023

Preprint

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Equisetum diffusum D. Don (Fam. Equisetaceae), commonly known as ‘Himalayan horsetail’ is an important medicinal pteridophyte species having various ethnobotanical properties; and traditionally been used in the treatment of back pain, bone fracture, bone dislocation, and arthritis by various tribal communities of India. Although the pharmacological uses are known, no study related to its ethnobotanical validation in experimental animal models has been reported till date. The goal of the present study was to validate the anti-inflammatory properties of the whole plant methanolic-extract (EDME) using in silico, in vitro,and in vivo strategies. The GC-MS analysis of EDME detected the presence of 47 phyto-compounds, out of which Stigmasta-3,5-dien-7-one and 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl showed good inhibition of cyclooxygenase-2 (Cox-2) and IL-6 respectively compared to standard NSAIDs, in docking analysis. In vitro anti-inflammatory studies indicated that EDME inhibits 48.2 ± 3.74% of protein denaturation at a concentration of 1000 μg/ml compared to standard diclofenac sodium (57.6 ± 3.70%at 100 μg/ml concentration). The similar concentration (1000 μg/ml) of extract inhibits heat-induced (33.6 ± 2.55%) and hypotonicity-induced (58.1 ± 3.39%) erythrocyte membrane lyses respectively, compared to indomethacin standard (45.6 ± 2.36% and 67.2 ± 1.45% respectively at 200 μg/ml concentration). The in vivoanti-inflammatory study of EDME showed 52.26% and 73.36% reduction in paw-edema in both low and high-dose protective groups respectively, when compared to the carrageenan control-group. Our findings established the anti-inflammatory roles of the whole plant methanolic extract of Equisetum diffusum on strong ground which may encourage drug-development for the treatment of inflammation-related complications.

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Section: Discussionmentioning

confidence: 83%

Exploring the ameliorative role of Equisetum diffusum D. Don whole-plant methanolic-extract in acute inflammation and molecular docking analysis of GC-MS-identified phytocompounds with few prominent inflammatory markers/cytokines for inspecting the potent drug targets.

Sarkar

Modak

Haydar

et al. 2023

Preprint

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“…Toxicological parameters are very essential to study which ensure the safe dose of plant-based natural products (Singh et al, 2021). The study of hematological parameters on female Balb/C mice suggested that there was no hematological toxicity induced within RTME-administrated mice groups and there was no significant weight loss in experimental mice groups till 40 days.…”

Section: Discussionmentioning

confidence: 99%

Crude extract ofRuellia tuberosaL. flower induces intracellular ROS, promotes DNA damage and apoptosis in Triple Negative Breast Cancer Cells

Guha,

Talukdar,

Mandal

et al. 2024

Preprint

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Ethnophamacological relevance: In the traditional folklore medicine system, the primary uses of Ruellia tuberosa L. include as a diuretic, anti-hypertensive, antipyretic, anti-diabetic, analgesic, and gastroprotective agent. Some reports also demonstrated that it has been used to treat gonorrhea-like diseases. Purpose: Exploring the anti-cancer potential of the methanolic extract of Ruellia tuberosa L. flower (RTME) with special emphasis on human triple-negative breast cancer (TNBC) and investigating the possible signaling networks and regulatory pathways underlying it. Methods: Preparation of RTME and identifying the possible phytochemicals through GC-MS analysis. The anti-cancer potential of RTME was executed through in-vitro cytotoxicity assay, clonogenic assay, wound healing assay, ROS generation assay, cell cycle arrest, apoptotic nuclear morphology study, cellular apoptosis study, mitochondrial membrane potential (MMP) alteration study, protein and gene expressions alteration study. Apart from this, toxicological status and in-silico molecular docking studies were also conducted. Results: From this study, it was obvious that several phytochemicals within RTME have the potential to act as anti-cancerous agents. RTME was found to exhibit significant in-vitro cytotoxicity along with a reduction in colony formation and inhibition of cell migratory potential in MDA-MB-231 cells. RTME also induced intracellular ROS, promoted G0/G1 cell cycle arrest, caused mitochondrial membrane potential (MMP) alteration, and promoted cell death. From the pro- and anti-apoptotic marker study through the western blot and the qRT-PCR analysis, it was revealed that RTME promoted the intrinsic pathway of apoptosis. Furthermore, blood parameters and histological analysis revealed that RTME does not exhibit any toxic effect on female Balb/C mice. Finally, an in-silico molecular docking study revealed that the three identified lead phytochemicals in RTME show strong receptor-ligand interactions with the anti-apoptotic Bcl-2 and give a clue to the possible molecular mechanism of the RTME extract. Conclusion: From the findings, it was concluded that RTME has a significant therapeutic potential against TNBC which could be an alternative option for anti-cancer drug development.

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“…This study used two dose regimes of 500 mg/kg and 1000 mg/kg. The choice of these doses stemmed from an LD50 study we conducted according to the method of Lodhi et al [42] and Singh et al [43], which showed that ethanol extract of A.cordifolia leaves was not toxic since LD50 was greater than 5000 mg/kg body weight. All pharmacological treatments were administered orally.…”

Section: Experimental Designmentioning

confidence: 99%

Ameliorative potentials of ethanol extract of Alchornea cordifolia on some biochemical and physiological parameters in high-fat diet-induced obese Wistar rats

Ekeleme,

Odey,

Ibeneme

et al. 2024

Preprint

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This study investigated the effect of ethanol extract of A. cordifolia leaves on high-fat diet (HFD)-induced obesity and its associated metabolic abnormalities in rats. Thirty male rats were randomly divided into five groups (Six rats each)- Normal control, Obese control, Orlistat group, A.cordifolia groups (500 and 1000 mg/kg body weight). Dietary intake, anthropometric indices, lipid profile, atherogenic indices, blood glucose, hepatic and cardiac function, HMG CoA reductase activity and antioxidative properties were investigated. Intake of HFD led to significant increases in bodyweight, BMI, Lee's index, waist-circumference, liver, adipose tissue, heart weight and lipids, T.cholesterol, triglycerides, atherogenic index, and LDL-c, while decreasing HDL-c. Additionally, HFD consumption increased fasting and serum blood glucose, insulin, HOMA-IR, amylase, and lipase activity, while SOD, GPx, and GSH levels were reduced, and MDA increased. Moreover, HFD increased ALT, ALP, AST, LDH and creatine kinase levels. Furthermore, HMG-CoA reductase activity decreased, and histological analysis revealed inflammation, fat accumulation, and distortion in adipose tissue architecture in the liver and adipose tissue of obese rats. A.cordifolia supplementation effectively reversed HFD-induced alterations, demonstrating weight-reducing, anti-hyperlipidemic, antioxidant, and anti-inflammatory properties. Therefore, A.cordifolia possesses anti-obesity potential and may be used as a possible therapeutic alternative for obesity and its associated pathologies.

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Toxicity profiling and antioxidant activity of ethyl acetate extract of leaves of Premna integrifolia L. for its application as protective agent against xenobiotics

Cited by 15 publications

References 42 publications

Exploring the ameliorative role of Equisetum diffusum D. Don whole-plant methanolic-extract in acute inflammation and molecular docking analysis of GC-MS-identified phytocompounds with few prominent inflammatory markers/cytokines for inspecting the potent drug targets.

Exploring the ameliorative role of Equisetum diffusum D. Don whole-plant methanolic-extract in acute inflammation and molecular docking analysis of GC-MS-identified phytocompounds with few prominent inflammatory markers/cytokines for inspecting the potent drug targets.

Crude extract ofRuellia tuberosaL. flower induces intracellular ROS, promotes DNA damage and apoptosis in Triple Negative Breast Cancer Cells

Ameliorative potentials of ethanol extract of Alchornea cordifolia on some biochemical and physiological parameters in high-fat diet-induced obese Wistar rats

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