Tracheal Resection and Reconstruction for Malignant Tumor

Parshad, Sanjeev; Gogna, Shekhar; Saroha, Vikas; Lohchab, Shamsher Singh; Karwasra, R K

doi:10.1007/s13193-020-01124-4

Cited by 1 publication

(1 citation statement)

References 7 publications

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“…In case TRA is not feasible, identification of effective transplantation options, free from need of immunosuppressant therapies to preserve grafted tissue structural/functional integrity, are an ambitious goal to pursue. Together with eliminating immunosuppressant therapy side effects and the related costs, they would guarantee for significant improvements of patients’ life quality, especially (but not limited) to the oncologic category [ 11 , 56 , 57 , 58 ].…”

Section: Discussionmentioning

confidence: 99%

Development and In Vitro/In Vivo Comparative Characterization of Cryopreserved and Decellularized Tracheal Grafts

Stocco

Barbon

Mammana

et al. 2023

Cells

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Tracheal reconstruction represents a challenge when primary anastomosis is not feasible. Within this scenario, the study aim was to develop a new pig-derived decellularized trachea (DecellT) to be compared with the cryopreserved counterpart (CryoT) for a close predictive analysis. Tracheal segments underwent decellularization by a physical + enzymatic + chemical method (12 cycles); in parallel, cryopreserved samples were also prepared. Once decellularized (histology/DNA quantification), the two groups were characterized for Alpha-Gal epitopes/structural proteins (immunohistochemistry/histology/biochemical assays/second harmonic generation microscopy)/ultrastructure (Scanning Electron Microscopy (SEM))/mechanical behaviour. Cytotoxicity absence was assessed in vitro (extract-test assay/direct seeding, HM1SV40 cell line) while biocompatibility was verified in BALB/c mice, followed by histological/immunohistochemical analyses and SEM (14 days). Decellularization effectively removed Alpha-Gal epitopes; cartilage histoarchitecture was retained in both groups, showing chondrocytes only in the CryoT. Cryopreservation maintained few respiratory epithelium sparse cilia, not detectable in DecellT. Focusing on ECM, preserved structural/ultrastructural organization and collagen content were observed in the cartilage of both; conversely, the GAGs were significantly reduced in DecellT, as confirmed by mechanical study results. No cytotoxicity was highlighted by CryoT/DecellT in vitro, as they were also corroborated by a biocompatibility assay. Despite some limitations (cells presence/GAGs reduction), CryoT/DecellT are both appealing options, which warrant further investigation in comparative in vivo studies.

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