Traceability validation of six enzyme measurements on the Abbott Alinity c analytical system

Aloisio, Elena; Frusciante, Erika; Pasqualetti, Sara; Infusino, Ilenia; Krintus, Magdalena; Sypniewska, Grażyna; Panteghini, Mauro

doi:10.1515/cclm-2020-0015

Cited by 14 publications

(5 citation statements)

References 34 publications

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“…According to the PRORATA trial [17], in ICU patients with lower respiratory tract infections, PCT values <0.25 μg/L indicate bacterial etiology as very unlikely, while values ≥1.0 μg/L indicate a high probability for this etiology. CRP, serum albumin, and LDH were determined on the Alinity c platform (Abbott Diagnostics) by using immunoturbidimetry (CRP and albumin) and enzymatic (LDH) assays [18][19][20]. The mean CVs in the study period were 3.1% for CRP, 1.8% for albumin, and 1.2% for LDH, at a mean analyte concentration of 12.0 mg/L (CRP), 39.6 g/L (albumin), and 395 U/L (LDH), respectively.…”

Section: Methodsmentioning

confidence: 99%

Searching for a role of procalcitonin determination in COVID-19: a study on a selected cohort of hospitalized patients

Dolci

Robbiano

Aloisio

et al. 2020

Clinical Chemistry and Laboratory Medicine (CCLM)

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ObjectivesProcalcitonin (PCT) has been proposed for differentiating viral vs. bacterial infections. In COVID-19, some preliminary results have shown that PCT testing could act as a predictor of bacterial co-infection and be a useful marker for assessment of disease severity.MethodsWe studied 83 COVID-19 hospitalized patients in whom PCT was specifically ordered by attending physicians. PCT results were evaluated according to the ability to accurately predict bacterial co-infections and death in comparison with other known biomarkers of infection and with major laboratory predictors of COVID-19 severity.ResultsThirty-three (39.8%) patients suffered an in-hospital bacterial co-infection and 44 (53.0%) patients died. In predicting bacterial co-infection, PCT showed a relatively low accuracy (area under receiver-operating characteristic [ROC] curve [AUC]: 0.757; 95% confidence interval [CI]: 0.651–0.845), with a strength for detecting the outcome not significantly different from that of white blood cell count and C-reactive protein (CRP). In predicting patient death, PCT showed an AUC of 0.815 (CI: 0.714–0.892), not better than those of other more common laboratory tests, such as blood lymphocyte percentage (AUC: 0.874, p=0.19), serum lactate dehydrogenase (AUC: 0.860, p=0.47), blood neutrophil count (AUC: 0.845, p=0.59), and serum albumin (AUC: 0.839, p=0.73).ConclusionsProcalcitonin (PCT) testing, even when appropriately ordered, did not provide a significant added value in COVID-19 patients when compared with more consolidated biomarkers of infection and poor clinical outcome. The major application of PCT in COVID-19 is its ability, associated with a negative predictive value >90%, to exclude a bacterial co-infection when a rule-out cut-off (<0.25 μg/L) is applied.

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Section: Methodsmentioning

confidence: 99%

Searching for a role of procalcitonin determination in COVID-19: a study on a selected cohort of hospitalized patients

Dolci

Robbiano

Aloisio

et al. 2020

Clinical Chemistry and Laboratory Medicine (CCLM)

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“…The liver is a metabolic organ in the body and plays a role to oxidize, store glycogen and secrete proteins [ 30 ]. The liver also produces bile in the digestive system [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

Protective Mechanism of Polygonum perfoliatum L. Extract on Chronic Alcoholic Liver Injury Based on UHPLC-QExactive Plus Mass Spectrometry Lipidomics and MALDI-TOF/TOF Mass Spectrometry Imaging

Chen

Peng

Zhao

et al. 2022

Foods

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Polygonum perfoliatum L. has a long history of medicinal and edible applications. Studies have shown that it can significantly protect liver injury, but the mechanism is unclear. The purpose of this study was to explore the protective mechanism of P. perfoliatum on chronic alcoholic liver injury from the perspective of lipid metabolism. After 8 weeks of alcohol exposure in male Wister mice, the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) in serum were significantly increased, and the activities of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) in liver were significantly decreased. Meanwhile, pathological changes of liver tissue in mice were observed by histopathology. Then, Ultra-High Performance Liquid Chromatography (UHPLC) QExactive Plus Mass Spectrometer lipidomics and matrix-assisted laser desorption/ionization time-of-flight/time -of-flight (MALDI-TOF/TOF) mass spectrometry imaging methods were established to analyze lipid metabolism in mice. Ten different lipids were identified by statistical analysis, including Fatty Acyls, Glycerophospholipids, Prenol lipids and Sphingomyelins. After intervention with P. perfoliatum extracts at different doses (25 to 100 mg/kg), levels of AST, ALT, ALP in serum, and activities of ADH and ALDH in liver were significantly corrected. The hepatic cord structure was clear, and the liver cells were closely arranged without other obvious abnormalities. Non-target lipidomics analysis showed that P. perfoliatum extract could regulate the metabolic disorders of the 10 different lipids caused by continuous alcohol exposure. Pathway analysis suggested that the mechanism of P. perfoliatum extract on chronic alcoholic liver injury may be related to the regulation of linoleic acid and α-linolenic acid.

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“…Aloisio et al 23 suggested that laboratory professionals should independently verify the correct implementation of metrological traceability of measuring systems. The EQA/PT scheme not only plays an important role in evaluating the performance of each laboratory but also has unique efficacy in monitoring the equivalence of measurement results and the success of standardization 24 .…”

Section: Discussionmentioning

confidence: 99%

EQA/PT scheme to improve the equivalence of enzymatic results between mutual recognition laboratories in Beijing

Tong

Zhang

Zuo

2021

Clinical Laboratory Analysis

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Background To utilize the external quality assessment (EQA)/proficiency testing (PT) scheme to evaluate the equivalence of different clinical enzymatic measuring systems in Beijing. Methods The Beijing Center for Clinical Laboratory (BCCL) distributed three investigation samples to mutual recognition clinical laboratories in Beijing including alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ‐glutamyltransferase (GGT), creatine kinase (CK), and lactate dehydrogenase (LDH). These samples were derived from serum pools with values assigned by the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) enzymatic reference measurement procedures (RMPs). Each laboratory performed duplicate tests of the samples. Then, the samples at level 1 were used to recalibrate individual measuring systems for repeating the tests. BCCL collected data for evaluation of their analytical quality. Results Before recalibration, the biases of ALT and AST tests were not traceable to the IFCC RMPs, and the bias pass rates of GGT, CK, and LDH tests were only 51.2%, 55.7%, and 48.6% respectively. After recalibration, the pass rates of ALT, AST, GGT, CK, and LDH increased to 95.1%, 82.9%, 95.1%, 97.1%, and 70.0% respectively. The EQA/PT also showed that after recalibration, more than 95% of laboratories met the optimum level specifications of the biological variation for ALT, AST, GGT, and CK tests and the desirable for LDH tests. Conclusion The enzymatic tests in Beijing need to be further standardized by category 1 or 2 EQA/PT scheme for mutual recognition between clinical laboratories. The criteria of biological variation are more relevant for determining the equivalence of clinical enzymatic tests.

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Traceability validation of six enzyme measurements on the Abbott Alinity c analytical system

Cited by 14 publications

References 34 publications

Searching for a role of procalcitonin determination in COVID-19: a study on a selected cohort of hospitalized patients

Searching for a role of procalcitonin determination in COVID-19: a study on a selected cohort of hospitalized patients

Protective Mechanism of Polygonum perfoliatum L. Extract on Chronic Alcoholic Liver Injury Based on UHPLC-QExactive Plus Mass Spectrometry Lipidomics and MALDI-TOF/TOF Mass Spectrometry Imaging

EQA/PT scheme to improve the equivalence of enzymatic results between mutual recognition laboratories in Beijing

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