Toxicological Studies on Certain Medical Grade Plastics Sterilized by Ethylene Oxide

O'Leary, R.K.; Guess, Wallace L.

doi:10.1002/jps.2600570102

Cited by 62 publications

(19 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For each sample, its hemolytic potential was tested according to the method reported by O'Leary and Guess [37]. Human blood (0.1 mL) anticoagulated with citrate was added to 5 mL of PBS containing the samples with different amounts in test tubes.…”

Section: Methodsmentioning

confidence: 99%

Folate-targeted star-shaped cationic copolymer co-delivering docetaxel and MMP-9 siRNA for nasopharyngeal carcinoma therapy

Liu

Wang

et al. 2016

Oncotarget

View full text Add to dashboard Cite

The co-delivery of drug and gene has become the primary strategy in cancer therapy. Based on our previous work, to co-deliver docetaxel (DOC) and MMP-9 siRNA more efficiently for HNE-1 nasopharyngeal carcinoma therapy, a folate-modified star-shaped copolymer (FA-CD-PLLD) consisting of β-cyclodextrin (CD) and poly(L-lysine) dendron (PLLD) was synthesized, and then used for DOC and MMP-9 co-delivery. Different from commonly used amphiphilic copolymers micelles, the obtained CD derivative could be used directly for the combinatorial delivery of nucleic acid and hydrophobic DOC without a complicated micellization process. In vitro and in vivo assays are carried out to confirm the effectiveness of the target strategy and combined treatment. It was found that the conjugation of CD-PLLD with FA could enhance the DOC/MMP-9 delivery effect obviously, inducing a more significant apoptosis and decreasing invasive capacity of HEN-1 cells. In vivo assays showed that FA-CD-PLLD/DOC/MMP-9 could inhibit HNE-1 tumor growth and decrease PCNA expression effectively, indicating a promising strategy for nasopharyngeal carcinoma therapy. Moreover, the in vivo distribution of DOC and MMP-9, blood compatibility and toxicity are also explored.

show abstract

Section: Methodsmentioning

confidence: 99%

Folate-targeted star-shaped cationic copolymer co-delivering docetaxel and MMP-9 siRNA for nasopharyngeal carcinoma therapy

Liu

Wang

et al. 2016

Oncotarget

View full text Add to dashboard Cite

show abstract

“…30 Cytotoxicity assay was performed with the polyurethane ureas (0.5 × 0.5cm) by direct contact of the polymers with a monolayer of mouse fibroblast cells (L929) per ASTM standard F 813-83. The morphology of the cells was assessed by a phase contrast inverted microscope (Leitz DMIL) in comparison with negative control (cells on culture plate without material) and positive control (zinc mercapto benzothiol with the cells)…”

Section: Evaluation Of In Vitro Biocompatibilitymentioning

confidence: 99%

“…The percentage of hemolysis was found to be 0.48 for HFL9-PU1, 1.28 for HFL13-PU2, and 0.13 for HFL16-PU3, well within the permissible limit (5%). 30 These polymers can hence be classified as nonhemolytic.…”

Section: Synthesis and Characterizationmentioning

confidence: 99%

Studies on the effect of virtual crosslinking on the hydrolytic stability of novel aliphatic polyurethane ureas for blood contact applications

Thomas

Jayabalan

2001

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

The effect of virtual crosslinking on the hydrolytic stability of completely aliphatic novel poly(urethane ureas), HFL9-PU1 (hard-segment content 57.5%) and HFL13-PU2 (hard-segment content 67.9%) based on 4,4'-methylene bis(cyclohexyl isocyanate) (H(12)MDI)-hydroxy-terminated polybutadiene-1,6-hexamethylene diamine, was studied. Fourier transform infrared-attenuated total reflectance and wide-angle X-ray diffraction studies revealed hydrogen-bonding interaction and microphase separation and formation of crystallites by short- and long-range ordering in hard-segment domains. Three-dimensional networks from hydrogen bonding in the present polymers lead to virtually crosslinking and insolubility. These polymers were noncytotoxic to L929 fibroblast cells. The hemolytic potential is below the accepted limit. The studies on in vitro biostability in Ringer's solution, phosphate buffered saline, and papain enzyme revealed no weight loss. The infrared spectral studies revealed changes in the surface, especially on HFL9-PU1 aged in Ringer's solution and phosphate buffered saline, and no changes when aged in papain. The marginal changes noticed in tensile properties were attributed to the changes in degree of hydrogen bonding and associated rearrangement of molecular structure in the bulk. The results revealed that the lesser the crosslinking in virgin polymer, the higher the crosslinking in aged polymer and vice versa. Increased crosslinking during aging provided increased tensile properties in the aged polymer over the virgin polymer and vice versa. For comparison, an aliphatic polyetherurethane urea (HFL16-PU3) was also synthesized using poly(oxy tetra methylene glycol) in addition to the above reactants. Though both HFL9-PU1 and HFL16-PU3 contained the same hard-segment content, the aged sample of the latter showed decreased tensile properties with increased crosslinking during aging in contrast to the former. This was attributed to less microphase separation in the virgin HFL16-PU3 polymer.

show abstract

“…Ethylene oxide treatment is understood to deposit a toxic residue on surfaces that remains even after a 6 to 12 h forced draft aeration period (5). For this reason we flush the Nitex screen and other critical surfaces with a generous volume of culture medium prior to use.…”

Section: Discussionmentioning

confidence: 99%

Nylon-screen filtration devices for crude separation of dissociated cells for primary culture

McAteer

Cavanagh

1983

Journal of Tissue Culture Methods

View full text Add to dashboard Cite

show abstract

Toxicological Studies on Certain Medical Grade Plastics Sterilized by Ethylene Oxide

Cited by 62 publications

References 10 publications

Folate-targeted star-shaped cationic copolymer co-delivering docetaxel and MMP-9 siRNA for nasopharyngeal carcinoma therapy

Folate-targeted star-shaped cationic copolymer co-delivering docetaxel and MMP-9 siRNA for nasopharyngeal carcinoma therapy

Studies on the effect of virtual crosslinking on the hydrolytic stability of novel aliphatic polyurethane ureas for blood contact applications

Nylon-screen filtration devices for crude separation of dissociated cells for primary culture

Contact Info

Product

Resources

About