Trabecular bone organoid model for studying the regulation of localized bone remodeling

Abstract

Trabecular bone maintains physiological homeostasis and consistent structure and mass through repeated cycles of bone remodeling by means of tightly localized regulation. The molecular and cellular processes that regulate localized bone remodeling are poorly understood because of a lack of relevant experimental models. A tissue-engineered model is described here that reproduces bone tissue complexity and bone remodeling processes with high fidelity and control. An osteoid-inspired biomaterial—demineralized bone paper—directs osteoblasts to deposit structural mineralized bone tissue and subsequently acquire the resting-state bone lining cell phenotype. These cells activate and shift their secretory profile to induce osteoclastogenesis in response to chemical stimulation. Quantitative spatial mapping of cellular activities in resting and activated bone surface coculture showed that the resting-state bone lining cell network actively directs localized bone remodeling by means of paracrine signaling and cell-to-cell contact. This model may facilitate further investigation of trabecular bone niche biology.

Occlusal Trauma and Bisphosphonate-Related Osteonecrosis of the Jaw in Mice

“…The C-MSCs float in medium in an organoid-like 3D structure, while OIM-induced osteogenic differentiation leads to increasing ALP activity, calcium content, and calcium deposition [37]; thus, we presumed that C-MSCs could serve as an in vitro bone-like model. Hydrostatic pressure loading is a simple but well-established system; many reports have described its effects on cultured cells [27] and tissues [38]. LDH is released into the extracellular space when the cell membrane is damaged and is used to detect necrosis in vitro.…”

Therapeutic strategies of three-dimensional stem cell spheroids and organoids for tissue repair and regeneration

“…Bone is a dynamic and multifunctional tissue that exhibits control of mineral homeostasis, blood formation, and mechanical structure in response to changing physical stress and physiological needs [ 164 ]. In the process of bone formation, mechanical and structural stimulation, cell differentiation, and the generation of mineralized organic matrix are synchronized to create hybrid hierarchical structures [ 165 ].…”

Damage Function of a Quasi-Brittle Material, Damage Rate, Acceleration and Jerk during Uniaxial Compression: Model and Application to Analysis of Trabecular Bone Tissue Destruction

“…More derivatives can be used to account for more factors. For this, using Equation (30), we rewrote Equation (31) in the general form (32): Ψ = Ψ(ε)= sum(absD (1) + absD (2) + . .…”

“…The main focus in this study was given to the substantiation of the damage function of a quasi-brittle material, modeling the kinetics of destruction during uniaxial compression on the example of trabecular bone tissue. Trabecular bone is a multifunctional tissue that maintains a dynamic balance of structure and functions through repeated cycles of remodeling and adaptation to changes in physical activity and physiological needs [2][3][4]. The structure and functions of the trabecular bone tissue have been studied for hundreds of years [5], but a number of problems remain relevant [6].…”