Trace level enrichment and clean-up of β-lactam antibiotic residues in edible chicken tissues and feathers by solid phase extraction for quantitative determination utilizing liquid chromatography

Abstract

ABSTRACT. A simple analytical method for the extraction and determination of three β-lactam antibiotics in chicken tissues and feather samples was developed and validated using solid phase extraction (SPE) and liquid chromatography-ultraviolet, LC-UV, detection. The analytes were extracted with strong anion exchange (SAX) cartridges for the extract clean-up and enrichment before introduction into the LC system. The validation was carried out based on the recommendations from Commission Decision 2002/657/EC. Under optimised conditions, the proposed method offers correlation coefficients of r2 ≥ 0.9991 for all the studied analytes in both matrices. Percent recovery results were found to vary between 79.7-101.8% and 94.2-101.6% for chicken feathers and tissues, respectively, with the relative standard deviation, RSD < 8.4%. The limits of detection (LOD) were in the range of 0.1-0.13 µg kg-1 and 0.1-0.15 µg kg-1 while the limits of quantification (LOQ) were in the range of 0.3-0.38 µg kg-1 and 0.3-4.5 µg kg-1 in chicken feathers and tissues, respectively. The proposed method offers good sensitivity and selectivity and all the results demonstrating that the method would be widely used for determination of β-lactam antibiotics and other trace level antibiotics residues having similar physical and chemical properties in the matrices of varying origins.
 
 KEY WORDS: Chicken tissues, Chicken feathers, β-Lactams, Solid phase extraction, LC-UV
 
 Bull. Chem. Soc. Ethiop. 2022, 36(3), 503-519. 
 DOI: https://dx.doi.org/10.4314/bcse.v36i3.2

A selective dispersive liquid-liquid micro-extraction technique for trace level pollutants enrichment of pharmaceutical residues from hospital wastewaters followed by liquid chromatographic analysis

“…Commonly, at low dispersive solvent volume, the tiny droplet of extraction solvent may not be formed effectively, thereby lowering the extraction efficiency. On the other hand, at higher volumes of the dispersive solvent solubility of the analytes in sample solution increased, which may further lower the partitioning of the analytes into the droplets of extraction solvent leading to decreased extraction efficiency [31]. Therefore, different volumes of the dispersive solvent (ranging from 600 to 1900 μL) were tested.…”