Tpx-1 is a component of the outer dense fibers and acrosome of rat spermatozoa

O'Bryan, Moira K; Sebire, Kimberley L; Meinhardt, Andreas; Edgar, Kimberley; Keah, Hooi-Hong; Hearn, Milton Thomas William; Kretser, David M de

doi:10.1002/1098-2795(200101)58:1<116::aid-mrd14>3.0.co;2-8

Cited by 83 publications

(84 citation statements)

References 37 publications

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“…Consistent with previous publications on mouse and human CRISP2 localization (Maeda et al, 1999;O'Bryan et al, 2001;Jamsai et al, 2008b), staining was observed in round through to elongated spermatids where the protein appeared to be incorporated into the growing sperm tail and the acrosome on the apical aspect of the sperm head (Fig. 4A).…”

Section: Crisp Protein Localizationsupporting

confidence: 91%

“…The respective tissues were harvested from mice and fixed in Bouin's fixative. Tissue were processed into paraffin and sectioned as described previously (O'Bryan et al, 2001). Following dewaxing and rehydration, sections underwent a period of antigen retrieval in Citrate Buffer (10 mM tri-sodium citrate, pH 6.0), followed by two 5-min washes in phosphate buffered saline (PBS) (10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , 2.7 mM KCl, 137 mM NaCl, pH 7.4).…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…Crisp2 is not regulated by androgens and its mRNA is first expressed in pachytene spermatocytes (Haendler et al, 1997;O'Bryan et al, 1998). Following a period of translational delay, CRISP2 protein is incorporated into the developing sperm acrosome and the sperm connecting piece and tail (Hardy et al, 1988;Foster and Gerton, 1996;O'Bryan et al, 2001). As indicated above, CRISP2 can regulate RyR activity .…”

Section: Introductionmentioning

confidence: 99%

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Cysteine‐rich secretory proteins are not exclusively expressed in the male reproductive tract

Reddy¹,

Gibbs²,

Merriner³

et al. 2008

Developmental Dynamics

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The Cysteine-RIch Secretory Proteins (CRISPs) are abundantly produced in the male reproductive tract of mammals and within the venom of reptiles and have been shown to regulate ion channel activity. CRISPs, along with the Antigen-5 proteins and the Pathogenesis related-1 (Pr-1) proteins, form the CAP superfamily of proteins. Analyses of EST expression databases are increasingly suggesting that mammalian CRISPs are expressed more widely than in the reproductive tract. We, therefore, conducted a reverse transcription PCR expression profile and immunohistochemical analyses of 16 mouse tissues to define the sites of production of each of the four murine CRISPs. These data showed that each of the CRISPs have distinct and sometimes overlapping expression profiles, typically associated with the male and female reproductive tract, the secretory epithelia of exocrine glands, and immune tissues including the spleen and thymus. These investigations raise the potential for a role for CRISPs in general mammalian physiology. Developmental Dynamics 237:3313-3323, 2008.

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Section: Crisp Protein Localizationsupporting

confidence: 91%

Section: Immunohistochemistrymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cysteine‐rich secretory proteins are not exclusively expressed in the male reproductive tract

Reddy¹,

Gibbs²,

Merriner³

et al. 2008

Developmental Dynamics

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“…To confirm the validity of this observation, testis homogenates and isolated caudal epididymal sperm and sperm tails (O'Bryan et al, 2001) were solublized in RIPA buffer and 2ϫ SDS-PAGE buffer, respectively, and run into 8% SDS-PAGE gels using standard methods. Following transfer to PDF membrane, the presence or absence of FGFR-1 was determined using a FGFR-1 specific antiserum at a concentration of 4 g/ml.…”

Section: The Identification Of Fgfr Signaling Components In Sperm Devmentioning

confidence: 94%

“…Transgene-derived protein within testis sections was detected by immunohistochemistry using a flag-tag monoclonal antibody at a concentration of 10 g/ml using the DAKO EnVision+ System (DakoCytomation, Glostrup, Denmark) as outlined by the manufacturer, following antigen retrieval as described ealier (O'Bryan et al, 2001). Specificity was assured by the absence of staining in wild-type testes processed in parallel.…”

Section: Transgene Expression Analysismentioning

confidence: 99%

FGFR-1 signaling is involved in spermiogenesis and sperm capacitation

Cotton

Gibbs

Sanchez-Partida

et al. 2006

Journal of Cell Science

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Cloning of the fibroblast growth factor receptor (FGFR) adaptor Snt-2 cDNA and the identification of FGFR-1 protein in association with sperm tails, suggested that FGFR-1 signaling was involved in either sperm tail development or function. This hypothesis was tested by the creation of transgenic mice that specifically expressed a dominant-negative variant of FGFR-1 in male haploid germ cells. Mating of transgenic mice showed a significant reduction in pups per litter compared with wild-type littermates. Further analysis demonstrated that this subfertility was driven by a combination of reduced daily sperm output and a severely compromised ability of those sperm that were produced to undergo capacitation prior to fertilization. An analysis of key signal transduction proteins indicated that FGFR-1 is functional on wild-type sperm and probably signals via the phosphatidylinositol 3-kinase pathway. FGFR-1 activation also resulted in the downstream suppression of mitogen activated protein kinase signaling. These data demonstrate the FGFR-1 is required for quantitatively and qualitatively normal spermatogenesis and has a key role in the regulation of the global tyrosine phosphorylation events associated with sperm capacitation.

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Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 3: Developmental changes in spermatid flagellum and cytoplasmic droplet and interaction of sperm with the zona pellucida and egg plasma membrane

Hermo¹,

Pelletier²,

Cyr³

et al. 2009

Microscopy Res & Technique

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Spermiogenesis constitutes the steps involved in the metamorphosis of spermatids into spermatozoa. It involves modification of several organelles in addition to the formation of several structures including the flagellum and cytoplasmic droplet. The flagellum is composed of a neck region and middle, principal, and end pieces. The axoneme composed of nine outer microtubular doublets circularly arranged to form a cylinder around a central pair of microtubules is present throughout the flagellum. The middle and principal pieces each contain specific components such as the mitochondrial sheath and fibrous sheath, respectively, while outer dense fibers are common to both. A plethora of proteins are constituents of each of these structures, with each playing key roles in functions related to the fertility of spermatozoa. At the end of spermiogenesis, a portion of spermatid cytoplasm remains associated with the released spermatozoa, referred to as the cytoplasmic droplet. The latter has as its main feature Golgi saccules, which appear to modify the plasma membrane of spermatozoa as they move down the epididymal duct and hence may be partly involved in male gamete maturation. The end product of spermatogenesis is highly streamlined and motile spermatozoa having a condensed nucleus equipped with an acrosome. Spermatozoa move through the female reproductive tract and eventually penetrate the zona pellucida and bind to the egg plasma membrane. Many proteins have been implicated in the process of fertilization as well as a plethora of proteins involved in the development of spermatids and sperm, and these are high lighted in this review.

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Tpx-1 is a component of the outer dense fibers and acrosome of rat spermatozoa

Cited by 83 publications

References 37 publications

Cysteine‐rich secretory proteins are not exclusively expressed in the male reproductive tract

Cysteine‐rich secretory proteins are not exclusively expressed in the male reproductive tract

FGFR-1 signaling is involved in spermiogenesis and sperm capacitation

Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 3: Developmental changes in spermatid flagellum and cytoplasmic droplet and interaction of sperm with the zona pellucida and egg plasma membrane

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