Toxin-antitoxin HicAB regulates the formation of persister cells responsible for the acid stress resistance in Acetobacter pasteurianus

Xia, Kai; Han, Chengcheng; Xu, Jun; Liang, Xinmiao

doi:10.1007/s00253-020-11078-w

Cited by 17 publications

(19 citation statements)

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“…However, only a few TAs on prophages have been identified (Jurenas et al, 2022 ). Previously, we have investigated the chromosomal hicB-hicA in A. pasteurianus Ab3 and annotated its functions (Xia et al, 2019 , 2021a , b ). The hicB-hicA was found on the phage phiS10 of Streptococcus suis and suggested a mechanism for the maintenance of the temperate phage (Tang et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Comprehensive deciphering prophages in genus Acetobacter on the ecology, genomic features, toxin–antitoxin system, and linkage with CRISPR-Cas system

Qian

Liang

et al. 2022

Front. Microbiol.

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Acetobacter is the predominant microbe in vinegar production, particularly in those natural fermentations that are achieved by complex microbial communities. Co-evolution of prophages with Acetobacter, including integration, release, and dissemination, heavily affects the genome stability and production performance of industrial strains. However, little has been discussed yet about prophages in Acetobacter. Here, prophage prediction analysis using 148 available genomes from 34 Acetobacter species was carried out. In addition, the type II toxin–antitoxin systems (TAs) and CRISPR-Cas systems encoded by prophages or the chromosome were analyzed. Totally, 12,000 prophage fragments were found, of which 350 putatively active prophages were identified in 86.5% of the selected genomes. Most of the active prophages (83.4%) belonged to the order Caudovirales dominated by the families Siphoviridae and Myroviridae prophages (71.4%). Notably, Acetobacter strains survived in complex environments that frequently carried multiple prophages compared with that in restricted habits. Acetobacter prophages showed high genome diversity and horizontal gene transfer across different bacterial species by genomic feature characterization, average nucleotide identity (ANI), and gene structure visualization analyses. About 31.14% of prophages carry type II TAS, suggesting its important role in addiction, bacterial defense, and growth-associated bioprocesses to prophages and hosts. Intriguingly, the genes coding for Cse1, Cse2, Cse3, Cse4, and Cas5e involved in type I-E and Csy4 involved in type I-F CRISPR arrays were firstly found in two prophages. Type II-C CRISPR-Cas system existed only in Acetobacter aceti, while the other Acetobacter species harbored the intact or eroded type I CRISPR-Cas systems. Totally, the results of this study provide fundamental clues for future studies on the role of prophages in the cell physiology and environmental behavior of Acetobacter.

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Section: Discussionmentioning

confidence: 99%

Comprehensive deciphering prophages in genus Acetobacter on the ecology, genomic features, toxin–antitoxin system, and linkage with CRISPR-Cas system

Qian

Liang

et al. 2022

Front. Microbiol.

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“…The HigB enhanced the tolerance to bile salt in W. cibaria 018 and E. coli BL21 Persistence in bacteria is a dormant physiological state, and it can evade various environmental stress by dropped metabolic activity. 14,15 In HigBA, the HigA protein can be selectively degraded by cellular proteases such as Lon and ClpXP, 49 then HigB is released, and results in the persister cells formation. 50 Thus, the HigAs in HigB-(HigA) 2 -HigB were selectively degraded when the W. cibaria 018 was exposed to bile salt stress, followed by forming the persistence.…”

Section: Analysis Of Higba Structurementioning

confidence: 99%

“…TA systems are important mediators of persister cell formation. 15,16 However, it was found that the persister cells of E. coil were not formed when the TA expressed, but escaped from phage infection. 17 Currently, HigBA could induce the formation of persister cells of W. cibaria 018 under bile salt stress, and their cell morphology varied with the bile salt concentration.…”

Section: Analysis Of Higba Structurementioning

confidence: 99%

“…TA systems are widely distributed in bacterial genome, and they are small operons involved in stress response management, which regulate persister cell formation to escape the various adverse environments. [14][15][16] At present, the type I-VIII of TA systems have been identified and classified by the composition and properties of antitoxin. 17 Among TA systems, the type II is the largest and best-studied class, and it is composed of two genes encoding a stable toxin protein that inhibits cell growth by interfering with vital processes and its cognate labile antitoxin protein that protects the cell from the toxin.…”

Section: Introductionmentioning

confidence: 99%

“…16 In normal cells, the antitoxin proteins usually conjunct to its cognate toxin to form a tight TA complex, and the complex inhibits the transcription of the type II TA operon by binding to the operator site. When bacteria expose to adverse environment, the antitoxin protein is hydrolyzed and TA complex is liberated from the operator site, and toxin protein is released, then the persister cells are formed to escape from the environmental stress, including antibiotics, 14 acid 15 and nutrition starvation. 18 The W. cibaria 018 (CGMCC 1.19376) has ten type II TA system pairs (Supporting Information, Fig.…”

Section: Introductionmentioning

confidence: 99%

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HigBA toxin–antitoxin system of Weissella cibaria is involved in response to the bile salt stress

et al. 2022

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BACKGROUND: Toxin-antitoxin (TA) systems are prevalent adaptive genetic elements in bacterial genomes, which can respond to environmental stress. While, few studies have addressed TA systems in probiotics and their roles in the adaptation to gastrointestinal transit (GIT) environments. RESULTS:The Weissella cibaria 018 could survive in pH 3.0-5.0 and 0.5-3.0 g L −1 bile salt, and its HigBA system responded to the bile salt stress, but not to acid stress. The toxin protein HigB and its cognate antitoxin protein HigA had 85.1% and 100% similarity with those of Lactobacillus plantarum, respectively, and they formed the stable tetramer HigB-(HigA) 2 -HigB structure in W. cibaria 018. When exposed to 1.5-3.0 g L −1 bile salt, the transcriptions of higB and higA were up-regulated with 4.39-19.29 and 5.94-30.91 folds, respectively. Meanwhile, W. cibaria 018 gathered into a mass with 48.07% survival rate and its persister cells were found to increase 8.21% under 3.0 g L −1 bile salt.CONCLUSION: The HigBA TA system of W. cibaria 018 responded to the bile salt stress, but not to acid stress, which might offer novel perspectives to understand the tolerant mechanism of probiotics to GIT environment.

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Inhibition of hyphal formation together with biochar addition promotes erythritol production by Yarrowia lipolytica

Liu,

Xia,

Chen

et al. 2024

Biotech & Bioengineering

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This study aimed to investigate the effect of hyphal formation in Yarrowia lipolytica and biochar addition on erythritol production by submerged fermentation. Hyphal formation significantly inhibited erythritol production by Y. lipolytica. Transcriptome analysis suggested that the impaired erythritol synthesis of hyphal cells was associated with the differential expression of genes involved in amino acid metabolism, lipid metabolism, and cell wall stability. Deletion of RAS2 responsible for yeast‐to‐hypha transition and EYD1 included in erythritol degradation blocked hyphal formation and improved erythritol production. Biochar prepared from corncob, sugarcane bagasse (SB), corn straw, peanut shell, coconut shell, and walnut shell (WS) had a positive effect on erythritol production, of which WS pyrolyzed at 500°C (WSc) performed the best in flask fermentation. In a 3.7 L bioreactor, 220.20 ± 10 g/L erythritol with a productivity of 2.30 ± 0.10 g/L/h was obtained in the presence of 1.4% (w/v) WSc and 0.7% SBc (SB pyrolyzed at 500°C) within 96 h. These results suggest that inhibition of hyphal formation together with biochar addition is an efficient way to promote erythritol production.

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Toxin-antitoxin HicAB regulates the formation of persister cells responsible for the acid stress resistance in Acetobacter pasteurianus

Cited by 17 publications

References 50 publications

Comprehensive deciphering prophages in genus Acetobacter on the ecology, genomic features, toxin–antitoxin system, and linkage with CRISPR-Cas system

Comprehensive deciphering prophages in genus Acetobacter on the ecology, genomic features, toxin–antitoxin system, and linkage with CRISPR-Cas system

HigBA toxin–antitoxin system of Weissella cibaria is involved in response to the bile salt stress

Inhibition of hyphal formation together with biochar addition promotes erythritol production by Yarrowia lipolytica

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