Trace metals speciation by HPLC with plasma source mass spectrometry detection.

Abstract

The analysis of environmental and biological samples often requires detection at the parts per billion (ppb) level. Plasma source mass spectrometry has potential as a method for the analysis and speciation of trace elements. This is due to the technique's highly selective nature and excellent sensitivity. In comparison to atomic emission detection, detection limits are usually two to three orders of magnitude lower for plasma MS determinations. Interfacing HPLC with plasma MS provides a means of separation that is necessary for speciation. Speciation involves the determination and quantitation of the various chemical forms of a particular element. A host of HPLC-ICP-MS techniques may be used to obtain this information. This brief report will focus on the most recent work in this area, with emphasis on the work done in our laboratory. -Environ Health Perspect 1 03(Suppl 1): 21-23 (1995)

The Contribution of Inductively Coupled Plasma Mass Spectrometry to Biomedical Research

“…Initial work in this field has been carried out using SEC for the identification and quantification of metalloproteins in various biological samples, such as the speciation of Au, Cu, Zn, and Pb in blood, Cd in kidney, and Pb, Cu, Zn, and Fe in blood serum (6,35). Speciation of toxicologically important elements such as As, Cr, Pb, Hg, Ni, and V in urine using ion-exchange and ion-pairing chromatography have also been reported (6,35,36,38).…”