Tracing lineage by phenotypic and genotypic markers in Salmonella enterica subsp. enterica serovar 1,4,[5],12:i:- and Salmonella Typhimurium isolated in state of São Paulo, Brazil

Tavechio, Ana Terezinha; Fernandes, Sueli Aparecida; Ghilardi, Angela C. R.; Soule, Geoff; Ahmed, Rafiq; Melles, Carmo Elias Andrade

doi:10.1590/s0074-02762009000700019

Cited by 15 publications

(14 citation statements)

References 21 publications

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“…Typhimurium. The literature confirmed that DT2 and DT99 have never been associated with monophasic isolates (Echeita et al., 2001; Tavechio et al., 2009; EFSA, 2010. ; Hauser et al., 2010), whereas a S .…”

Section: Discussionmentioning

confidence: 88%

Application of the Random Forest Method to Analyse Epidemiological and Phenotypic Characteristics of Salmonella 4,[5],12:i:‐ and Salmonella Typhimurium Strains

Barco

Mancin

Ruffa

et al. 2012

Zoonoses and Public Health

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Salmonella enterica 4,[5],12:i:- is a monophasic variant of S. Typhimurium. In the last decade, its prevalence rose sharply. Although S. 4,[5],12:i:- and S. Typhimurium are known to pose a considerable public health risk, there is no detailed information on the circulation of these serovars in Italy, particularly as far as veterinary isolates are concerned. For this reason, a data set of 877 strains isolated in the north-east of Italy from foodstuffs, animals and environment was analysed during 2005-2010. The Random Forests (RF) method was used to identify the most important epidemiological and phenotypic variables to show the difference between the two serovars. Both descriptive analysis and RF revealed that S. 4,[5],12:i:- is less heterogeneous than S. Typhimurium. RF highlighted that phage type was the most important variable to differentiate the two serovars. The most common phage types identified for S. 4,[5],12:i:- were DT20a, U311 and DT193. The same phage types were also found in S. Typhimurium isolates, although with a much lower prevalence. DT7 and DT120 were ascribed to the two serovars at comparable levels. DT104, DT2 and DT99 were ascribed exclusively to S. Typhimurium, and almost all the other phage types identified were more related to the latter serovar. Such data confirm that phage typing can provide an indication of the biphasic or monophasic state of the strains investigated and could therefore support serotyping results. However, phage typing cannot be used as the definitive method to differentiate the two serovars, as part of the phage types were detected for both serovars and, in particular, all phage types found for S. 4,[5],12:i- were found also for S. Typhimurium.

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Section: Discussionmentioning

confidence: 88%

Application of the Random Forest Method to Analyse Epidemiological and Phenotypic Characteristics of Salmonella 4,[5],12:i:‐ and Salmonella Typhimurium Strains

Barco

Mancin

Ruffa

et al. 2012

Zoonoses and Public Health

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“…Además de Estados Unidos, en países del resto de América como Canadá y Brasil, la STVM también se encuentra en los cinco primeros lugares de aislamientos provenientes de muestras de humanos, en tanto que el 100 % de los aislamientos de animales de engorde en estos países corresponde a dicha variante (33,34), En Argentina solo existe un reporte reciente de su presencia en granjas de cerdos (35). En Colombia, en solo cinco años desde su primera identificación en aislamientos clínicos, la variante monofásica se ubicó en el cuarto lugar de la vigilancia, lo que evidencia su capacidad de diseminación (14).…”

Section: Discussionunclassified

Caracterización molecular y fenotípica de aislamientos clínicos de Salmonella Typhimurium variante monofásica (1,4,[5],12:i:-) recuperados en Colombia

et al. 2020

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Introducción. La variante monofásica (1,4,[5],12:i:-) de Salmonella Typhimurium ocupa los primeros lugares en los programas de vigilancia de Salmonella a nivel mundial. En Colombia, Salmonella enterica variante monofásica alcanza el cuarto lugar en cuanto a los aislamientos clínicos recuperados por medio de la vigilancia por laboratorio del Grupo de Microbiología del Instituto Nacional de Salud, pero se desconoce si dichos aislamientos están relacionados con la variante monofásica de Typhimurium que circula a nivel global, y con sus características genéticas y fenotípicas.Objetivo. Caracterizar los aislamientos de Salmonella monofásica recuperados en Colombia entre el 2015 y el 2018 por el Grupo de Microbiología del Instituto Nacional de Salud.Materiales y métodos. Se analizaron 286 aislamientos clínicos de Salmonella enterica variante monofásica mediante PCR o secuenciación del genoma completo (Whole Genome Sequencing, WGS) para confirmar si correspondían a Salmonella Typhimurium variante monofásica, en tanto que, en 54 aislamientos, se determinó la estructura genética del operón que codifica la segunda fase flagelar y, en 23, se evaluó la motilidad, el crecimiento y la expresión de las proteínas de membrana externa.Resultados. El 61 % (n=174) de los aislamientos de Salmonella monofásica correspondió a Salmonella Typhimurium serovar monofásico. El 64,8 % (n=35/54) se relacionó con el clon europeo-español y, el 13 % (n=7/54), con el estadounidense. En dos aislamientos de orina se encontró una diferencia significativa en la motilidad y el crecimiento, así como ausencia de la porina OmpD en medio mínimo M9.Conclusiones. En el periodo de estudio, circuló en Colombia la variante monofásica de Salmonella Typhimurium relacionada con el clon europeo-español, y se registró ausencia total del operón fljAB. Los resultados evidenciaron cambios fenotípicos en los aislamientos provenientes de muestras de orina que sugieren adaptación en procesos invasivos.

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“…S. I. 4, [5],12:i:-has been responsible for an increasing number of foodborne outbreaks, attributed to pigs and pork products and frequently associated with the emergence of the multidrug-resistant phenotype 21,23 .…”

Section: Distribution Of Salmonella Serotypes From Human Infectionsmentioning

confidence: 99%

Salmonella enterica serotypes from human and nonhuman sources in Sao Paulo State, Brazil, 2004-2020

Fernandes

Tavechio

Ghilardi

et al. 2022

Rev. Inst. Med. trop. S. Paulo

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Salmonellosis ranks among the most frequently reported zoonosis worldwide and is often associated with foodborne outbreaks. Since the 1950s, the distribution of Salmonella serotypes in Sao Paulo State, Brazil, has been documented and periodically reported. In this study, we updated the data on the distribution of Salmonella serotypes received in our reference laboratory, isolated from human infections and nonhuman sources, from 2004 to 2020. In that period, a total of 9,014 Salmonella isolates were analyzed, of which 3,553 (39.4%) were recovered from human samples, mainly of stool (65%) and blood (25.6%), and 5,461 (60.6%) were isolated from nonhuman origins, such as animals (47.2%), food (27.7%) and animal environments (18.6%). In human isolates, a total of 104 serotypes were identified and the most frequent ones were Enteritidis, Typhimurium, S. I. 4,[5],12:i:-, Dublin and Typhi. A consistent reduction of the Enteritidis proportion was observed over the years. Among the 156 serotypes identified in isolates with nonhuman origins, Enteritidis, Mbandaka, Typhimurium, Agona and Anatum were ranked as the top five Salmonella serotypes; in more recent years, S. Heidelberg has increased in frequency. Although with different proportions, the top 10 prevalent serotypes were identified in both human and nonhuman origins, underscoring the role of animals, food products and environment as reservoirs of Salmonella with potential to cause human salmonellosis.

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Tracing lineage by phenotypic and genotypic markers in Salmonella enterica subsp. enterica serovar 1,4,[5],12:i:- and Salmonella Typhimurium isolated in state of São Paulo, Brazil

Abstract: Fifty-three Salmonella 1,4,[5],

Cited by 15 publications

References 21 publications

Application of the Random Forest Method to Analyse Epidemiological and Phenotypic Characteristics of Salmonella 4,[5],12:i:‐ and Salmonella Typhimurium Strains

Application of the Random Forest Method to Analyse Epidemiological and Phenotypic Characteristics of Salmonella 4,[5],12:i:‐ and Salmonella Typhimurium Strains

Caracterización molecular y fenotípica de aislamientos clínicos de Salmonella Typhimurium variante monofásica (1,4,[5],12:i:-) recuperados en Colombia

Salmonella enterica serotypes from human and nonhuman sources in Sao Paulo State, Brazil, 2004-2020

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